Cloned (Comment) | Organism |
---|---|
gene AAE15 or At4g14070, recombinant expression of N-terminally His6-tagged enzyme, full-length or lacking the predicted plastidial targeting signal sequence, in Spodoptera frugiperda Sf9 cells via baculovirus transfection method, recombinant expression of Arabidopsis thaliana AAE15 in an AAS deletion mutant of Synechocystis sp. PCC 6803 resulting in changes in intracellular and extracellular free fatty acids pools in the cyanobacterial DELTAaas strain | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a N-terminally truncated enzyme mutant with deleted predicted plastidial targeting signal sequence by removing the sequence encoding a predicted plastidial targeting peptide increases the expression level significantly | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | the enzyme has a predicted plastidial targeting peptide | Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a long-chain fatty acid + an [acyl-carrier protein] | Arabidopsis thaliana | - |
AMP + diphosphate + a long-chain acyl-[acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8W471 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme, full-length or lacking the predicted plastidial targeting signal sequence, from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography, gel filtration, and dialysis. Removing the sequence encoding a predicted plastidial targeting peptide increases the expression level significantly, like DELTAaas, DELTAaas:AAE15 secretes large amounts of fatty acids into the culture medium. Expression of AAE15 in the DELTAaas strain does not affect fatty acid profiles and does not have a significant complementation effect | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000667 | - |
purified recombinant enzyme, pH 8.0, 37°C, with substrate decanoate | Arabidopsis thaliana |
0.0001027 | - |
purified recombinant enzyme, pH 8.0, 37°C, with substrate laurate | Arabidopsis thaliana |
0.0001572 | - |
purified recombinant enzyme, pH 8.0, 37°C, with substrate myristate | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a long-chain fatty acid + an [acyl-carrier protein] | - |
Arabidopsis thaliana | AMP + diphosphate + a long-chain acyl-[acyl-carrier protein] | - |
? | |
ATP + decanoate + an [acyl-carrier protein] | - |
Arabidopsis thaliana | AMP + diphosphate + long-chain decanoyl-[acyl-carrier protein] | - |
? | |
ATP + hexadecanoate + an [acyl-carrier protein] | low activity | Arabidopsis thaliana | AMP + diphosphate + hexadecanoyl-[acyl-carrier protein] | - |
? | |
ATP + laurate + an [acyl-carrier protein] | - |
Arabidopsis thaliana | AMP + diphosphate + lauroyl-[acyl-carrier protein] | - |
? | |
ATP + myristate + an [acyl-carrier protein] | - |
Arabidopsis thaliana | AMP + diphosphate + myristoyl-[acyl-carrier protein] | - |
? | |
ATP + octanoate + an [acyl-carrier protein] | low activity | Arabidopsis thaliana | AMP + diphosphate + octanoyl-[acyl-carrier protein] | - |
? | |
additional information | recombinant enzyme AAE15 has acyl-ACP synthetase activity in vitro with specificity for medium chain fatty acids, substrate specificity, overview. Low activity with fatty acids 18:0, 18:1, 18:2, and 18:3 | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79000, recombinant truncated enzyme lacking the predicted plastidial targeting signal sequence, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AAE15 | - |
Arabidopsis thaliana |
AAS | - |
Arabidopsis thaliana |
Acyl-ACP synthetase | - |
Arabidopsis thaliana |
Acyl-acyl carrier protein synthetase | - |
Arabidopsis thaliana |
At4g14070 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |