6.1.1.21: histidine-tRNA ligase

This is an abbreviated version!
For detailed information about histidine-tRNA ligase, go to the full flat file.

Reaction

Synonyms

antihistidyl-tRNA synthetase, class II histidyl-tRNA synthetase, HARS, HARS1, HARS2, HisRS, HisRS-1, HisRS-2, Histidine translase, Histidine--tRNA ligase, Histidine--tRNA ligase homolog, histidine-tRNA ligase, histidine-tRNA ligase homolog, histidyl tRNA synthetase, Histidyl-transfer ribonucleate synthetase, histidyl-transfer RNA synthetase, Histidyl-tRNA synthetase, HRS, HTS1, Jo-1, Jo-1 antigen, mitochondrial histidyl tRNA synthetase, Synthetase, histidyl-transfer ribonucleate

ECTree

     6 Ligases

         6.1 Forming carbon-oxygen bonds

             6.1.1 Ligases forming aminoacyl-tRNA and related compounds

                6.1.1.21 histidine-tRNA ligase

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Results	in table
2	Activating Compound
49530	AA Sequence
4	Application
1	CAS Registry Number
22	Cloned(Commentary)
36	Cofactor
13	Crystallization (Commentary)
260	Disease/ Diagnostics
1	Expression
14	General Information
12	General Stability
7	IC50 Value
39	Inhibitors
14	kcat/KM [mM/s]
78	KM Value [mM]
22	Localization
19	Metals/Ions
41	Molecular Weight [Da]
63	Natural Substrates/ Products (Substrates)
111	Organism
1	Oxidation Stability
4	Pathway
86	PDB ID
12	pH Optimum
2	pH Range
1	Posttranslational Modification
57	Protein Variants
26	Purification (Commentary)
12	Reaction
62	Reaction Type
101	Reference
1	Renatured (Commentary)
29	Source Tissue
9	Specific Activity [micromol/min/mg]
5	Storage Stability
134	Substrates and Products (Substrate)
36	Subunits
346	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
61	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.17 - 130

ATP

10 entries

0.1 - 142

His

7 entries

0.39 - 130

L-histidine

6 entries

0.0013 - 0.024

RNA microhelix

2 entries

-

0.002 - 0.024

synthetic tRNAHis A-1

4 entries

-

0.11 - 6.08

synthetic tRNAHis G-1

5 entries

-

0.0004 - 5.4

tRNAHis

13 entries

0.26 - 1.5

tRNAHisCUA

2 entries

-

0.005 - 0.016

U73tRNAHisGUG

2 entries

-

0.64 - 2.6

wild type tRNAHis

2 entries

-

0.12 - 4

wild-type full length tRNAHis G-1

7 entries

-

additional information

additional information

Oryctolagus cuniculus

-

-

373

-

0.17

ATP

Escherichia coli

-

truncated enzyme form NcatHisRS, ATP-diphosphate exchange

361

0.2

ATP

Escherichia coli

-

mutant enzyme form des(A2-G10)-HisRS, ATP-diphosphate exchange

360

0.4

ATP

Escherichia coli

-

mutant enzyme form V-HisRS-R259Q, ATP-diphosphate exchange

360

0.48

ATP

Homo sapiens

-

mutant enzyme Y330C, at pH 7.5 and 25°C

745058

5.8

ATP

Homo sapiens

-

wild type enzyme, at pH 7.5 and 25°C

745058

8

ATP

Escherichia coli

-

enzyme form with N-terminal addition of valine V-HisRS, mutant enzyme form des(A2-Q6)-HisRS, ATP-diphosphate exchange

360

34

ATP

Escherichia coli

-

HisRS, ATP-diphosphate exchange

360

40

ATP

Saccharomyces cerevisiae

-

pH 8.0, 37°C, diphosphate exchange

677092

120

ATP

Escherichia coli

-

wild-type HisRS, ATP-diphosphate exchange

361

130

ATP

Escherichia coli

-

pH 8.0, 37°C, diphosphate exchange

677092

0.1

His

Escherichia coli

-

mutant enzyme form V-HisRS-R259K, aminoacylation

360

0.19

His

Escherichia coli

-

truncated enzyme form NcatHisRS, ATP-diphosphate exchange

361

1

His

Escherichia coli

-

mutant enzyme form des(A2-G10)-HisRS, aminoacylation

360

6

His

Escherichia coli

-

enzyme form with N-terminal addition of methionine M-HisRS, enzyme form with N-terminal addition of valineV-HisRS, aminoacylation

360

7

His

Escherichia coli

-

wild-type HisRS, aminoacylation

360

9

His

Escherichia coli

-

mutant enzyme form des(A2-Q6)-HisRS, aminoacylation, ATP, and mutant enzyme form with N-terminal addition of methionine M-HisRS, ATP-diphosphate exchange

