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3.1.14.1: yeast ribonuclease

This is an abbreviated version!
For detailed information about yeast ribonuclease, go to the full flat file.

Word Map on EC 3.1.14.1

Reaction

exonucleolytic cleavage to nucleoside 3'-phosphates =

Synonyms

Afl II ribonuclease, Gamma-toxin, mtEXO, nuclease 1, ribonuclease U4, RNA-degrading enzyme, RNA-depolymerase, RNase, RNase Trv, tRNA endonuclease, yeast mitochondrial degradosome

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.14 Exoribonucleases producing 3'-phosphomonoesters
                3.1.14.1 yeast ribonuclease

Inhibitors

Inhibitors on EC 3.1.14.1 - yeast ribonuclease

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,4-dithiothreitol
-
50% loss of activity at 5 mM, 75% at 10 mM
Ag+
-
no activity at 1 mM AgNO3
Ba2+
-
85% of maximal activity at 1 mM BaCl2
Ca2+
-
77% of activity at 1 mM CaCl2, 73% at 10 mM
EDTA
-
recoverage by either Mg2+, Cl-, or CaCl2
Fe2+
-
76% of activity at 1 mM FeCl2, 46% at 10 mM
Fe3+
-
41% of maximal activity at 1 mM FeCl3
glutathione
-
95% inhibition by 0.01 M
Hg2+
-
no activity at 1 mM HgCl2
Mg2+
-
91% of activity at 1 mM MgCl2, 77% at 10 mM
N-bromosuccinimide
-
-
Polyvinyl sulfate
Saccharomyces fragilis x Saccharomyces dobzhanski
-
70% of maximal activity with 50 microg/ml, 10% of maximal activity with 100 microg/ml
spermidine
Saccharomyces fragilis x Saccharomyces dobzhanski
-
slight inhibition: 80% of maximal activity with 0.01 M
additional information
-
tryptophan residues and disulfide bonds required for activity
-