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Literature summary for 3.1.14.1 extracted from

  • Ohtaka, Y.; Uchida, K.; Sakai, T.
    Purification and properties of ribonuclease from yeast (1963), J. Biochem., 54, 322-327.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA slight activation Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 100% inhibition by 0.01 M CdCl2 Saccharomyces cerevisiae
Co2+ 100% inhibition by 0.01 M CoCl2 Saccharomyces cerevisiae
Cu2+ 50% inhibition by 1 mM CuSO4 Saccharomyces cerevisiae
glutathione 95% inhibition by 0.01 M Saccharomyces cerevisiae
Mn2+ 20% of maximal activity Saccharomyces cerevisiae
Zn2+ 100% inhibition by 0.1 mM ZnSO4, reversible by addition of 1 mM EDTA, sodium citrate and histidine, not by glycine, arginine, agmatine and sodium acetate even at 0.01 M Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl slight activation Saccharomyces cerevisiae
KCl slight activation Saccharomyces cerevisiae
Na2SO4 slight activation Saccharomyces cerevisiae
KH2PO4 10-80% activation at various concentrations Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribonucleic acid + H2O Saccharomyces cerevisiae exonucleolytic cleavage 3'-phosphomononucleotides
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
additional information released from pressed cells Saccharomyces cerevisiae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
4°C, under toluene, pH 7.5, several weeks, little loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: DNA, diphenylphosphate, 2',3'-cyclic nucleotides Saccharomyces cerevisiae ?
-
?
ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces cerevisiae 3'-phosphomononucleotides
-
?
ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces cerevisiae 3'-phosphomononucleotides 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
30 min reaction time Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 65 less than 15% of maximum activity above and below Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
several h under toluene, pH 7.5 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9 Ph 4.5: about 15% of maximum activity, pH 9.0: about 50% of maximum activity Saccharomyces cerevisiae