Activating Compound | Comment | Organism | Structure |
---|---|---|---|
H2O2 | the maximum activity is obtained with an initial H2O2 concentration of about 0.33 mM | Bjerkandera adusta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 55% residual activity at 1 mM; 70% residual activity at 1 mM | Bjerkandera adusta | |
Ag+ | 50% residual activity at 5 mM; 82% residual activity at 5 mM | Bjerkandera adusta | |
Ba2+ | 90% residual activity at 5 mM | Bjerkandera adusta | |
Cd2+ | complete inhibition at 5 mM | Bjerkandera adusta | |
Co2+ | 66% residual activity at 5 mM | Bjerkandera adusta | |
dithiothreitol | 65% residual activity at 1 mM; 81% residual activity at 1 mM | Bjerkandera adusta | |
Fe2+ | 85% residual activity at 5 mM | Bjerkandera adusta | |
Hg2+ | complete inhibition at 5 mM | Bjerkandera adusta | |
hydroxylamine | 10% residual activity at 2 mM; 25% residual activity at 2 mM | Bjerkandera adusta | |
L-cystine | 10% residual activity at 2 mM; 25% residual activity at 2 mM | Bjerkandera adusta | |
potassium cyanide | 3% residual activity at 10 mM; complete inhibition at 10 mM | Bjerkandera adusta | |
SDS | 27% inhibition at 20 mM; 68% residual activity at 20 mM; 73% residual activity at 20 mM | Bjerkandera adusta | |
Sodium azide | 5% residual activity at 10 mM; complete inhibition at 10 mM | Bjerkandera adusta | |
Zn2+ | 88% residual activity at 5 mM | Bjerkandera adusta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.099 | - |
2,4-Dichlorophenol | at pH 4.0 and 30°C | Bjerkandera adusta | |
0.099 | - |
2,4-Dichlorophenol | at pH 3.0 and 70°C | Bjerkandera adusta | |
0.151 | - |
2,6-dimethoxyphenol | at pH 4.0 and 50°C | Bjerkandera adusta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 118% activity at 5 mM | Bjerkandera adusta | |
Ca2+ | 110% activity at 5 mM | Bjerkandera adusta | |
Co2+ | 120% activity at 5 mM | Bjerkandera adusta | |
Cu2+ | 114% activity at 5 mM | Bjerkandera adusta | |
Cu2+ | 133% activity at 5 mM | Bjerkandera adusta | |
Fe2+ | 109% activity at 5 mM | Bjerkandera adusta | |
K+ | 125% activity at 5 mM | Bjerkandera adusta | |
Mg2+ | 180% activity at 5 mM | Bjerkandera adusta | |
Mg2+ | 180% activity at 5 mM. The addition of 5 mM of Mg2+ enhances the thermostability of the enzyme | Bjerkandera adusta | |
Mn2+ | 112% activity at 5 mM | Bjerkandera adusta | |
Mn2+ | 225% activity at 5 mM. The addition of 5 mM of Mn2+ enhances the thermostability of the enzyme | Bjerkandera adusta | |
additional information | not significantly affected by Mg2+, Ba2+, Li+, and K+ | Bjerkandera adusta | |
additional information | not significantly affected by Zn2+, Fe2+, and Li+ | Bjerkandera adusta | |
Zn2+ | 104% activity at 5 mM | Bjerkandera adusta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Reactive Blue 5 + H2O2 | Bjerkandera adusta | - |
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
hexane | the enzyme retains at least 22% of the activity of incubation in the presence of 30% (v/v) hexane | Bjerkandera adusta |
hexane | the enzyme retains at least 75% of the activity of incubation in the presence of 30% (v/v) hexane | Bjerkandera adusta |
isopropanol | the enzyme retains at least 115% of the activity of incubation in the presence of 30% (v/v) isopropanol | Bjerkandera adusta |
isopropanol | the enzyme retains at least 95% of the activity of incubation in the presence of 30% (v/v) isopropanol | Bjerkandera adusta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bjerkandera adusta | - |
- |
- |
Bjerkandera adusta CX-9 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation-dialysis, Mono-S Sepharose anion-exchange column chromatography, and Sephacryl S-200 gel filtration | Bjerkandera adusta |
ammonium sulfate precipitation-dialysis, Sepharose column chromatography, and Sephacryl S-200 gel filtration | Bjerkandera adusta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.12 | - |
after 48fold purification, with 2,6-dimethoxyphenol as substrate, at pH 3.0 and 70°C | Bjerkandera adusta |
18.6 | - |
after 60fold purification, with 2,6-dimethoxyphenol as substrate, at pH 4.0 and 50°C | Bjerkandera adusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | 65% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | 65% activity compared to 2,4-dichlorophenol | Bjerkandera adusta CX-9 | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | 65% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | 82% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O | - |
? | |
2,4,5-trichlorophenol + H2O2 + H+ | 31% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | ? | - |
? | |
2,4,5-trichlorophenol + H2O2 + H+ | 60% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized 2,4,5-trichlorophenol + | - |
? | |
2,4,5-trichlorophenol + H2O2 + H+ | 31% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | oxidized 2,4,5-trichlorophenol + H2O | - |
? | |
2,4,5-trichlorophenol + H2O2 + H+ | 64% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized 2,4,5-trichlorophenol + H2O | - |
? | |
2,4-dichlorophenol + H2O2 + H+ | 100% activity | Bjerkandera adusta | ? | - |
? | |
2,4-dichlorophenol + H2O2 + H+ | 100% activity with 2,4-dichlorophenol, 88% activity with H2O2 | Bjerkandera adusta | oxidized 2,4-dichlorophenol + H2O | - |
? | |
2,4-dichlorophenol + H2O2 + H+ | 70% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized 2,4-dichlorophenol + H2O | - |
? | |
2,6-dichlorophenol + H2O2 + H+ | 69% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | ? | - |
? | |
2,6-dichlorophenol + H2O2 + H+ | 69% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | oxidized 2,6-dichlorophenol + H2O | - |
? | |
2,6-dichlorophenol + H2O2 + H+ | 66% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized 2,6-dichlorophenol + H2O | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | 75% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | 75% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | oxidized 2,6-dimethoxyphenol + H2O | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | 100% activity with 2,6-dimethoxyphenol, 95% activity with H2O2 | Bjerkandera adusta | oxidized 2,6-dimethoxyphenol + H2O | - |
? | |
2-dianisidine + H2O2 + H+ | 85% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | oxidized 2-dianisidine + H2O | - |
? | |
2-dianisidine + H2O2 + H+ | 91% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized 2-dianisidine + H2O | - |
? | |
4-chlorophenol + H2O2 + H+ | 25% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized 4-chlorophenol + H2O | - |
? | |
Acid Blue 158 + H2O2 + H+ | - |
Bjerkandera adusta | oxidized Acid Blue 158 + H2O | - |
? | |
Acid Blue 158 + H2O2 + H+ | highest decolorization activity (about 90% after 12 h) | Bjerkandera adusta | oxidized Acid Blue 158 + H2O | - |
? | |
Cibacet Brilliant Blue BG + H2O2 + H+ | - |
Bjerkandera adusta | oxidized Cibacet Brilliant Blue BG + H2O | - |
? | |
commercial humic acid + H2O2 + H+ | 81% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | ? | - |
? | |
coniferyl alcohol + H2O2 + H+ | 97% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | ? | - |
? | |
coniferyl alcohol + H2O2 + H+ | 97% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | coniferaldehyde + H2O | - |
? | |
coniferyl alcohol + H2O2 + H+ | 65% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | coniferaldehyde + H2O | - |
? | |
guaiacol + H2O2 + H+ | 91% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | ? | - |
? | |
guaiacol + H2O2 + H+ | 55% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized guaiacol + H2O | - |
? | |
guaiacol + H2O2 + H+ | 91% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | oxidized guaicol + H2O | - |
? | |
humic acid + H2O2 + H+ | 81% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | oxidized humic acid + H2O | - |
? | |
humic acid + H2O2 + H+ | 75% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized humic acid + H2O | - |
? | |
Indigo Carmine + H2O2 + H+ | - |
Bjerkandera adusta | oxidized Indigo Carmine + H2O | - |
? | |
L-DOPA + H2O2 + H+ | 26% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | ? | - |
? | |
L-DOPA + H2O2 + H+ | 26% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | oxidized L-DOPA + H2O | - |
? | |
L-DOPA + H2O2 + H+ | 70% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized L-DOPA + H2O | - |
? | |
Methyl Green + H2O2 + H+ | - |
Bjerkandera adusta | oxidized Methyl Green + H2O | - |
? | |
additional information | no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol | Bjerkandera adusta | ? | - |
- |
|
additional information | no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol | Bjerkandera adusta | ? | - |
? | |
additional information | no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol | Bjerkandera adusta CX-9 | ? | - |
- |
|
additional information | no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol | Bjerkandera adusta CX-9 | ? | - |
? | |
o-dianisidine + H2O2 + H+ | 85% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | ? | - |
? | |
pentachlorophenol + H2O2 + H+ | 10% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized pentachlorophenol + H2O | - |
? | |
Poly R-478 + H2O2 + H+ | - |
Bjerkandera adusta | oxidized Poly R-478 + H2O | - |
? | |
pyrogallol + H2O2 + H+ | 80% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | ? | - |
? | |
pyrogallol + H2O2 + H+ | 80% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | oxidized pyrogallol + H2O | - |
? | |
pyrogallol + H2O2 + H+ | 52% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized pyrogallol + H2O | - |
? | |
Reactive Blue 5 + H2O2 | - |
Bjerkandera adusta | phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
Reactive Dye Remazol Brilliant Violet 5R + H2O2 + H+ | - |
Bjerkandera adusta | oxidized Reactive Dye Remazol Brilliant Violet 5R + H2O | - |
? | |
Remazol Brilliant Blue Reactif + H2O2 + H+ | - |
Bjerkandera adusta | oxidized Remazol Brilliant Blue Reactif + H2O | - |
? | |
Remazol Brilliant Blue Reactif + H2O2 + H+ | highest decolorization activity (about 90% after 12 h) | Bjerkandera adusta | oxidized Remazol Brilliant Blue Reactif + H2O | - |
? | |
signayl alcohol + H2O2 + H+ | 68% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | oxidized signaldehyde + H2O | - |
? | |
signayl alcohol + H2O2 + H+ | 94% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | signaldehyde + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | 99% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | veratraldehyde + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | 72% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta | veratraldehyde + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | 99% activity compared to 2,4-dichlorophenol | Bjerkandera adusta CX-9 | veratraldehyde + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | 72% activity compared to 2,6-dimethoxyphenol | Bjerkandera adusta CX-9 | veratraldehyde + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | 99% activity compared to 2,4-dichlorophenol | Bjerkandera adusta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Bjerkandera adusta |
monomer | 1 * 30000, SDS-PAGE | Bjerkandera adusta |
monomer | 1 * 45221, calculated from amino acid sequence | Bjerkandera adusta |
monomer | 1 * 30125, calculated from amino acid sequence | Bjerkandera adusta |
Synonyms | Comment | Organism |
---|---|---|
DyP | - |
Bjerkandera adusta |
LiP BA45 | - |
Bjerkandera adusta |
MnP BA30 | - |
Bjerkandera adusta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bjerkandera adusta |
70 | - |
- |
Bjerkandera adusta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the half-life times of the enzyme in the absence of 5 mM of Mg2+ are 6, 4, and 2 h at 40, 50, and 60°C, respectively | Bjerkandera adusta |
60 | - |
the half-life times of the enzyme in the absence of 5 mM of Mg2+ are 6, 4, and 2 h at 40, 50, and 60 °C, respectively. The half-life time of the enzyme at 40, 50, and 60°C increases to 7, 5, and 3 h in the presence 5 mM of Mg2+, respectively | Bjerkandera adusta |
60 | 80 | the half-life times of the enzyme in the absence of 5 mM of Mn2+ are 8, 5, and 2 h at 60, 70, and 80°C, respectively. The half-life time of the purified peroxidase at 60, 70, and 80°C increases to 10, 7, and 4 h in the presence 5 mM of MnSO4, respectively | Bjerkandera adusta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.101 | - |
2,4-Dichlorophenol | at pH 4.0 and 30°C | Bjerkandera adusta | |
0.101 | - |
2,4-Dichlorophenol | at pH 3.0 and 70°C | Bjerkandera adusta | |
0.207 | - |
2,6-dimethoxyphenol | at pH 4.0 and 50°C | Bjerkandera adusta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
- |
Bjerkandera adusta |
4 | - |
- |
Bjerkandera adusta |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 4 | the enzyme maintains 50% of its original activity at pH 2.0 and 4.0 after incubation for 9 and 5 h at 50°C, respectively | Bjerkandera adusta |
3 | 5 | the enzyme maintains 50% of its original activity at pH 3.0 and 5.0 after incubation for 10 and 6 h at 40°C, respectively | Bjerkandera adusta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Bjerkandera adusta |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
5 | - |
at pH 4.0 and 50°C | Bjerkandera adusta | Ag+ |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.02 | - |
2,4-Dichlorophenol | at pH 3.0 and 70°C | Bjerkandera adusta | |
1.0235 | - |
2,4-Dichlorophenol | at pH 4.0 and 30°C | Bjerkandera adusta | |
1.37 | - |
2,6-dimethoxyphenol | at pH 4.0 and 50°C | Bjerkandera adusta |