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Literature summary for 3.1.1.14 extracted from
- The chlorophyllases AtCLH1 and AtCLH2 are not essential for senescence-related chlorophyll breakdown in Arabidopsis thaliana (2007), FEBS Lett., 581, 5517-5525.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene chl1, DNA and amino acid sequence determination and anaylsis, transient expression of isozyme AtCLH1 as GFP-tagged protein, e.g. in senescent mesophyll protoplasts, expression analysis | Arabidopsis thaliana |
| gene chl2, DNA and amino acid sequence determination and anaylsis, transient expression of isozyme AtCLH2 as GFP-tagged protein, e.g. in senescent mesophyll protoplasts, expression analysis | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of T-DNA insertion clh1 and clh2 single and double knockout lines, which are still able to degrade chlorophyll during senescence, phenotypes, overview | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic vesicle | cytoplasmic | Arabidopsis thaliana | 31410 | - |
| additional information | no localization in chloroplasts of isozyme AtCLH1 | Arabidopsis thaliana | - |
- |
| additional information | no localization in chloroplasts of isozyme AtCLH2 | Arabidopsis thaliana | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorophyll + H2O | Arabidopsis thaliana | isozyme AtCLH1 is not essential for chlorophyll breakdown during plant senescence, overview | phytol + chlorophyllide | - |
? |  |
| chlorophyll + H2O | Arabidopsis thaliana | isozyme AtCLH2 is not essential for chlorophyll breakdown during plant senescence, overview | phytol + chlorophyllide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | O22527 | isozyme AtCLH1; isozyme AtCLH1 | - |
| Arabidopsis thaliana | Q9M7I7 | isozyme AtCLH2; isozyme AtCLH2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | green, isozyme AtCLH1 expression analysis | Arabidopsis thaliana | - |
| leaf | green, isozyme AtCLH2 expression analysis | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorophyll + H2O | isozyme AtCLH1 is not essential for chlorophyll breakdown during plant senescence, overview | Arabidopsis thaliana | phytol + chlorophyllide | - |
? | |
| chlorophyll + H2O | isozyme AtCLH2 is not essential for chlorophyll breakdown during plant senescence, overview | Arabidopsis thaliana | phytol + chlorophyllide | - |
? | |
| chlorophyll + H2O | removal of the lipophilic phytol moiety of chlorophyll | Arabidopsis thaliana | phytol + chlorophyllide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtCLH1 | - |
Arabidopsis thaliana |
| AtCLH2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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