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3.1.1.14: chlorophyllase

This is an abbreviated version!
For detailed information about chlorophyllase, go to the full flat file.

Word Map on EC 3.1.1.14

Reaction

Chlorophyll
+
H2O
=
phytol
 +
chlorophyllide

Synonyms

ADK38_11350, ADK43_31155, ADK78_03330, ADL21_28415, ADL28_40565, ADL32_23315, AJAP_22265, AMED_0735, AMTR_s00002p00270990, AtCLH1, AtCLH2, AW19_2954, BC342_33805, BE15_26615, BG04_602, BoCLH1, BOLC2T10484H, BOLC5T30519H, BV96_01378, BXO91_01455, BZ13_341, CACLH, cce_5094, Cha6605_2121, Chase 1, Chl-degrading enzyme, CHL1, Chlase, Chlase 2, CHlase F1, Chlase F2, Chlase1, Chlase2, chlorophyll chlorophyllido-hydrolase, chlorophyll chlorophyllido-hydrolyase, chlorophyll chlorophyllidohydrolase, chlorophyll-chlorophyllide hydrolase, chlorophyll-chlorophyllido hydrolase, chlorophyll-chlorophyllido-hydrolase, chlorophyll-chlorophyllidohydrolase, chlorophyllase, chlorophyllase 1, chlorophyllase 2, chlorophyllase family protein, Chlorophyllase multi-domain protein, chlorophyllase type 0, Chlorophyllase-1, chlorophyllase-2, chlorophyllase-like, chlorophyllase1, CJJ17_24065, CLH, CLH1, CLH2, CLH3, coronatine-induced protein, coronatine-induced protein 1, CrCLH1, Csa_2G222090, CSB93_3304, CyanoCLH, CYY8467 Chlase2, DDY18_06460, DF19_10980, DJ90_4464, DJ93_5833, DM39_4274, DM48_2363, DR71_1130, DV20_35655, ERS008472_03552, ERS008530_03671, F775_31213, FG87_12610, FM21_30765, GKIL_3644, H489_0111595, HMPREF9554_01030, IbChlase, JCGZ_02225, KALB_5797, LK07_10060, LL06_17325, LP52_09115, MAB_3744, Micau_5231, More, MTR_1g041385, Namu_1618, Nos7524_0754, O3I_013485, Os10g0419600, P354_07750, POPTR_010G082300, QR77_15265, SAMN05421833_13812, SAMN05660733_05076, Sare_3190, SAVERM_2604, SD37_17575, SD80_11705, SETIT_9G266700v2, SGM_2508, SL103_16385, SNA_20280, Theco_3404, TK50_20200, TRIUR3_22141, truncated chlorophyllase 2, TU94_29540, UK23_27830, VAB18032_29991, VIT_07s0151g00110, VZ95_07895, XU06_03985, Y717_00135, YC6258_05192, Z051_21130, ZEAMMB73_Zm00001d032926

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.14 chlorophyllase

Purification

Purification on EC 3.1.1.14 - chlorophyllase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoenzymes: type 1 chlorophyllase and type 2 chlorophyllase
-
ammonium sulfate precipitation and Sephadex G-200gel filtration
amylose resin affinity column chromatography and DEAE Sepharose column chromatography
cold acetone extraction and column chromatography
-
Ni2+-NTA column chromatography
-
no isoenzymes
-
partial
partially
-
protein-A agarose bead immunoprecipitation
-
recombinant C-terminally His-tagged enzyme BoCHL1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant enzymes in the membrane fraction from Nicotiana tabacum chloroplasts by ultracentrifugation
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, or by using a microtiter plate purification method with immobilization of the enzyme, overview
-
recombinant isozyme AtCLH2 from Escherichia coli strain BL21 (DE3)
recombinant maltose-binding fusion protein from Escherichia coli
-
recombinant T7-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography