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Literature summary for 1.5.3.25 extracted from

  • Fujiwara, M.; Sumitani, J.; Koga, S.; Yoshioka, I.; Kouzuma, T.; Imamura, S.; Kawaguchi, T.; Arai, M.
    Alteration of substrate specificity of fructosyl-amino acid oxidase from Ulocladium sp. JS-103 (2006), J. Biosci. Bioeng., 102, 241-243 .
    View publication on PubMed
Search for more literature for 1.5.3.25

Protein Variants

Protein Variants Comment Organism
R94F mutation enhances substrate specificity toward fructosyl valine Ulocladium sp. JS-103
R94L mutation enhances substrate specificity toward fructosyl valine Ulocladium sp. JS-103
R94W specificity of mutant enzyme toward fructosyl valine is 14fold that of the wild-type enzyme Ulocladium sp. JS-103

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76
-
 fructosyl L-valine mutant R94W, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
1.01
-
 fructosyl L-valine wild-type, pH 7.5, temperature not specified in the publication Ulocladium sp. JS-103
1.32
-
 fructosyl L-valine mutant R94F, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
2.33
-
 fructosyl L-valine mutant R94L, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
3.63
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine wild-type, pH 7.5, temperature not specified in the publication Ulocladium sp. JS-103
7.2
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant R94L, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
13.4
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant R94W, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
29
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant R94F, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103

Organism

Organism UniProt Comment Textmining
Ulocladium sp. JS-103
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fructosyl L-valine + O2 + H2O
-
 Ulocladium sp. JS-103 glucosone + L-valine + H2O2
-
 ?
Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine + O2 + H2O
-
 Ulocladium sp. JS-103 glucosone + N-alpha-benzyloxycarbonyl-L-valine + H2O2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 wild-type Ulocladium sp. JS-103
40
-
 mutants R94W, R94F, R94L Ulocladium sp. JS-103

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 1 h, 24% residual activity for wild-type, 80% for mutants R94W, R94F, R94L Ulocladium sp. JS-103

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.48
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant R94W, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
6.6
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant R94F, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
8.54
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant R94L, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
18.4
-
 fructosyl L-valine mutant R94W, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
27.5
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine wild-type, pH 7.5, temperature not specified in the publication Ulocladium sp. JS-103
31.9
-
 fructosyl L-valine mutant R94F, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
57.7
-
 fructosyl L-valine mutant R94L, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
69.8
-
 fructosyl L-valine wild-type, pH 7.5, temperature not specified in the publication Ulocladium sp. JS-103

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 wild-type Ulocladium sp. JS-103
8
-
 mutants R94W, R94F, R94L Ulocladium sp. JS-103

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.19
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant R94W, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
0.23
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant R94F, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
1.19
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant R94L, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
7.58
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine wild-type, pH 7.5, temperature not specified in the publication Ulocladium sp. JS-103
24.1
-
 fructosyl L-valine mutant R94F, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
24.1
-
 fructosyl L-valine mutant R94W, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
24.7
-
 fructosyl L-valine mutant R94L, pH 8.0, temperature not specified in the publication Ulocladium sp. JS-103
68.9
-
 fructosyl L-valine wild-type, pH 7.5, temperature not specified in the publication Ulocladium sp. JS-103