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Literature summary for 3.1.14.1 extracted from

  • Blank, A.; Dekker, C.A.
    Ribonuclease U4. Novel phosphotransferases catalyzing exonucleolytic degradation of ribonucleic acid (1972), Biochemistry, 11, 3962-3970.
    View publication on PubMed
Search for more literature for 3.1.14.1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribonucleic acid + H2O Ustilago sphaerogena exonucleolytic cleavage 3'-phosphomononucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Ustilago sphaerogena
-
 separable components RNase U4A, U4B, U4C
-

Reaction

Reaction Comment Organism Reaction ID
exonucleolytic cleavage to nucleoside 3'-phosphates exonucleolytic cleavage Ustilago sphaerogena
a

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Ustilago sphaerogena
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information only slight degradation of denaturated DNA, p-nitrophenyl phosphate, bis(p-nitrophenyl)phosphate Ustilago sphaerogena ?
-
 ?
ribonucleic acid + H2O exonucleolytic cleavage Ustilago sphaerogena 3'-phosphomononucleotides
-
 ?
ribonucleic acid + H2O exonucleolytic cleavage Ustilago sphaerogena 3'-phosphomononucleotides 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP ?
ribonucleic acid + H2O exonucleolytic cleavage Ustilago sphaerogena 3'-phosphomononucleotides 2',3' cyclic mononucleotides ?

Synonyms

Synonyms Comment Organism
ribonuclease U4
-
 Ustilago sphaerogena

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 U4A: 7.0 Ustilago sphaerogena
6 7 U4C: 6.0 Ustilago sphaerogena
6 7 U4B: 6.5 Ustilago sphaerogena

pH Range

pH Minimum pH Maximum Comment Organism
5 8 0.04 M sodium phosphate buffer Ustilago sphaerogena