6.2.1.20: long-chain-fatty-acid-[acyl-carrier-protein] ligase

This is an abbreviated version!
For detailed information about long-chain-fatty-acid-[acyl-carrier-protein] ligase, go to the full flat file.

Word Map on EC 6.2.1.20

6.2.1.20

harveyi

4'-phosphopantetheine

holo-acp

4'-phosphopantetheinylated

pptases

lignoceric

lignoceroyl-coa

synthesis

biofuel production

Reaction

a long-chain acyl-[acyl-carrier protein]

Synonyms

AAE15, AAS, AasC, AasS, ACP synthase, AcpS, ACSL, acyl carrier protein synthase, acyl-ACP synthase, Acyl-ACP synthetase, Acyl-acyl carrier protein synthetase, Acyl-CoA ligase, Acyl-CoA synthetase, Acyl-[acyl carrier protein] synthetase, Acyl-[acyl-carrier-protein]synthetase, At4g14070, CT776, CTO_0846, LipA, PfACP, slr1609, Stearoyl-ACP synthetase, Synthetase, acyl-[acyl carrier protein]

ECTree

6 Ligases

6.2 Forming carbon-sulfur bonds

6.2.1 Acid-thiol ligases

6.2.1.20 long-chain-fatty-acid-[acyl-carrier-protein] ligase

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Results	in table
3	Activating Compound
2369	AA Sequence
5	Application
2	CAS Registry Number
10	Cloned(Commentary)
7	Cofactor
22	General Information
4	General Stability
6	Inhibitors
24	KM Value [mM]
15	Localization
16	Metals/Ions
6	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
52	Organism
6	Pathway
6	PDB ID
4	pH Optimum
6	Protein Variants
12	Purification (Commentary)
1	Reaction
3	Reaction Type
33	Reference
3	Source Tissue
9	Specific Activity [micromol/min/mg]
3	Storage Stability
97	Substrates and Products (Substrate)
4	Subunits
68	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Stability [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 6.2.1.20 - long-chain-fatty-acid-[acyl-carrier-protein] ligase

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2 entries

a single enzyme protein catalyzes two activities: 2-acylglycerophosphoethanolamine acyltransferase activity and acyl-[acyl-carrier protein] synthetase activity

Escherichia coli

685

Ni-NTA column chromatography

partial

690, 645410, 654429

partial purification by DEAE-Sepharose chromatography and Sephacryl S-300 gel filtration

Vibrio harveyi

674883

recombinant His-tagged enzyme from Escherichia coli

Vibrio harveyi

Q00IB3

744979

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) Rosetta to over 90% purity, purified AasC catalyzed the ATP-dependent ligation of free fatty acids to ACP

Chlamydia trachomatis

O84781

745328

recombinant N-terminally His6-tagged enzyme, full-length or lacking the predicted plastidial targeting signal sequence, from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography, gel filtration, and dialysis. Removing the sequence encoding a predicted plastidial targeting peptide increases the expression level significantly, like DELTAaas, DELTAaas:AAE15 secretes large amounts of fatty acids into the culture medium. Expression of AAE15 in the DELTAaas strain does not affect fatty acid profiles and does not have a significant complementation effect

Arabidopsis thaliana

Q8W471

743996

recombinant proteins using His-tag

Escherichia coli

657235