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Literature summary for 3.1.14.1 extracted from

  • Lu, J.; Esberg, A.; Huang, B.; Bystroem, A.S.
    Kluyveromyces lactis gamma-toxin, a ribonuclease that recognizes the anticodon stem loop of tRNA (2008), Nucleic Acids Res., 36, 1072-1080.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.14.1

Activating Compound

Activating Compound Comment Organism Structure
additional information strong stimulatory effects of the mcm5 group, weak positive effect of the s2 group and a negative effect of the bacterial 5-methylaminomethyl group Kluyveromyces lactis
Trimethylamine N-oxide enhances cleavage of unmodified tRNA and anticodon stem loops by gamma-toxin-GST Kluyveromyces lactis

Cloned(Commentary)

Cloned (Comment) Organism
gamma-toxin-GST expressed in Escherichia coli BL21(DE3) Kluyveromyces lactis

Organism

Organism UniProt Comment Textmining
Kluyveromyces lactis
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-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Kluyveromyces lactis

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, stable for 12 months Kluyveromyces lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA + H2O in vitro transcribed tRNAGlu3 UUC, tRNAGIn UUG and tRNALys UUU are substrates, stable anticodon stem and the anticodon loop are the minimal requirements for cleavage, synthetic minihelix RNA corresponding to the anticodon stem loop of the natural substrate tRNAGlu3 mcm5s2UUC is cleaved at the same position as the natural substrate. In ASLGlu3 UUC, the nucleotides U34U35C36A37C38 are required for optimal gamma-toxin cleavage, whereas a purine at position 32 or a G in position 33 dramatically reduces the cleavage of the anticodon stem loop Kluyveromyces lactis nucleoside 3'-phosphate + ?
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 ?

Synonyms

Synonyms Comment Organism
Gamma-toxin
-
 Kluyveromyces lactis
tRNA endonuclease
-
 Kluyveromyces lactis