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6.1.1.21: histidine-tRNA ligase

This is an abbreviated version!
For detailed information about histidine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.21

Reaction

ATP
+
L-histidine
 +
tRNAHis
=
AMP
 +
diphosphate
 +
L-histidyl-tRNAHis

Synonyms

antihistidyl-tRNA synthetase, class II histidyl-tRNA synthetase, HARS, HARS1, HARS2, HisRS, HisRS-1, HisRS-2, Histidine translase, Histidine--tRNA ligase, Histidine--tRNA ligase homolog, histidine-tRNA ligase, histidine-tRNA ligase homolog, histidyl tRNA synthetase, Histidyl-transfer ribonucleate synthetase, histidyl-transfer RNA synthetase, Histidyl-tRNA synthetase, HRS, HTS1, Jo-1, Jo-1 antigen, mitochondrial histidyl tRNA synthetase, Synthetase, histidyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.21 histidine-tRNA ligase

Crystallization

Crystallization on EC 6.1.1.21 - histidine-tRNA ligase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure analysis
crystal structure of enzyme-substrate complex with histidyl-tRNA synthetase, ATP, and the amino acid analog histidinyl
-
crystal structure of histidyl-tRNA synthetase complexed with histidyl-adenylate, at 2.6 A
-
vapor diffusion method, using 0.1 M Tris pH 8.5, 0.2 M MgCl2 and 25% (w/v) PEG 3350
purified recombinant apoenzyme at 8 mg/ml, sitting drop method, protein solution: 20 mM Tris, pH 7.5, 0.2 M NaCl, 1 mM EDTA, 1 mM DTT, plus equal volume of well solution: 0.1 M sodium citrate, pH 5.6, 0.2 M sodium/potassium tartrate, 1.8 M ammonium sulfate, several days at room temperature, X-ray diffraction structure determination at 2.7 A resolution, and analysis
crystal structure analysis
enzyme complexed with tRNAHis, L-histidine, and AMPPNP, hanging drop vapor diffusion method, using 5% (w/v) PEG 4000 and 0.1 M NaOAc (pH4.5)
enzyme in complex with histidine
-
HisRS and its complex with histidine and its cognate tRNAHis
-
the structure of histidyl-tRNA synthetase is determined to a resolution of 2.7 A
hanging drop vapor diffusion method, using 0.2 M lithium sulfate or ammonium sulfate, 26% (w/v) PEG 3350, 0.1 M bis-Tris pH 5.5, 1 mM TCEP
the structure of histidyl-tRNA synthetase in complex with L-histidine is determined to a resolution of 1.8 A , in complex with histidyl-adenosinemonophosphate to a resolution of 3.05 A