6.1.1.21: histidine-tRNA ligase

This is an abbreviated version!
For detailed information about histidine-tRNA ligase, go to the full flat file.

Reaction

Synonyms

antihistidyl-tRNA synthetase, class II histidyl-tRNA synthetase, HARS, HARS1, HARS2, HisRS, HisRS-1, HisRS-2, Histidine translase, Histidine--tRNA ligase, Histidine--tRNA ligase homolog, histidine-tRNA ligase, histidine-tRNA ligase homolog, histidyl tRNA synthetase, Histidyl-transfer ribonucleate synthetase, histidyl-transfer RNA synthetase, Histidyl-tRNA synthetase, HRS, HTS1, Jo-1, Jo-1 antigen, mitochondrial histidyl tRNA synthetase, Synthetase, histidyl-transfer ribonucleate

ECTree

     6 Ligases

         6.1 Forming carbon-oxygen bonds

             6.1.1 Ligases forming aminoacyl-tRNA and related compounds

                6.1.1.21 histidine-tRNA ligase

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Results	in table
2	Activating Compound
49530	AA Sequence
4	Application
1	CAS Registry Number
22	Cloned(Commentary)
36	Cofactor
13	Crystallization (Commentary)
260	Disease/ Diagnostics
1	Expression
14	General Information
12	General Stability
7	IC50 Value
39	Inhibitors
14	kcat/KM [mM/s]
78	KM Value [mM]
22	Localization
19	Metals/Ions
41	Molecular Weight [Da]
63	Natural Substrates/ Products (Substrates)
111	Organism
1	Oxidation Stability
4	Pathway
86	PDB ID
12	pH Optimum
2	pH Range
1	Posttranslational Modification
57	Protein Variants
26	Purification (Commentary)
12	Reaction
62	Reaction Type
101	Reference
1	Renatured (Commentary)
29	Source Tissue
9	Specific Activity [micromol/min/mg]
5	Storage Stability
134	Substrates and Products (Substrate)
36	Subunits
346	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
61	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 6.1.1.21 - histidine-tRNA ligase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, 10 mM Hepes, pH 7.4, 1 mM magnesium acetate, 1 mM KCl, 0.5 mM DTT, 0.1 mM phenylmethylsulfonyl fluoride, 50% glycerol, stable for 6 months

Neurospora crassa

-

374

-20°C, 50% glycerol, 2 mM 2-mercaptoethanol, stable for months

Salmonella enterica subsp. enterica serovar Typhimurium

-

367

-20°C, 50% glycerol, 5 mM 2-mercaproethanol, stable for more than 4 months

Escherichia coli

-

369

-20°C, no loss of activity after 6 months

Salmonella enterica subsp. enterica serovar Typhimurium

-

368

-85°C, stable for many months

Oryctolagus cuniculus

-

373