360

142

His

Escherichia coli

-

wild-type HisRS, ATP-diphosphate exchange

361

0.39

L-histidine

Homo sapiens

-

mutant enzyme S365N, at pH 7.5 and 25°C

745058

0.74

L-histidine

Homo sapiens

-

mutant enzyme Y330C, at pH 7.5 and 25°C

745058

2.08

L-histidine

Homo sapiens

-

mutant enzyme V155G, at pH 7.5 and 25°C

745058

4.1

L-histidine

Homo sapiens

-

wild type enzyme, at pH 7.5 and 25°C

745058

40

L-histidine

Saccharomyces cerevisiae

-

pH 8.0, 37°C, diphosphate exchange

677092

130

L-histidine

Escherichia coli

-

pH 8.0, 37°C, diphosphate exchange

677092

0.0013

RNA microhelix

Saccharomyces cerevisiae

-

pH 8.0, 37°C, aminoacylation

677092

-

0.024

RNA microhelix

Escherichia coli

-

pH 8.0, 37°C, aminoacylation

677092

-

0.002

synthetic tRNAHis A-1

Escherichia coli

-

7.5, 37°C, enzyme mutant R123A

650256

-

0.012

synthetic tRNAHis A-1

Escherichia coli

-

7.5, 37°C, enzyme mutant R116A

650256

-

0.018

synthetic tRNAHis A-1

Escherichia coli

-

7.5, 37°C, wild-type enzyme

650256

-

0.024

synthetic tRNAHis A-1

Escherichia coli

-

7.5, 37°C, enzyme mutant R9H

650256

-

0.11

synthetic tRNAHis G-1

Escherichia coli

-

7.5, 37°C, enzyme mutant R123A

650256

-

0.13

synthetic tRNAHis G-1

Escherichia coli

-

7.5, 37°C, enzyme mutant R116A

650256

-

0.76

synthetic tRNAHis G-1

Escherichia coli

-

7.5, 37°C, enzyme mutant R9H

650256

-

4.75

synthetic tRNAHis G-1

Escherichia coli

-

7.5, 37°C, wild-type enzyme

650256

-

6.08

synthetic tRNAHis G-1

Escherichia coli

-

7.5, 37°C, enzyme mutant R9H

650256

-

0.0004

tRNAHis

Escherichia coli

P60906

mutant E83A, pH 7.5, 37°C

661192

0.0006

tRNAHis

Escherichia coli

P60906

mutant R159H, pH 7.5, 37°C

661192

0.01

tRNAHis

Escherichia coli

P60906

mutant E83Q, pH 7.5, 37°C

661192

0.048

tRNAHis

Escherichia coli

P60906

mutant Q127A, pH 7.5, 37°C

661192

0.3

tRNAHis

Homo sapiens

-

mutant enzyme S365N, at pH 7.5 and 25°C

745058

0.417

tRNAHis

Saccharomyces cerevisiae

-

pH 7.2, 37°C, wild-type substrate

716387

0.42

tRNAHis

Saccharomyces cerevisiae

-

pH 8.0, 37°C, aminoacylation

677092

0.56

tRNAHis

Homo sapiens

-

mutant enzyme Y330C, at pH 7.5 and 25°C

745058

1.71

tRNAHis

Escherichia coli

-

pH 8.0, 37°C, aminoacylation

677092

2.02

tRNAHis

Escherichia coli

P60906

wild-type, pH 7.5, 37°C

661192

3.05

tRNAHis

Homo sapiens

-

mutant enzyme V155G, at pH 7.5 and 25°C

745058

4

tRNAHis

Caulobacter vibrioides

-

wild-type Caulobacter crescentus tRNAHis, pH 7.5, 30°C

716383

5.4

tRNAHis

Homo sapiens

-

wild type enzyme, at pH 7.5 and 25°C

745058

0.26

tRNAHisCUA

Escherichia coli

-

truncated enzyme form NcatHisRS, aminoacylation

361

-

1.5

tRNAHisCUA

Escherichia coli

-

full-length HisRS, aminoacylation

361

-

0.005

U73tRNAHisGUG

Escherichia coli

-

full-length HisRS, aminoacylation

361

-

0.016

U73tRNAHisGUG

Escherichia coli

-

truncated enzyme form NcatHisRS, aminoacylation

361

-

0.64

wild type tRNAHis

Escherichia coli

-

truncated enzyme form NcatHisRS, aminoacylation

361

-

2.6

wild type tRNAHis

Escherichia coli

-

full-length HisRS, aminoacylation

361

-

0.12

wild-type full length tRNAHis G-1

Escherichia coli

-

7.5, 37°C, enzyme mutant R123A

650256

-

1.76

wild-type full length tRNAHis G-1

Escherichia coli

-

7.5, 37°C, enzyme mutant R116A

650256

-

2.04

wild-type full length tRNAHis G-1

Escherichia coli

-

7.5, 37°C, wild-type enzyme

650256

-

2.5 - 4

wild-type full length tRNAHis G-1

Escherichia coli

-

7.5, 37°C, enzyme mutant R9H

650256

-

2.54

wild-type full length tRNAHis G-1

Escherichia coli

-

7.5, 37°C, enzyme mutant R9H

650256

-

2.94

wild-type full length tRNAHis G-1

Escherichia coli

-

7.5, 37°C, enzyme mutant R116A

650256

-

2.94

wild-type full length tRNAHis G-1

Escherichia coli

-

7.5, 37°C, wild-type enzyme

650256

-