1.11.1.19: dye decolorizing peroxidase

This is an abbreviated version, for detailed information about dye decolorizing peroxidase, go to the full flat file.

Reaction

Reactive Blue 5
+ 2 H2O2 =
Phthalate
+
2,2'-disulfonyl azobenzene
+
3-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]benzenesulfonate
+ 2 H2O

Synonyms

AnaPX, dye-decolorizing peroxidase, DyP, DyP I, DyP II, DyP-I, DyP-type peroxidase, DyP1, DyP2, DyP3, EfeB, LiP BA45, LiP-SN, manganese-independent peroxidase I, manganese-independent peroxidase II, TT1485, tyrA, YcdB, YfeX, YwbN

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.19 dye decolorizing peroxidase

pH Stability

pH Stability on EC 1.11.1.19 - dye decolorizing peroxidase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 5
-
isozymes DyP1, DyP2 and DyP3 are completely inactivated at pH 2.5. Isozymes DyP1, DyP2 and DyP3 exhibit approximately 20% activity at pH 3.0. Isozymes DyP1 and Dyp2 show almost 100% activity at pH 3.5, isozyme DyP3 shows about 95% activity at pH 3.5. Isozyme DyP1 shows less than 50% activity at pH 4.0, isozymes DyP2 and Dyp3 show about 50% activity at pH 4.0. Isozymes Dyp1, DyP2 and Dyp3 show about 30% activity at pH 4.5 and about 20% activity at pH 5.0
712519
3 - 5
-
the enzyme maintains 50% of its original activity at pH 3.0 and 5.0 after incubation for 10 and 6 h at 40°C, respectively
742674
3.5 - 9.5
when maintained at 40°C for 20 min, the enzyme is stable at pH values between 3.5 and 9.5. The enzyme shows 20% relative activity at pH 3.0 and 10.0, 40% relative activity at pH 4.0, about 65% relative activity at pH 4.5 and 9.0, about 80% relative activity at pH 5.0, more than 90% relative activity at pH 6.0-8.0
695785
4 - 9
-
the enzyme shows more than 80% activity after 24 h at pH 4.0-9.0
743731
4
rapid loss of activity below
723971
5 - 10
the enzyme shows more than 80% activity after incubation at pH 5.0-10.0 for 1 h at 37°C
743647
6.5 - 7
30 min, stable
723971
7 - 10
-
the enzyme maintains 100% of its original activity at pH values between 7 and 10 after incubation for 48 h at 40°C
742684