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Literature summary for 6.2.1.20 extracted from

  • Fice, D.; Shen, Z.; Byers, D.M.
    Purification and characterization of fatty acyl-acyl carrier protein synthetase from Vibrio harveyi (1993), J. Bacteriol., 175, 1865-1870.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
iodoacetamide stimulates Vibrio harveyi

Inhibitors

Inhibitors Comment Organism Structure
NEM acyl-carrier protein or ATP protects Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
palmitoleic acid E. coli [acyl-carrier protein] Vibrio harveyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
gel filtration under reducing conditions Vibrio harveyi
62000
-
x * 62000, SDS-PAGE Vibrio harveyi

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6
-
-
Vibrio harveyi

Storage Stability

Storage Stability Organism
-20°C, 0.002% Triton X-100, 10% glycerol, 50% loss of activity within 1 month Vibrio harveyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dATP + a fatty acid + [acyl-carrier protein] 25% of the activity relative to ATP Vibrio harveyi dAMP + diphosphate + fatty acid-[acyl-carrier protein]
-
?
ATP + myristic acid + [acyl-carrier protein]
-
Vibrio harveyi AMP + diphosphate + myristoyl-[acyl-carrier protein]
-
?
ATP + palmitoleic acid + [acyl-carrier protein]
-
Vibrio harveyi AMP + diphosphate + palmitoleoyl-[acyl-carrier protein]
-
?

Subunits

Subunits Comment Organism
? x * 62000, SDS-PAGE Vibrio harveyi