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3.1.1.14: chlorophyllase

This is an abbreviated version!
For detailed information about chlorophyllase, go to the full flat file.

Word Map on EC 3.1.1.14

Reaction

Chlorophyll
+
H2O
=
phytol
 +
chlorophyllide

Synonyms

ADK38_11350, ADK43_31155, ADK78_03330, ADL21_28415, ADL28_40565, ADL32_23315, AJAP_22265, AMED_0735, AMTR_s00002p00270990, AtCLH1, AtCLH2, AW19_2954, BC342_33805, BE15_26615, BG04_602, BoCLH1, BOLC2T10484H, BOLC5T30519H, BV96_01378, BXO91_01455, BZ13_341, CACLH, cce_5094, Cha6605_2121, Chase 1, Chl-degrading enzyme, CHL1, Chlase, Chlase 2, CHlase F1, Chlase F2, Chlase1, Chlase2, chlorophyll chlorophyllido-hydrolase, chlorophyll chlorophyllido-hydrolyase, chlorophyll chlorophyllidohydrolase, chlorophyll-chlorophyllide hydrolase, chlorophyll-chlorophyllido hydrolase, chlorophyll-chlorophyllido-hydrolase, chlorophyll-chlorophyllidohydrolase, chlorophyllase, chlorophyllase 1, chlorophyllase 2, chlorophyllase family protein, Chlorophyllase multi-domain protein, chlorophyllase type 0, Chlorophyllase-1, chlorophyllase-2, chlorophyllase-like, chlorophyllase1, CJJ17_24065, CLH, CLH1, CLH2, CLH3, coronatine-induced protein, coronatine-induced protein 1, CrCLH1, Csa_2G222090, CSB93_3304, CyanoCLH, CYY8467 Chlase2, DDY18_06460, DF19_10980, DJ90_4464, DJ93_5833, DM39_4274, DM48_2363, DR71_1130, DV20_35655, ERS008472_03552, ERS008530_03671, F775_31213, FG87_12610, FM21_30765, GKIL_3644, H489_0111595, HMPREF9554_01030, IbChlase, JCGZ_02225, KALB_5797, LK07_10060, LL06_17325, LP52_09115, MAB_3744, Micau_5231, More, MTR_1g041385, Namu_1618, Nos7524_0754, O3I_013485, Os10g0419600, P354_07750, POPTR_010G082300, QR77_15265, SAMN05421833_13812, SAMN05660733_05076, Sare_3190, SAVERM_2604, SD37_17575, SD80_11705, SETIT_9G266700v2, SGM_2508, SL103_16385, SNA_20280, Theco_3404, TK50_20200, TRIUR3_22141, truncated chlorophyllase 2, TU94_29540, UK23_27830, VAB18032_29991, VIT_07s0151g00110, VZ95_07895, XU06_03985, Y717_00135, YC6258_05192, Z051_21130, ZEAMMB73_Zm00001d032926

