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3.1.14.1: yeast ribonuclease

This is an abbreviated version!
For detailed information about yeast ribonuclease, go to the full flat file.

Word Map on EC 3.1.14.1

Reaction

exonucleolytic cleavage to nucleoside 3'-phosphates =

Synonyms

RNA-depolymerase, RNase, ribonuclease U4, Afl II ribonuclease, RNase Trv, Gamma-toxin, RNA-degrading enzyme, nuclease 1, yeast mitochondrial degradosome, mtEXO, tRNA endonuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.14 Exoribonucleases producing 3'-phosphomonoesters
                3.1.14.1 yeast ribonuclease

Temperature Stability

Temperature Stability on EC 3.1.14.1 - yeast ribonuclease

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
several h under toluene, pH 7.5
50
-
10 min, less than 10% loss of activity
60
-
remaining activity after heating at 60°C for 90 min at pH 5.4 30%, at pH 7.0 40%, at pH 9.0 50%
70
-
2 min, complete inactivation
80
-
almost no activity
additional information
-
NaCl and KCl might protect from heat denaturation at 52°C