3.1.1.14: chlorophyllase
This is an abbreviated version!
For detailed information about chlorophyllase, go to the full flat file.
Word Map on EC 3.1.1.14
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3.1.1.14
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phytol
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pheophorbide
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mg-dechelatase
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dephytylation
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pheophytinase
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bacteriochlorophyll
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stay-green
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bacteriochlorophyllide
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mg-free
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synthesis
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agriculture
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biotechnology
- 3.1.1.14
- phytol
- pheophorbide
- mg-dechelatase
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dephytylation
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pheophytinase
- bacteriochlorophyll
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stay-green
- bacteriochlorophyllide
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mg-free
- synthesis
- agriculture
- biotechnology
Reaction
Synonyms
ADK38_11350, ADK43_31155, ADK78_03330, ADL21_28415, ADL28_40565, ADL32_23315, AJAP_22265, AMED_0735, AMTR_s00002p00270990, AtCLH1, AtCLH2, AW19_2954, BC342_33805, BE15_26615, BG04_602, BoCLH1, BOLC2T10484H, BOLC5T30519H, BV96_01378, BXO91_01455, BZ13_341, CACLH, cce_5094, Cha6605_2121, Chase 1, Chl-degrading enzyme, CHL1, Chlase, Chlase 2, CHlase F1, Chlase F2, Chlase1, Chlase2, chlorophyll chlorophyllido-hydrolase, chlorophyll chlorophyllido-hydrolyase, chlorophyll chlorophyllidohydrolase, chlorophyll-chlorophyllide hydrolase, chlorophyll-chlorophyllido hydrolase, chlorophyll-chlorophyllido-hydrolase, chlorophyll-chlorophyllidohydrolase, chlorophyllase, chlorophyllase 1, chlorophyllase 2, chlorophyllase family protein, Chlorophyllase multi-domain protein, chlorophyllase type 0, Chlorophyllase-1, chlorophyllase-2, chlorophyllase-like, chlorophyllase1, CJJ17_24065, CLH, CLH1, CLH2, CLH3, coronatine-induced protein, coronatine-induced protein 1, CrCLH1, Csa_2G222090, CSB93_3304, CyanoCLH, CYY8467 Chlase2, DDY18_06460, DF19_10980, DJ90_4464, DJ93_5833, DM39_4274, DM48_2363, DR71_1130, DV20_35655, ERS008472_03552, ERS008530_03671, F775_31213, FG87_12610, FM21_30765, GKIL_3644, H489_0111595, HMPREF9554_01030, IbChlase, JCGZ_02225, KALB_5797, LK07_10060, LL06_17325, LP52_09115, MAB_3744, Micau_5231, More, MTR_1g041385, Namu_1618, Nos7524_0754, O3I_013485, Os10g0419600, P354_07750, POPTR_010G082300, QR77_15265, SAMN05421833_13812, SAMN05660733_05076, Sare_3190, SAVERM_2604, SD37_17575, SD80_11705, SETIT_9G266700v2, SGM_2508, SL103_16385, SNA_20280, Theco_3404, TK50_20200, TRIUR3_22141, truncated chlorophyllase 2, TU94_29540, UK23_27830, VAB18032_29991, VIT_07s0151g00110, VZ95_07895, XU06_03985, Y717_00135, YC6258_05192, Z051_21130, ZEAMMB73_Zm00001d032926
ECTree
3.1.1.14 chlorophyllaseAdvanced search results
results (
results (Engineering
Engineering on EC 3.1.1.14 - chlorophyllase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
H226A
the mutation causes almost complete loss of Chlase activity
H239A
the mutation causes almost complete loss of Chlase activity
H247A
the mutation causes almost complete loss of Chlase activity
H66A
the mutation causes almost complete loss of Chlase activity
C234A
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site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme
C248A
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site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme
C282A
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site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme
D264N
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site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme
H100A
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site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme
H241A
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site-directed mutagenesis, about 90% reduced activity compared to the wild-type enzyme
H254Y
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site-directed mutagenesis, about 90% reduced activity compared to the wild-type enzyme
H81A
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site-directed mutagenesis, about 85% reduced activity compared to the wild-type enzyme
S167A
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site-directed mutagenesis, about 50% reduced activity compared to the wild-type enzyme
additional information
additional information
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gene ATHCOR1: construction of transgenic Arabidopsis thaliana plants overexpressing the enzyme via infection with Agrobacterium tumefaciens and transformation, sense and antisense orientation, the sense mutation changed the chlorophyll a to chlorophyll b ratio, overview, gene AtCLH2: cloning and analysis
additional information
construction of T-DNA insertion clh1 and clh2 single and double knockout lines, which are still able to degrade chlorophyll during senescence, phenotypes, overview
additional information
construction of T-DNA insertion clh1 and clh2 single and double knockout lines, which are still able to degrade chlorophyll during senescence, phenotypes, overview
additional information
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construction of T-DNA insertion clh1 and clh2 single and double knockout lines, which are still able to degrade chlorophyll during senescence, phenotypes, overview
