1.11.1.19: dye decolorizing peroxidase

This is an abbreviated version, for detailed information about dye decolorizing peroxidase, go to the full flat file.

Reaction

Reactive Blue 5
+ 2 H2O2 =
Phthalate
+
2,2'-disulfonyl azobenzene
+
3-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]benzenesulfonate
+ 2 H2O

Synonyms

AnaPX, dye-decolorizing peroxidase, DyP, DyP I, DyP II, DyP-I, DyP-type peroxidase, DyP1, DyP2, DyP3, EfeB, LiP BA45, LiP-SN, manganese-independent peroxidase I, manganese-independent peroxidase II, TT1485, tyrA, YcdB, YfeX, YwbN

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.19 dye decolorizing peroxidase

Specific Activity

Specific Activity on EC 1.11.1.19 - dye decolorizing peroxidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.022
-
enzyme from supernatant, at pH 4.0 and 30°C
0.058
-
recombinant enzyme from crude extract, at 30°C, pH 3.2
0.086
-
with veratryl glycerol-beta-guaiacyl ether, at pH 3.0 and 50°C
0.13
-
with veratryl alcohol, at pH 3.0 and 50°C
0.2
-
mutant enzyme D171N, at 30°C, pH not specified in the publication
0.28
-
crude enzyme, in 25 mM citrate buffer (pH 3.2), at 30°C
0.8
T1SP91;
enzyme from lysate supernatant, at pH 7.0 and 70°C
1.8
-
DyP, using Reactive Black 5 as substrate, in 0.1 M sodium tartrate buffer (pH 3.0), at 30°C
3.08
-
after 140fold purification, at pH 4.0 and 30°C
4.2
-
DyP, using Reactive Red 33 as substrate, in 0.1 M sodium tartrate buffer (pH 3.0), at 30°C
5.6
recombinant enzyme from crude extract, using Reactive Blue 5 as substrate, pH 4.0-4.4, at 37°C
7.5
-
DyP, using Reactive Blue 114 as substrate, in 0.1 M sodium tartrate buffer (pH 3.0), at 30°C
11.55
-
with Reactive Blue 19, at pH 3.0 and 30°C
17.1
-
unpurified supernatant, at pH 3.5 and 30°C
17.65
-
with Reactive Black 5, at pH 3.0 and 30°C
17.8
T1SP91;
after 22.3fold purification at pH 7.0 and 70°C
22.4
-
culture supernatant, at 30°C, pH not specified in the publication
28.4
-
culture liquid, at pH 3.0 and 20°C
34
-
DyP, using guaiacol as substrate, in 0.1 M sodium tartrate buffer (pH 3.0), at 30°C
37.8
-
substrate 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate, pH 5.0, temperature not specified in the publication
54
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using syringaldehyde as substrate
57
-
after 210fold purification, in 25 mM citrate buffer (pH 3.2), at 30°C
66.8
-
with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), at pH 3.0 and 50°C
71
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using 2,6-dimethoxyphenol as substrate
204
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid as substrate
216
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using pyrogallol as substrate
230
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using guaiacol as substrate
261
-
15.3fold purified isozyme Dyp2, at pH 3.5 and 30°C
270
-
DyP, using Reactive Blue 19 as substrate, in 0.1 M sodium tartrate buffer (pH 3.0), at 30°C
283
recombinant enzyme after 50.5fold purification, using Reactive Blue 5 as substrate, pH 4.0-4.4, at 37°C
290
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using L-ascorbate as substrate
308
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using D-isoascorbate as substrate; recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using NADPH as substrate
331
-
after 14.8fold purification, at 30°C, pH not specified in the publication
340
-
DyP, using Reactive Blue 5 as substrate, in 0.1 M sodium tartrate buffer (pH 3.0), at 30°C
347.8
-
manganese-independent peroxidase II after 12.2fold purification, at pH 3.0 and 20°C
374
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using NADH as substrate
469
-
manganese-independent peroxidase I after 16.5fold purification, at pH 3.0 and 20°C
600
-
wild type enzyme, at 30°C, pH not specified in the publication
732
-
42.8fold purified isozyme Dyp3, at pH 3.5 and 30°C
834
-
recombinant enzyme after 14385fold purification, at 30°C, pH 3.2
1478
recombinant enzyme, in 50 mM citrate buffer (pH 4.0-4.4), at 37°C, using 4-aminoantipyrine as substrate