Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging-drop vapour-diffusion method, 0.001 ml of 15 mg/ml protein solution is mixed with 0.001 ml of reservoir solution containing 8% PEG 8000 and 0.1 M TrisHCl pH 8.0., at 4°C or 20°C, crystals appear within 24 h, X-ray diffraction structure determination and analysis at 2.3 A resolution | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Staphylococcus aureus | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration | Staphylococcus aureus |
Storage Stability | Organism |
---|---|
-80°C, 1-2 mg/ml purified recombinant His6-tagged enzyme, in 50 mM Tris-HCl, pH 7.5, 0.5 mM EDTA, pH 8.0, 250 mM NaCl, at least 6 months without loss of activity | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Staphylococcus aureus | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
additional information | biotin protein ligase from Staphylococcus aureus catalyses the biotinylation of acetyl-CoA carboxylase and pyruvate carboxylase | Staphylococcus aureus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
biotin protein ligase | - |
Staphylococcus aureus |
BPL | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Staphylococcus aureus |