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Literature summary for 3.1.14.1 extracted from

  • Dziembowski, A.; Piwowarski, J.; Hoser, R.; Minczuk, M.; Dmochowska, A.; Siep, M.; van der Spek, H.; Grivell, L.; Stepien, P.P.
    The yeast degradosome. Its composition, interplay between RNA helicase and RNase activities and the role in mitochondrial RNA metabolism (2003), J. Biol. Chem., 278, 1603-1611.
    View publication on PubMed
Search for more literature for 3.1.14.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants in different yeast strains Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
Y814S point mutation in Dssp1, mutant strain is respiratory incompetent with very unstable mitochondrial genomes Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae enzyme is part of the RNA degrading enzyme complex in mitochondria, the degradosome, which is a central part of a mitochondrial RNA surveillance system responsible for degradation of aberrant and unprocessed RNAs ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant degradosome from Saccharomyces cerevisiae W303 strain derivatives Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
exonucleolytic cleavage to nucleoside 3'-phosphates conserved Tyr814 is involved in catalysis and is essential for enzyme Dssp1 function and mitochondrial biogenesis Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is part of the RNA degrading enzyme complex, the degradosome, in mitochondria, consisting of a RNA helicase and a RNase activity, the former being essential for exoribonuclease activity of the complex, the degradosome is not involved in tRNA processing Saccharomyces cerevisiae ?
-
 ?
additional information enzyme is part of the RNA degrading enzyme complex in mitochondria, the degradosome, which is a central part of a mitochondrial RNA surveillance system responsible for degradation of aberrant and unprocessed RNAs Saccharomyces cerevisiae ?
-
 ?
RNA + H2O mitochondrial mRNA and rRNA Saccharomyces cerevisiae nucleoside 3'-phosphates
-
 ?

Subunits

Subunits Comment Organism
More enzyme is part of the RNA degrading enzyme complex, the degradosome, in mitochondria, consisting of 2 large subunits Dssp1 and Suv3p, a RNA helicase and a RNase activity, the former being essential for exoribonuclease activity of the complex Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
mtEXO
-
 Saccharomyces cerevisiae
yeast mitochondrial degradosome
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 RNA helicase assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 RNA helicase assay at Saccharomyces cerevisiae