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.14 chlorophyllase

Cloned

Cloned on EC 3.1.1.14 - chlorophyllase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Chlase gene, DNA and amino acid sequence determination and anaylsis, expression of precursor full-length wild-type Chlase and of a mutant ChlaseDELTAN lacking the N-terminal 21 amino acids, which corresponds to the mature enzyme, in two heterologous plant systems: in Cucurbita pepo cv. Ma’ayan plants using a ZYMV-based viral vector infective clone system and inoculation of cotyledons, and transiently in Nicotiana tabacum cv. Samsun NN chloroplast membranes of protoplasts, expression of full-length and truncated enzyme versions as thioredoxin fusion proteins in Escherichia coli
DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, and phylogenetic analysis and tree, the isozymes from cultivars TN57, CN1927, and CYY8467 share 95% identity, and an eight-nucleotide sequence of Chlase in CYY8467 is missing due to a frame-shift mutation of the Chlase gene leading to early termination of gene translation
DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, and phylogenetic analysis and tree, the isozymes from cultivars TN57, CN1927, and CYY8467 share 95% identity, and an eight-nucleotide sequence of Chlase2 in CYY8467 is missing due to a frame-shift mutation of the Chlase gene leading to early termination of gene translation implying that a frame shift mutation of the Chlase gene has evolved in CYY8467
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosseta-Gami B (DE3) cells
expressed in Escherichia coli strain BL21(DE3)
expression as His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression in Escherichia coli
expression of antisense construct of gene CLH3 in broccoli plants using Agrobacterium tumefaciens-mediated transformation
expression of antisense constructs of gene CLH1 in broccoli plants using Agrobacterium tumefaciens-mediated transformation
expression of antisense constructs of gene CLH2 in broccoli plants using Agrobacterium tumefaciens-mediated transformation
expression of gene ATHCOR1 in Escherichia coli BL21 as maltose-binding protein fusion protein, cloning of the gene into an expression vector for transformation of Arabidopsis thaliana plants
-
expression of wild-type and mutant enzymes in Escherichia coli
-
gene ADK38_11350, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene ADK43_31155, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene ADK78_03330, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
-
gene ADL21_28415, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene ADL28_40565, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene ADL32_23315, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
A0A0X3WMQ3
gene AJAP_22265, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene AMED_0735, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene ATCLH1, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene AW19_2954, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
A0A0B6FPX1
gene BC342_33805, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene BE15_26615, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene BG04_602, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene BOLC2T10484H, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene BOLC5T30519H, functional recombinant expression of C-terminally His-tagged enzyme BoCHL1 in Escherichia coli strain BL21(DE3)
gene BV96_01378, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
A0A031JJ58
gene BXO91_01455, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
A0A1X0M486
gene BZ13_341, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene CACLH, sequence analysis, comparisons, and phylogenetic analysis, overview. Recombinant expression as insoluble protein in Escherichia coli
gene cce_5094, sequence comparisons, recombinant expression of the N-terminally T7-tagged enzyme (T7 tag sequence is MASMTGGQQMG)in Escherichia coli strain BL21(DE3)
gene Cha6605_2121, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene chl1, DNA and amino acid sequence determination and anaylsis, transient expression of isozyme AtCLH1 as GFP-tagged protein, e.g. in senescent mesophyll protoplasts, expression analysis
gene chl2, DNA and amino acid sequence determination and anaylsis, transient expression of isozyme AtCLH2 as GFP-tagged protein, e.g. in senescent mesophyll protoplasts, expression analysis
gene chl2, expression anaylsis, recombinant expression in Escherichia coli strain BL21 (DE3)
gene Chlase1, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene CJJ17_24065, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene CLH, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene CLH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant C-terminally GFP-tagged enzyme expression driven by a CAMV35S promoter is determined in chloroplasts of Arabidopsis thaliana mesophyll protoplasts (isolated from 3-4-week-old well-expanded leaves yet to flower), recombinant overexpression of soluble N-terminally T7-tagged enzyme in Escherichia coli strain BL21(DE3)
gene clh1, recombinant expression of YFP-tagged enzyme in Arabidopsis thaliana, the YFP-tagged enzyme is detected outside of chloroplasts in leaf protoplasts prepared from plants constitutively expressing CLH1-YFP
gene CLH1, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene Csa_2G222090, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene CSB93_3304, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene DDY18_06460, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene DF19_10980, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
-
gene DJ90_4464, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene DJ93_5833, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene DM39_4274, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene DM48_2363, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
-
gene DR71_1130, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene DV20_35655, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene ERS008472_03552, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene ERS008530_03671, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene F775_31213, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene F775_31213, sequence analysis, comparisons, and phylogenetic analysis, overview. Recombinant expression as soluble protein in Escherichia coli
gene FG87_12610, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene FM21_30765, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
-
gene GKIL_3644, sequence analysis, comparisons, and phylogenetic analysis, overview
gene H489_0111595, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene HMPREF9554_01030, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene JCGZ_02225, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene KALB_5797, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene LK07_10060, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene LL06_17325, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene LP52_09115, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene MAB_3744, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene Micau_5231, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene MTR_1g041385, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene Namu_1618, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene Nos7524_0754, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene P354_07750, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene QR77_15265, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene SAMN05660733_05076, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene Sare_3190, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene SAVERM_2604, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene SD37_17575, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene SD80_11705, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene SETIT_9G266700v2, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene SGM_2508, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene SL103_16385, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene SNA_20280, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene Theco_3404, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene TK50_20200, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene TRIUR3_22141, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene TU94_29540, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene UK23_27830, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene VAB18032_29991, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene VZ95_07895, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene XU06_03985, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
-
gene Y717_00135, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
gene YC6258_05192, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
gene Z051_21130, sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble and soluble protein in Escherichia coli
sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as insoluble protein
sequence analysis, comparisons, and phylogenetic analysis, overview. Expression as soluble protein
sequence analysis, comparisons, and phylogenetic analysis, overview. Recombinant expression as soluble protein in Escherichia coli