Cloned (Comment) | Organism |
---|---|
expressed in Aspergillus oryzae strain M-2-3, no exogenous heme is necessary for the expression of DyP | Geotrichum candidum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the oxidation of 2,6-dimethoxyphenol and guaiacol by rDyP occurs without the addition of Mn2+, and no enhancement of activity by the addition of Mn21 is observed | Geotrichum candidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
recombinant enzyme, SDS-PAGE | Geotrichum candidum |
60000 | - |
native enzyme, SDS-PAGE | Geotrichum candidum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geotrichum candidum | - |
strain Dec 1 | - |
Purification (Comment) | Organism |
---|---|
quaternary aminoethyl-Toyopearl column chromatography and Mono-Q column chromatography | Geotrichum candidum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:dextrose-grown cell | - |
Geotrichum candidum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22.4 | - |
culture supernatant, at 30°C, pH not specified in the publication | Geotrichum candidum |
331 | - |
after 14.8fold purification, at 30°C, pH not specified in the publication | Geotrichum candidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dimethoxyphenol + H2O2 + H+ | - |
Geotrichum candidum | ? | - |
? | |
guaiacol + H2O2 + H+ | - |
Geotrichum candidum | ? | - |
? | |
additional information | veratryl alcohol, Reactive Red 123, Reactive Orange 13, and Reactive Yellow 2 are not decolorized by the recombinant enzyme | Geotrichum candidum | ? | - |
? | |
Reactive Black 5 + H2O2 + H+ | weak decolorization, 0.5% activity of the native wild type enzyme compared to Reactive Blue 5 | Geotrichum candidum | oxidized Reactive Black 5 + H2O | - |
? | |
Reactive Blue 19 + H2O2 + H+ | 92% activity of the native wild type enzyme compared to Reactive Blue 5 | Geotrichum candidum | oxidized Reactive Blue 19 + H2O | - |
? | |
Reactive Blue 5 + H2O2 + H+ | 100% activity | Geotrichum candidum | oxidized Reactive Blue 5 + H2O | - |
? | |
Reactive Red 120 + H2O2 + H+ | slight decolorization | Geotrichum candidum | oxidized Reactive Violet 23 + H2O | - |
? | |
Reactive Red 33 + H2O2 + H+ | weak decolorization, 2% activity of the native wild type enzyme compared to Reactive Blue 5 | Geotrichum candidum | oxidized Reactive Red 33 + H2O | - |
? | |
Reactive Violet 23 + H2O2 + H+ | weak decolorization | Geotrichum candidum | oxidized Reactive Violet 23 + H2O | - |
? | |
Reactive Yellow 2 + H2O2 + H+ | 2.6% activity of the native wild type enzyme compared to Reactive Blue 5 | Geotrichum candidum | oxidized Reactive Yellow 2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DyP | - |
Geotrichum candidum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | after 2 h heat treatment, the native enzyme shows 97% residual activity at 40°C, 93% residual activity at 50°C, and 79% residual activity at 60°C when measured at 30°C for 1 min. After heating at 30, 40, and 50°C for 120 min, recombinant DyP retains more than 90% of its activity when measured at 30°C for 1 min, but the enzyme gradually becomes inactive after treatment at 60°C (12% residual activity) and is rapidly inactivated at 70°C | Geotrichum candidum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | 3.2 | recombinant enzyme | Geotrichum candidum |
3.2 | - |
native enzyme | Geotrichum candidum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | DyP is a heme-containing peroxidase | Geotrichum candidum |