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Literature summary for 1.5.3.25 extracted from

  • Sakaue, R.; Kajiyama, N.
    Thermostabilization of bacterial fructosyl-amino acid oxidase by directed evolution (2003), Appl. Environ. Microbiol., 69, 139-145 .
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
T60A/A188G/M244L/N257S/L261M mutant with improved thermostability, stable at 45°C Arthrobacter sp. 2-4-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
fructosyl glycine mutant T60A/A188G/M244L/N257S/L261M, pH 8, 37°C Arthrobacter sp. 2-4-1
0.74
-
fructosyl glycine wild-type, pH 8, 37°C Arthrobacter sp. 2-4-1
1.5
-
fructosyl L-valine mutant T60A/A188G/M244L/N257S/L261M, pH 8, 37°C Arthrobacter sp. 2-4-1
1.6
-
fructosyl L-valine wild-type, pH 8, 37°C Arthrobacter sp. 2-4-1

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. 2-4-1 Q7M0I4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fructosyl glycine + O2 + H2O
-
Arthrobacter sp. 2-4-1 glucosone + glycine + H2O2
-
?
fructosyl L-valine + O2 + H2O
-
Arthrobacter sp. 2-4-1 glucosone + L-valine + H2O2
-
?

Synonyms

Synonyms Comment Organism
FAOX
-
Arthrobacter sp. 2-4-1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
wild-type Arthrobacter sp. 2-4-1
50
-
mutant T60A/A188G/M244L/N257S/L261M Arthrobacter sp. 2-4-1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life of wild-type 2.9 min, of mutant T60A/A188G/M244L/N257S/L261M 45 min, respectively Arthrobacter sp. 2-4-1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.8
-
fructosyl glycine mutant T60A/A188G/M244L/N257S/L261M, pH 8, 37°C Arthrobacter sp. 2-4-1
8
-
fructosyl glycine wild-type, pH 8, 37°C Arthrobacter sp. 2-4-1
9.7
-
fructosyl L-valine mutant T60A/A188G/M244L/N257S/L261M, pH 8, 37°C Arthrobacter sp. 2-4-1
10.6
-
fructosyl L-valine wild-type, pH 8, 37°C Arthrobacter sp. 2-4-1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.5
-
fructosyl L-valine mutant T60A/A188G/M244L/N257S/L261M, pH 8, 37°C Arthrobacter sp. 2-4-1
6.6
-
fructosyl L-valine wild-type, pH 8, 37°C Arthrobacter sp. 2-4-1
10.8
-
fructosyl glycine wild-type, pH 8, 37°C Arthrobacter sp. 2-4-1
15.6
-
fructosyl glycine mutant T60A/A188G/M244L/N257S/L261M, pH 8, 37°C Arthrobacter sp. 2-4-1