EC Number |
General Stability |
Reference |
---|
4.2.1.75 | 10% glycerol stabilizes the purified recombinant enzyme in 20 mM sodium phosphate, pH 7.45, 100 mM NaCl, 2 mM DTT, and 0.1 mM EDTA at -80°C |
682774 |
4.2.1.75 | a helical region in the molecule is essential to retain the kinetic stability of the folded conformation |
696334 |
4.2.1.75 | activity does not change after several cycles of freeze-and thawing in potassium phosphate buffer, pH 7.4, containing DTT and glycerol |
662062 |
4.2.1.75 | dithiothreitol stabilizes |
5883 |
4.2.1.75 | enzyme reaction linear up to 30 min at 35¦C |
5882 |
4.2.1.75 | human erythrocyte enzyme more stable than wheat germ enzyme |
5883 |
4.2.1.75 | purification needs to be carried out as close to 0¦C as possible |
5888 |
4.2.1.75 | recombinant enzyme in cell lysate is unstable, glycerol stabilizes |
662062 |