3.1.14.1: yeast ribonuclease
This is an abbreviated version!
For detailed information about yeast ribonuclease, go to the full flat file.
Word Map on EC 3.1.14.1
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3.1.14.1
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interstrand
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alpha-toxin
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exoribonuclease
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pre-trnas
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beta-toxin
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zymocin
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fancd2
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karyomegalic
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leukocidins
-
mcm5s2u
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5-methoxycarbonylmethyl-2-thiouridine
-
analysis
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nutrition
- 3.1.14.1
-
interstrand
- alpha-toxin
- exoribonuclease
- pre-trnas
- beta-toxin
-
zymocin
-
fancd2
-
karyomegalic
-
leukocidins
-
mcm5s2u
-
5-methoxycarbonylmethyl-2-thiouridine
- analysis
- nutrition
Reaction
exonucleolytic cleavage to nucleoside 3'-phosphates =
Synonyms
Afl II ribonuclease, Gamma-toxin, mtEXO, nuclease 1, ribonuclease U4, RNA-degrading enzyme, RNA-depolymerase, RNase, RNase Trv, tRNA endonuclease, yeast mitochondrial degradosome
ECTree
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Substrates Products
Substrates Products on EC 3.1.14.1 - yeast ribonuclease
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REACTION DIAGRAM
polyadenylic acid + H2O
3'-AMP
Saccharomyces fragilis x Saccharomyces dobzhanski
-
-
-
?
polyuridylic acid + H2O
3'-UMP
Saccharomyces fragilis x Saccharomyces dobzhanski
-
-
-
?
RNA + H2O
nucleoside 3'-phosphate + ?
-
in vitro transcribed tRNAGlu3 UUC, tRNAGIn UUG and tRNALys UUU are substrates, stable anticodon stem and the anticodon loop are the minimal requirements for cleavage, synthetic minihelix RNA corresponding to the anticodon stem loop of the natural substrate tRNAGlu3 mcm5s2UUC is cleaved at the same position as the natural substrate. In ASLGlu3 UUC, the nucleotides U34U35C36A37C38 are required for optimal gamma-toxin cleavage, whereas a purine at position 32 or a G in position 33 dramatically reduces the cleavage of the anticodon stem loop
-
-
?
3'-phosphomononucleotides
-
exonucleolytic cleavage
no base specificity, preference for 3'-AMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
Saccharomyces fragilis x Saccharomyces dobzhanski
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
no base specificity, 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP, nucleotide release including 2',3'-cyclic nucloetides in decreasing order 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP, preference for 3'-AMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP, 2',3' cyclic mononucleotides
?
nucleoside 3'-phosphates
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mitochondrial mRNA and rRNA
-
?
RNA + H2O
nucleoside 3'-phosphates
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total RNA from Schizosaccharomyces pombe
-
?
?
-
-
not: DNA, diphenylphosphate, 2',3'-cyclic nucleotides
-
-
?
additional information
?
-
-
enzyme is part of the RNA degrading enzyme complex, the degradosome, in mitochondria, consisting of a RNA helicase and a RNase activity, the former being essential for exoribonuclease activity of the complex, the degradosome is not involved in tRNA processing
-
?
additional information
?
-
-
enzyme is part of the RNA degrading enzyme complex in mitochondria, the degradosome, which is a central part of a mitochondrial RNA surveillance system responsible for degradation of aberrant and unprocessed RNAs
-
?
additional information
?
-
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degradation of mitochondrial RNA to oligonucleoside 3'-phosphates after autolysis of the cells
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-
?
additional information
?
-
-
the enzyme is responsible for RNA turnover and degradation in mitochondria, mechanism of the degradosome acting from 3' to 5', overview
-
-
?
additional information
?
-
-
degradation of mitochondrial RNA to oligonucleoside 3'-phosphates after autolysis of the cells, determination of product compositions at different conditions, overview
-
-
?
additional information
?
-
-
the enzyme is not essential for degradation of 26S and 17S rRNA
-
?
additional information
?
-
-
only slight degradation of denaturated DNA, p-nitrophenyl phosphate, bis(p-nitrophenyl)phosphate
-
-
?