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3.1.14.1: yeast ribonuclease

This is an abbreviated version!
For detailed information about yeast ribonuclease, go to the full flat file.

Word Map on EC 3.1.14.1

Reaction

exonucleolytic cleavage to nucleoside 3'-phosphates =

Synonyms

RNA-depolymerase, RNase, ribonuclease U4, Afl II ribonuclease, RNase Trv, Gamma-toxin, RNA-degrading enzyme, nuclease 1, yeast mitochondrial degradosome, mtEXO, tRNA endonuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.14 Exoribonucleases producing 3'-phosphomonoesters
                3.1.14.1 yeast ribonuclease

Substrates Products

Substrates Products on EC 3.1.14.1 - yeast ribonuclease

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA + H2O
deoxynucleoside 3'-phosphates
show the reaction diagram
-
pUC18 or pSP1
-
?
polyadenylic acid + H2O
3'-AMP
show the reaction diagram
Saccharomyces fragilis x Saccharomyces dobzhanski
-
-
-
?
polyuridylic acid + H2O
3'-UMP
show the reaction diagram
Saccharomyces fragilis x Saccharomyces dobzhanski
-
-
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
show the reaction diagram
RNA + H2O
nucleoside 3'-phosphate + ?
show the reaction diagram
-
in vitro transcribed tRNAGlu3 UUC, tRNAGIn UUG and tRNALys UUU are substrates, stable anticodon stem and the anticodon loop are the minimal requirements for cleavage, synthetic minihelix RNA corresponding to the anticodon stem loop of the natural substrate tRNAGlu3 mcm5s2UUC is cleaved at the same position as the natural substrate. In ASLGlu3 UUC, the nucleotides U34U35C36A37C38 are required for optimal gamma-toxin cleavage, whereas a purine at position 32 or a G in position 33 dramatically reduces the cleavage of the anticodon stem loop
-
-
?
RNA + H2O
nucleoside 3'-phosphates
show the reaction diagram
additional information
?
-