6.2.1.20: long-chain-fatty-acid-[acyl-carrier-protein] ligase

This is an abbreviated version!
For detailed information about long-chain-fatty-acid-[acyl-carrier-protein] ligase, go to the full flat file.

Word Map on EC 6.2.1.20

Reaction

ATP
+
a long-chain fatty acid
+
an [acyl-carrier protein]
=
AMP
+
diphosphate
+
a long-chain acyl-[acyl-carrier protein]

Synonyms

Synthetase, acyl-[acyl carrier protein], Acyl-[acyl carrier protein] synthetase, Acyl-ACP synthetase, Acyl-[acyl-carrier-protein]synthetase, Stearoyl-ACP synthetase, Acyl-acyl carrier protein synthetase, Acyl-CoA synthetase, Acyl-CoA ligase, AcpS, LipA, acyl carrier protein synthase, PfACP, At4g14070, ACSL, AasS, AAS, AAE15, acyl-ACP synthase, CT776, slr1609, AasC, ACP synthase, CTO_0846

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.20 long-chain-fatty-acid-[acyl-carrier-protein] ligase

Specific Activity

Specific Activity on EC 6.2.1.20 - long-chain-fatty-acid-[acyl-carrier-protein] ligase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000478
-
at 37°C for 30 min with 10 mM MgSO4, 10 mM ATP, 0.1 mM [9,10-3H]myristic acid in 100 mM Tris-HCl, pH 7.8, and 5 mM dithiothreitol
0.0000667
purified recombinant enzyme, pH 8.0, 37°C, with substrate decanoate
0.0001027
purified recombinant enzyme, pH 8.0, 37°C, with substrate laurate
0.0001572
purified recombinant enzyme, pH 8.0, 37°C, with substrate myristate
0.022
-
transfected Escherichia coli BL21(DE3) with lactose as inducer and palmitic acid as substrate
0.028
-
-
0.071
-
transfected Escherichia coli C41(DE3) with IPTG as inducer and palmitic acid as substrate
1.32
-
purified enzyme from Escherichia coli C41(DE3) with palmitic acid as substrate