Protein Variants | Comment | Organism |
---|---|---|
T60A/A188G/M244L/N257S/L261M | mutant with improved thermostability, stable at 45°C | Arthrobacter sp. 2-4-1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
fructosyl glycine | mutant T60A/A188G/M244L/N257S/L261M, pH 8, 37°C | Arthrobacter sp. 2-4-1 | |
0.74 | - |
fructosyl glycine | wild-type, pH 8, 37°C | Arthrobacter sp. 2-4-1 | |
1.5 | - |
fructosyl L-valine | mutant T60A/A188G/M244L/N257S/L261M, pH 8, 37°C | Arthrobacter sp. 2-4-1 | |
1.6 | - |
fructosyl L-valine | wild-type, pH 8, 37°C | Arthrobacter sp. 2-4-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. 2-4-1 | Q7M0I4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fructosyl glycine + O2 + H2O | - |
Arthrobacter sp. 2-4-1 | glucosone + glycine + H2O2 | - |
? | |
fructosyl L-valine + O2 + H2O | - |
Arthrobacter sp. 2-4-1 | glucosone + L-valine + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAOX | - |
Arthrobacter sp. 2-4-1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
wild-type | Arthrobacter sp. 2-4-1 |
50 | - |
mutant T60A/A188G/M244L/N257S/L261M | Arthrobacter sp. 2-4-1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life of wild-type 2.9 min, of mutant T60A/A188G/M244L/N257S/L261M 45 min, respectively | Arthrobacter sp. 2-4-1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.8 | - |
fructosyl glycine | mutant T60A/A188G/M244L/N257S/L261M, pH 8, 37°C | Arthrobacter sp. 2-4-1 | |
8 | - |
fructosyl glycine | wild-type, pH 8, 37°C | Arthrobacter sp. 2-4-1 | |
9.7 | - |
fructosyl L-valine | mutant T60A/A188G/M244L/N257S/L261M, pH 8, 37°C | Arthrobacter sp. 2-4-1 | |
10.6 | - |
fructosyl L-valine | wild-type, pH 8, 37°C | Arthrobacter sp. 2-4-1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.5 | - |
fructosyl L-valine | mutant T60A/A188G/M244L/N257S/L261M, pH 8, 37°C | Arthrobacter sp. 2-4-1 | |
6.6 | - |
fructosyl L-valine | wild-type, pH 8, 37°C | Arthrobacter sp. 2-4-1 | |
10.8 | - |
fructosyl glycine | wild-type, pH 8, 37°C | Arthrobacter sp. 2-4-1 | |
15.6 | - |
fructosyl glycine | mutant T60A/A188G/M244L/N257S/L261M, pH 8, 37°C | Arthrobacter sp. 2-4-1 |