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Literature summary for 1.11.1.19 extracted from

  • Sugano, Y.; Muramatsu, R.; Ichiyanagi, A.; Sato, T.; Shoda, M.
    DyP, a unique dye-decolorizing peroxidase, represents a novel heme peroxidase family: Asp171 replaces the distal histidine of classical peroxidases (2007), J. Biol. Chem., 282, 36652-36658.
    View publication on PubMed
Search for more literature for 1.11.1.19

Application

Application Comment Organism
degradation DyP is a promising enzyme for the decolorizing treatment of dye-contaminated water Thanatephorus cucumeris

Crystallization (Commentary)

Crystallization (Comment) Organism
batch method, using 0.89 M ammonium sulfate, 0.92 M sodium chloride, at 10°C Thanatephorus cucumeris

Protein Variants

Protein Variants Comment Organism
D171N the mutant displays 3000fold less enzymatic activity compared to the wild type DyP Thanatephorus cucumeris

Metals/Ions

Metals/Ions Comment Organism Structure
Iron DyP contains one heme with an iron at the center of the molecule Thanatephorus cucumeris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 gel filtration Thanatephorus cucumeris

Organism

Organism UniProt Comment Textmining
Thanatephorus cucumeris M5C6U3 strain Dec 1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Thanatephorus cucumeris

Purification (Commentary)

Purification (Comment) Organism
-
 Thanatephorus cucumeris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 mutant enzyme D171N, at 30°C, pH not specified in the publication Thanatephorus cucumeris
600
-
 wild type enzyme, at 30°C, pH not specified in the publication Thanatephorus cucumeris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-amino-2-sulfonyl-4-aminomethyl-9,10-anthraquinone + H2O2 + H+
-
 Thanatephorus cucumeris ?
-
 ?

Synonyms

Synonyms Comment Organism
DyP
-
 Thanatephorus cucumeris

Cofactor

Cofactor Comment Organism Structure
heme DyP contains one heme with an iron at the center of the molecule Thanatephorus cucumeris