Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | the activity of entrapped chlorophyllase increased with increasing acetone concentration from 6.5 to 20%, however, further increase of acetone leads to a decrease in chlorophyllase activity. Free chlorophyllase reaches its highest activity at acetone concentration of 10% in a medium consisting of water, acetone, and canola oil, overview | Phaeodactylum tricornutum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | entrapping of the enzyme in tetramethoxysilane-based sol-gel in the presence of lipid, acetone is used to introduce the substrate-chlorophyll into aqueous reaction medium in solubilized form, the entrapped chlorophyllase is less sensitive toward the denaturing effect of acetone in the bulk phase than the free enzyme, sol-gel at wet-stage, diffusion coefficient and partition coefficient of chlorophyll in sol-gel, overview | Phaeodactylum tricornutum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
canola oil | presence of 10%, 20%, and 30% of canola oil decreased the activity of entrapped chlorophyllase by 60%, 75%, and 80%, respectively | Phaeodactylum tricornutum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state and non-steady-state kinetics of free enzyme and of enzyme trapped in a sol-gel medium, overview | Phaeodactylum tricornutum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast membrane | - |
Phaeodactylum tricornutum | 31969 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll + H2O | Phaeodactylum tricornutum | - |
phytol + chlorophyllide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaeodactylum tricornutum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of the catalytic performance of purified free enzyme and purified enzyme trapped in a sol-gel medium, overview | Phaeodactylum tricornutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll + H2O | - |
Phaeodactylum tricornutum | phytol + chlorophyllide | - |
? | |
chlorophyll + H2O | chlorophyll from Spinacia oleracea | Phaeodactylum tricornutum | phytol + chlorophyllide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
free enzyme and of enzyme trapped in a sol-gel medium | Phaeodactylum tricornutum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | - |
Phaeodactylum tricornutum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Phaeodactylum tricornutum |