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6.2.1.20: long-chain-fatty-acid-[acyl-carrier-protein] ligase

This is an abbreviated version!
For detailed information about long-chain-fatty-acid-[acyl-carrier-protein] ligase, go to the full flat file.

Word Map on EC 6.2.1.20

Reaction

ATP
+
a long-chain fatty acid
+
an [acyl-carrier protein]
=
AMP
+
diphosphate
+
a long-chain acyl-[acyl-carrier protein]

Synonyms

AAE15, AAS, AasC, AasS, ACP synthase, AcpS, ACSL, acyl carrier protein synthase, acyl-ACP synthase, Acyl-ACP synthetase, Acyl-acyl carrier protein synthetase, Acyl-CoA ligase, Acyl-CoA synthetase, Acyl-[acyl carrier protein] synthetase, Acyl-[acyl-carrier-protein]synthetase, At4g14070, CT776, CTO_0846, LipA, PfACP, slr1609, Stearoyl-ACP synthetase, Synthetase, acyl-[acyl carrier protein]

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.20 long-chain-fatty-acid-[acyl-carrier-protein] ligase

Cloned

Cloned on EC 6.2.1.20 - long-chain-fatty-acid-[acyl-carrier-protein] ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) and C41(DE3)
-
expressed in Escherichia coli strain BL21(DELTADE3)
gene AAE15 or At4g14070, recombinant expression of N-terminally His6-tagged enzyme, full-length or lacking the predicted plastidial targeting signal sequence, in Spodoptera frugiperda Sf9 cells via baculovirus transfection method, recombinant expression of Arabidopsis thaliana AAE15 in an AAS deletion mutant of Synechocystis sp. PCC 6803 resulting in changes in intracellular and extracellular free fatty acids pools in the cyanobacterial DELTAaas strain
gene aasC, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Rosetta, complementation of Escherichia coli strain LCH66 (aas-1 pldA1 pldB12)
gene aasS, recombinant expression of His-tagged enzyme in Escherichia coli
gene slr1609, recombinant expression of enzyme AasC in Escherichia coli
overexpression in Escherichia coli
-