6.1.1.21: histidine-tRNA ligase

This is an abbreviated version!
For detailed information about histidine-tRNA ligase, go to the full flat file.

Reaction

Synonyms

antihistidyl-tRNA synthetase, class II histidyl-tRNA synthetase, HARS, HARS1, HARS2, HisRS, HisRS-1, HisRS-2, Histidine translase, Histidine--tRNA ligase, Histidine--tRNA ligase homolog, histidine-tRNA ligase, histidine-tRNA ligase homolog, histidyl tRNA synthetase, Histidyl-transfer ribonucleate synthetase, histidyl-transfer RNA synthetase, Histidyl-tRNA synthetase, HRS, HTS1, Jo-1, Jo-1 antigen, mitochondrial histidyl tRNA synthetase, Synthetase, histidyl-transfer ribonucleate

ECTree

     6 Ligases

         6.1 Forming carbon-oxygen bonds

             6.1.1 Ligases forming aminoacyl-tRNA and related compounds

                6.1.1.21 histidine-tRNA ligase

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Results	in table
2	Activating Compound
49530	AA Sequence
4	Application
1	CAS Registry Number
22	Cloned(Commentary)
36	Cofactor
13	Crystallization (Commentary)
260	Disease/ Diagnostics
1	Expression
14	General Information
12	General Stability
7	IC50 Value
39	Inhibitors
14	kcat/KM [mM/s]
78	KM Value [mM]
22	Localization
19	Metals/Ions
41	Molecular Weight [Da]
63	Natural Substrates/ Products (Substrates)
111	Organism
1	Oxidation Stability
4	Pathway
86	PDB ID
12	pH Optimum
2	pH Range
1	Posttranslational Modification
57	Protein Variants
26	Purification (Commentary)
12	Reaction
62	Reaction Type
101	Reference
1	Renatured (Commentary)
29	Source Tissue
9	Specific Activity [micromol/min/mg]
5	Storage Stability
134	Substrates and Products (Substrate)
36	Subunits
346	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
61	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 6.1.1.21 - histidine-tRNA ligase

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

108200

Streptococcus dysgalactiae subsp. equisimilis

-

nondenaturing PAGE, active fusion HisRS

357

115000

Rattus norvegicus

-

gel filtration

381

120000

Homo sapiens

P12081, P49590

-

650438

122000

Oryctolagus cuniculus

-

sucrose density gradient centrifugation

373

125000

Neurospora crassa

-

gel filtration

374

153000

Bos taurus

-

gel filtration, cytoplasmic

92

154000

Bos taurus

-

gel filtration, mitochondrial

92

160000

Bos taurus

-

electrophoresis under nondenaturing conditions

379

40000

2 entries

41100

Mycobacterium tuberculosis

P9WFV5

x * 41100, amino acid sequence determination

650438

42500

Escherichia coli

-

2 * 42500, SDS-PAGE

369

46600

Synechococcus sp.

Q55267

x * 46600, amino acid sequence determination

650438

46900

Escherichia coli

P60906

2 * 46900, fully purified native enzyme

650438

47100

Thermus thermophilus

P56194

x * 47100, amino acid sequence determination

650438

47300

Mycoplasma pneumoniae

P75069

x * 47300, amino acid sequence determination

650438

47800

Streptococcus dysgalactiae subsp. equisimilis

P30053

x * 47800, amino acid sequence determination

650438

47932

Streptococcus dysgalactiae subsp. equisimilis

-

2 * 47932, calculation from nucleotide sequence, wild-type enzyme

363

48300

2 entries

48800

Mycobacterium leprae

P46696

x * 48800, amino acid sequence determination

650438

49200

Synechocystis sp.

Q55653

x * 49200, amino acid sequence determination

650438

49800

Porphyra purpurea

P51348

x * 49800, amino acid sequence determination

650438

50000

Rattus norvegicus

-

2 * 50000, SDS-PAGE

381

52300

Caulobacter vibrioides

-

x * 52300

716383

53700

Streptococcus dysgalactiae subsp. equisimilis

-

2 * 53700, SDS-PAGE with or without reduction

357

55000

Homo sapiens

P12081, P49590

2 * 55000, dimeric quarternary structure

650438

55300

Oryza sativa

P93422

x * 55300, amino acid sequence determination

650438

57400

2 entries

60000

Saccharomyces cerevisiae

P07263

x * 60000, amino acid sequence determination

650438

62500

Neurospora crassa

-

2 * 62500, SDS-PAGE

374

64000

2 entries

78000

Salmonella enterica subsp. enterica serovar Typhimurium

-

high speed equilibrium centrifugation

367

80000

Bos taurus

-

2 * 80000, SDS-PAGE

379

85000

2 entries

92000

Salmonella enterica subsp. enterica serovar Typhimurium

-

gel filtration

367

94000

Streptococcus dysgalactiae subsp. equisimilis

-

gel filtration, active fusion HisRS

357

96000

Rattus norvegicus

-

gel filtration, measurement of the sedimentation coefficient

381

40000

Salmonella enterica subsp. enterica serovar Typhimurium

-

2 * 40000, centrifugation on guanidine HCl, SDS-PAGE

367

40000

Salmonella enterica subsp. enterica serovar Typhimurium

O52765

2 * 40000, dimeric quarternary structure

650438

48300

Mycoplasma genitalium

P47281

x * 48300, amino acid sequence determination

650438

48300

Methanocaldococcus jannaschii

Q58406

x * 48300, amino acid sequence determination

650438

57400

Mus musculus

Q61035

x * 57400, amino acid sequence determination

650438

57400

Homo sapiens

P12081, P49590

x * 57400, amino acid sequence determination

650438

64000

Rattus norvegicus

-

2 * 64000, SDS-PAGE

377

64000

Oryctolagus cuniculus

-

2 * 64000, SDS-PAGE

373

85000

Escherichia coli

P60906

-

650438

85000

Escherichia coli

-

gel filtration

369