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4.2.1.75: uroporphyrinogen-III synthase

This is an abbreviated version!
For detailed information about uroporphyrinogen-III synthase, go to the full flat file.

Word Map on EC 4.2.1.75

Reaction

Hydroxymethylbilane
=
uroporphyrinogen III
+
H2O

Synonyms

CobA/HemD, Hydroxymethylbilane hydrolyase [cyclizing], hydroxymethylbilane hydrolyase, cyclizing, Isomerase, uroporphyrinogen, Porphobilinogenase, Synthase, uroporphyrinogen III co-, U3S, URO synthase, uro'gen III synthase, URO-synthase, UROIIIS, Uroporphyrinogen III co-synthase, Uroporphyrinogen III cosynthase, Uroporphyrinogen III synthase, Uroporphyrinogen isomerase, Uroporphyrinogen-III cosynthase, Uroporphyrinogen-III cosynthetase, uroporphyrinogen-III-synthase, UROS

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.75 uroporphyrinogen-III synthase

Purification

Purification on EC 4.2.1.75 - uroporphyrinogen-III synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
from overproducing strain
-
Ni-NTA column chromatography and Superdex 75 gel filtration
partial
-
recombinant
recombinant His-tagged enzyme from Escherichia coli
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration to homogeneity
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the tag is cleaved off by TEV, followed by gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography, anion exchange chromatography, and gel filtration
recombinant N-terminally His-tagged enzyme from Escherichia coli strain strain BL21(DE3) by ultrafiltration, nickel affinity and anion exchange chromatography, followed by gel filtration to homogeneity
recombinant wild-type UROIIIS and 25 point mutants in Escherichia coli to over 97% purity
-