6.3.4.15: biotin-[biotin carboxyl-carrier protein] ligase
This is an abbreviated version!
For detailed information about biotin-[biotin carboxyl-carrier protein] ligase, go to the full flat file.
Word Map on EC 6.3.4.15
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6.3.4.15
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biotinylation
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streptavidin
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bioid
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proximity-dependent
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bap
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avidin
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avitag
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streptavidin-coated
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biotin-dependent
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transcarboxylase
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proximity-labeling
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biotinyl-5\'-amp
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drug development
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unbiotinylated
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15-amino
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biotin-streptavidin
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diagnostics
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analysis
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synthesis
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carboxylases
- 6.3.4.15
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biotinylation
- streptavidin
-
bioid
-
proximity-dependent
- bap
- avidin
-
avitag
-
streptavidin-coated
-
biotin-dependent
- transcarboxylase
-
proximity-labeling
-
biotinyl-5\'-amp
- drug development
-
unbiotinylated
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15-amino
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biotin-streptavidin
- diagnostics
- analysis
- synthesis
- carboxylases
Reaction
Synonyms
Acetyl CoA holocarboxylase synthetase, Acetyl coenzyme A holocarboxylase synthetase, bacterial BirA biotin ligase, biotin acetyl-CoA carboxylase ligase, Biotin holoenzyme synthetase, biotin ligase, biotin protein ligase, Biotin--protein ligase, biotin-protein ligase, Biotin-[acetyl coenzyme A carboxylase] synthetase, Biotin-[acetyl-CoA carboxylase] synthetase, Biotin:apocarboxylase ligase, BirA, BirA protein, BPL, group I biotin protein ligase, HCS, Holocarboxylase synthetase, holocarboxylase synthetase 1, More, STK_15250, Synthetase, biotin-[acetyl coenzyme A carboxylase], yBL
ECTree
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KM Value
KM Value on EC 6.3.4.15 - biotin-[biotin carboxyl-carrier protein] ligase
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0.0052
apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
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pH 8.0, 37°C
0.12
[Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine
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pH 8.0, 37°C, wild type enzyme
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0.011
[Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine
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pH 8.0, 37°C, wild type enzyme
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0.03 - 0.101
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
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0.26
biotin
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reaction with ATP + apo-carboxyl carrier protein, a subunit of acetyl-CoA carboxylase from E. coli
0.03
pH 8.0, 37°C, wild type enzyme
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0.063
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
pH 8.0, 70°C, mutant enzyme A43G
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0.101
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
pH 8.0, 70°C, wild type enzyme
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0.00044
pH 8.0, 70°C, wild type enzyme
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0.00058
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine
pH 8.0, 70°C, mutant enzyme A43G
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0.00058
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine
pH 8.0, 70°C, wild type enzyme
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additional information
additional information
isothermal titration calorimetry measurements of wild-type an dR40G mutant BPL with biotin and ATP, ATP and biotin binding are enthalpydriven with a favourable entropy change, overview
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additional information
additional information
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isothermal titration calorimetry measurements of wild-type an dR40G mutant BPL with biotin and ATP, ATP and biotin binding are enthalpydriven with a favourable entropy change, overview
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