acts as a cofactor, it has a high Log D at pH 5.0. The increase in the activity of the enzyme with propanoic acid around 10-50°C is due to the peroxidation step because high activity in the nonenzymatic oxidative bromination step is maintained at low temperature, which suppresses the decomposition of the active species generated by the reaction between peracid and Br-
the carboxylic acids including hydroxyacetic acid, cyanoacetic acid, bromoacetic acid, chloroacetic acid, dichloroacetic acid, trichloroacetic acid, succinic acid, and malic acid, and amino acids, such as glycine, aspartic acid, glutamic acid, histidine, lysine, and arginine, are inactive as cofactors
the carboxylic acids including hydroxyacetic acid, cyanoacetic acid, bromoacetic acid, chloroacetic acid, dichloroacetic acid, trichloroacetic acid, succinic acid, and malic acid, and amino acids, such as glycine, aspartic acid, glutamic acid, histidine, lysine, and arginine, are inactive as cofactors
the oxidative brominating activity of an organic solvent-tolerant recombinant metal-free bromoperoxidase C-terminally tagged BPO-A1 (rBPO-A1) from Streptomyces aureofaciens depends on various additives. These include carboxylic acids, used as cofactors, and alcohols, used as water-miscible organic solvents. Propanoic acid, 2-methylpropanoic acid, and 1-butanoic acid enhanced rBPO-A1's activity by 13.7, 8.0, and 4.6fold, respectively, compared to that obtained with acetic acid. The decrease in the activity associated with changes from primary to tertiary fatty chains can be attributed to increased steric hindrance. Carboxylic acid binding structure analysis, overview
the oxidative brominating activity of an organic solvent-tolerant recombinant metal-free bromoperoxidase C-terminally tagged BPO-A1 (rBPO-A1) from Streptomyces aureofaciens depends on various additives. These include carboxylic acids, used as cofactors, and alcohols, used as water-miscible organic solvents. Propanoic acid, 2-methylpropanoic acid, and 1-butanoic acid enhanced rBPO-A1's activity by 13.7, 8.0, and 4.6fold, respectively, compared to that obtained with acetic acid. The decrease in the activity associated with changes from primary to tertiary fatty chains can be attributed to increased steric hindrance. Carboxylic acid binding structure analysis, overview