4.2.1.75: uroporphyrinogen-III synthase

This is an abbreviated version!
For detailed information about uroporphyrinogen-III synthase, go to the full flat file.

Reaction

Synonyms

CobA/HemD, Hydroxymethylbilane hydrolyase [cyclizing], hydroxymethylbilane hydrolyase, cyclizing, Isomerase, uroporphyrinogen, Porphobilinogenase, Synthase, uroporphyrinogen III co-, U3S, URO synthase, uro'gen III synthase, URO-synthase, UROIIIS, Uroporphyrinogen III co-synthase, Uroporphyrinogen III cosynthase, Uroporphyrinogen III synthase, Uroporphyrinogen isomerase, Uroporphyrinogen-III cosynthase, Uroporphyrinogen-III cosynthetase, uroporphyrinogen-III-synthase, UROS

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.75 uroporphyrinogen-III synthase

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Results	in table
4	Activating Compound
21465	AA Sequence
1	CAS Registry Number
20	Cloned(Commentary)
3	Crystallization (Commentary)
319	Disease/ Diagnostics
3	Expression
14	General Information
8	General Stability
38	Inhibitors
4	kcat/KM [mM/s]
14	KM Value [mM]
4	Localization
13	Metals/Ions
26	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
51	Organism
3	Oxidation Stability
8	Pathway
14	PDB ID
16	pH Optimum
4	pH Range
2	Posttranslational Modification
118	Protein Variants
20	Purification (Commentary)
8	Reaction
3	Reaction Type
50	Reference
1	Renatured (Commentary)
22	Source Tissue
11	Specific Activity [micromol/min/mg]
6	Storage Stability
56	Substrates and Products (Substrate)
17	Subunits
65	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
1	Temperature Range [°C]
18	Temperature Stability [°C]
8	Turnover Number [1/s]

Crystallization

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Crystallization on EC 4.2.1.75 - uroporphyrinogen-III synthase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

5 mg/ml purified recombinant wild-type and selenomethionine-enzyme, sitting drop vapour diffusion method, 4°C, equal volumes of protein solution, containing 10 mM Tris-HCl, pH 7.5, 1 mM dithiothreitol, and reservoir solution, containing 20% 2-methyl-2,4-pentanediol, 100 mM MES, pH 6.0, X-ray diffraction structure determination and analysis at 1.85-2.1 A resolution

Homo sapiens

P10746

650984

purified wild-type recombinant His6-tagged U3S and selenomethionine-substituted derivative, 0.001 ml of 20 mg/ml protein in 20 mM Hepes, pH 7.2, 20 mM NaCl, is mixed with 0.001 ml of reservoir solution containing 20% PEG 3350, 0.2 M tri-Na citrate, and equilibrated against 0.1 ml of reservoir solution, about a month at 8°C and followed by microseeding method, X-ray diffraction structure determination and analysis at 2.5 A resolution, single wavelength anomalous dispersion method

Pseudomonas syringae

Q88B90

714059

purified recombinant enzyme, three apoenzyme forms, U3S1, U3S2, U3S3, and one enzyme-product complex form, vapor diffusion in sitting drop method, mixing 0.002 ml of protein with 0.002 ml of precipitant solution and equilibration over 1 mL of precipitant solution of different compositions, for U3S1, 1.2 M NaH2PO4, 0.8 M K2H2PO4, for U3S2, 21-24% PEG 8000, 0.2 M Mg-acetate, 0.1 M MES-HCl, pH 6.5, 0.2 M NaCl, and for U3S3, 18-24% PEG 8000, 0.1 M Tris-HCl, pH 8.5, 0.2 mM MgCl2, 4°C, 1-7 days, X-ray diffraction structure determination and analysis at 1.6-2.0 A resolution

Thermus thermophilus

Q72KM1

696286