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4.2.1.75: uroporphyrinogen-III synthase

This is an abbreviated version!
For detailed information about uroporphyrinogen-III synthase, go to the full flat file.

Word Map on EC 4.2.1.75

Reaction

Hydroxymethylbilane
=
uroporphyrinogen III
 +
H2O

Synonyms

CobA/HemD, Hydroxymethylbilane hydrolyase [cyclizing], hydroxymethylbilane hydrolyase, cyclizing, Isomerase, uroporphyrinogen, Porphobilinogenase, Synthase, uroporphyrinogen III co-, U3S, URO synthase, uro'gen III synthase, URO-synthase, UROIIIS, Uroporphyrinogen III co-synthase, Uroporphyrinogen III cosynthase, Uroporphyrinogen III synthase, Uroporphyrinogen isomerase, Uroporphyrinogen-III cosynthase, Uroporphyrinogen-III cosynthetase, uroporphyrinogen-III-synthase, UROS

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.75 uroporphyrinogen-III synthase

General Stability

General Stability on EC 4.2.1.75 - uroporphyrinogen-III synthase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
10% glycerol stabilizes the purified recombinant enzyme in 20 mM sodium phosphate, pH 7.45, 100 mM NaCl, 2 mM DTT, and 0.1 mM EDTA at -80°C
a helical region in the molecule is essential to retain the kinetic stability of the folded conformation
activity does not change after several cycles of freeze-and thawing in potassium phosphate buffer, pH 7.4, containing DTT and glycerol
dithiothreitol stabilizes
-
enzyme reaction linear up to 30 min at 35¦C
-
human erythrocyte enzyme more stable than wheat germ enzyme
-
purification needs to be carried out as close to 0¦C as possible
-
recombinant enzyme in cell lysate is unstable, glycerol stabilizes