1.5.3.25: fructosyl amine oxidase (glucosone-forming)

This is an abbreviated version!
For detailed information about fructosyl amine oxidase (glucosone-forming), go to the full flat file.

Reaction

Synonyms

AgaE-like protein, amadoriase, amadoriase I, amadoriase II, FaoA, FAOAo1, FAOAo2, FAOD, FAOD-Ao1, FAOD-Ao2, FAOX, FAOX-C, FAOX-II, FrlB, fructosyl amine:oxygen oxidoreductase, fructosyl amino acid oxidase, GAOA

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.3 With oxygen as acceptor

                1.5.3.25 fructosyl amine oxidase (glucosone-forming)

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Results	in table
11	AA Sequence
2	Application
10	Cloned(Commentary)
6	Cofactor
6	Crystallization (Commentary)
2	Expression
4	General Information
7	Inhibitors
66	kcat/KM [mM/s]
100	KM Value [mM]
2	Localization
3	Metals/Ions
6	Molecular Weight [Da]
34	Organism
8	pH Optimum
3	pI Value
57	Protein Variants
2	Purification (Commentary)
3	Reaction
33	Reference
2	Specific Activity [micromol/min/mg]
78	Substrates and Products (Substrate)
11	Subunits
22	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
75	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 1.5.3.25 - fructosyl amine oxidase (glucosone-forming)

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

40.8

Aspergillus fumigatus

O42629

melting temperature, mutant H106S/G150S

765813

45

Ulocladium sp. JS-103

-

1 h, 24% residual activity for wild-type, 80% for mutants R94W, R94F, R94L

765116

50

Arthrobacter sp. 2-4-1

Q7M0I4

half-life of wild-type 2.9 min, of mutant T60A/A188G/M244L/N257S/L261M 45 min, respectively

763943

52.4

Aspergillus fumigatus

O42629

melting temperature, wild-type

765813

55.3

Aspergillus fumigatus

O42629

melting temperature, mutant S67C/P121C

765813

60.6

Aspergillus fumigatus

O42629

melting temperature, mutant D295C/K303C

765813