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6.1.1.21: histidine-tRNA ligase

This is an abbreviated version!
For detailed information about histidine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.21

Reaction

ATP
+
L-histidine
 +
tRNAHis
=
AMP
 +
diphosphate
 +
L-histidyl-tRNAHis

Synonyms

antihistidyl-tRNA synthetase, class II histidyl-tRNA synthetase, HARS, HARS1, HARS2, HisRS, HisRS-1, HisRS-2, Histidine translase, Histidine--tRNA ligase, Histidine--tRNA ligase homolog, histidine-tRNA ligase, histidine-tRNA ligase homolog, histidyl tRNA synthetase, Histidyl-transfer ribonucleate synthetase, histidyl-transfer RNA synthetase, Histidyl-tRNA synthetase, HRS, HTS1, Jo-1, Jo-1 antigen, mitochondrial histidyl tRNA synthetase, Synthetase, histidyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.21 histidine-tRNA ligase

General Stability

General Stability on EC 6.1.1.21 - histidine-tRNA ligase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol stabilizes the enzyme
4°C, in dilute solutions stable for a few days
-
bovine serum albumin required to prevent inactivation
hemoglobin partially preserves the enzyme activity of preparations during storage at -80°C
-
hemoglobin, 2 mg/ml, partially restores the activity of the enzyme stored at low concentrations, below 0.01 mg/ml
-
loss of activity after freezing and thawing
-
no stabilization by glycerol or His, ATP or unfractionated tRNA at concentrations similar to those in the enzyme assay
-
stability is reduced at protein concentrations of less than 0.015 mg/ml
-
stabilization of the isolated enzyme by hemoglobin and hematin
sulfhydryl groups are involved in the stabilization of the native structure of the enzyme
-