additional information
inhibition of expression of AtCLH2 by RNA interference, transfection using Agrobacterium tumefaciens strain LBA4404, AtCLH2 RNAi plants show decreased contents of chlorophyllide without a substantial change in the total amount of the extractable chlorophyll and consequently presented lower chlorophyllide to chlorophyll ratios in their leaves, phenotype, overview
additional information
construction of clh1 and clh1/clh2 mutant plants as transfer DNA insertion lines. Methyl jasmonate promotes chlorophyll degradation in the clh mutants as well as in the wild-type, phenotypes, overview
additional information
construction of clh1 and clh1/clh2 mutant plants as transfer DNA insertion lines. Methyl jasmonate promotes chlorophyll degradation in the clh mutants as well as in the wild-type, phenotypes, overview
additional information
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development of micellar electrokinetic chromatography (MEKC) assay for the plant membrane enzyme chlorophyllase, evaluation of several different permanently and dynamically coated capillaries
additional information
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construction of clh1 and clh1/clh2 mutant plants as transfer DNA insertion lines. Methyl jasmonate promotes chlorophyll degradation in the clh mutants as well as in the wild-type, phenotypes, overview
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additional information
a chimeric construct with the antisense gene BoCLH1, driven by the CaMV 35S promoter and Nos-terminator and harboring the hygromycin resistance gene, is used for Agrobacterium tumefaciens-mediated transformation, effects of the antisense BoCLH1 gene on the postharvest senescence of broccoli, phenotype with slower postharvest yellowing during storage in the dark at 20°C, overview
additional information
a chimeric construct with the antisense gene BoCLH1, driven by the CaMV 35S promoter and Nos-terminator and harboring the hygromycin resistance gene, is used for Agrobacterium tumefaciens-mediated transformation, effects of the antisense BoCLH1 gene on the postharvest senescence of broccoli, phenotype with slower postharvest yellowing during storage in the dark at 20°C, overview
additional information
a chimeric construct with the antisense gene BoCLH1, driven by the CaMV 35S promoter and Nos-terminator and harboring the hygromycin resistance gene, is used for Agrobacterium tumefaciens-mediated transformation, effects of the antisense BoCLH1 gene on the postharvest senescence of broccoli, phenotype with slower postharvest yellowing during storage in the dark at 20°C, overview
additional information
no effects on yellowing by antisense construct of gene CHL2
additional information
no effects on yellowing by antisense construct of gene CHL2
additional information
no effects on yellowing by antisense construct of gene CHL2
additional information
no effects on yellowing by antisense constructs of gene CHL3
additional information
no effects on yellowing by antisense constructs of gene CHL3
additional information
no effects on yellowing by antisense constructs of gene CHL3
additional information
usage of a polystyrene divinylbenzene-based metal chelator to purify and immobilize recombinant poly(His)-tagged recombinant BoCLH1. Cu2+ exhibits the highest capacity and purification efficiency, therefore DIAION®CR11Cu(II) is the preferred resin for enzyme immobilization. Enzyme immobilized on His-Trap resin is about 2fold stabilized at 60°C and during 17 cycles of use compared to free enzyme. Enzyme immobilized on resin is about 2fold stabilized at 60°C and during 17 cycles of use compared to free enzyme
additional information
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usage of a polystyrene divinylbenzene-based metal chelator to purify and immobilize recombinant poly(His)-tagged recombinant BoCLH1. Cu2+ exhibits the highest capacity and purification efficiency, therefore DIAION®CR11Cu(II) is the preferred resin for enzyme immobilization. Enzyme immobilized on His-Trap resin is about 2fold stabilized at 60°C and during 17 cycles of use compared to free enzyme. Enzyme immobilized on resin is about 2fold stabilized at 60°C and during 17 cycles of use compared to free enzyme
additional information
expression of full-length citrus Chlase results in limited chlorophyll breakdown in tobacco protoplasts and no visible leaf phenotype in whole plants, whereas expression of a Chlase version lacking the N-terminal 21 amino acids, i.e. ChlaseDELTAN, which corresponds to the mature protein, leads to extensive chlorophyll breakdown in both tobacco protoplasts and squash leaves, mutant ChlaseDELTAN-expressing squash leaves display a dramatic chlorotic phenotype in plants grown under low-intensity light, whereas under natural light a lesion-mimic phenotype occurrs, which follows the accumulation of chlorophyllide, a photodynamic chlorophyll breakdown product, phenotypes, overview
additional information
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entrapping of the enzyme in tetramethoxysilane-based sol-gel in the presence of lipid, acetone is used to introduce the substrate-chlorophyll into aqueous reaction medium in solubilized form, the entrapped chlorophyllase is less sensitive toward the denaturing effect of acetone in the bulk phase than the free enzyme, sol-gel at wet-stage, diffusion coefficient and partition coefficient of chlorophyll in sol-gel, overview
additional information
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optimization of chlorophyllase-catalyzed hydrolysis of chlorophyll in monophasic organic solvent media, best in a mixture of hexane/2-octanone of 98.3:1.7 v/v, overview
