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1.11.1.18: bromide peroxidase

This is an abbreviated version!
For detailed information about bromide peroxidase, go to the full flat file.

Word Map on EC 1.11.1.18

Reaction

RH
+
HBr
+
H2O2
=
RBr
+ 2 H2O

Synonyms

apobromoperoxidase I, BPO, BPO 1, BPO 2, BPO I, BPO-A1, BPO-A2, BPO1, bpoA1, bromooperoxidase, bromoperoxidase, bromoperoxidase A2, bromoperoxidase I, bromoperoxidase II, bromoperoxidase-catalase, BrPO, CoVBPO, CVBPO, EC 1.11.1.10, haeme-thiolate peroxidase, HAP phytase, metal-free bromoperoxidase, More, non-haem bromoperoxidase, non-haem bromoperoxidase BPO 1, non-haem bromoperoxidase BPO 2, non-haem bromoperoxidase BPO-A2, non-heme haloperoxidases, non-heme-type bromoperoxidase, nonhaem-type bromoperoxidase BPO 1a, nonhaem-type bromoperoxidase BPO 1b, nonhaem-type bromoperoxidase BPO 3, nonheme bromoperoxidase, nVBPO, perhydrolase, rVBPO, St-Phy, sVBPO, V-BPO, V-BrPO, V-containing-haloperoxidase, vanadate haloperoxidase, vanadate-dependent bromoperoxidase I, vanadium bromoperoxidase, vanadium containing bromoperoxidase, vanadium-bromoperoxidase, vanadium-containing bromoperoxidase, vanadium-dependent bromoperoxidase, vanadium-dependent bromoperoxidase 2, vanadium-dependent haloperoxidase, vanadium-dependent peroxidase, vBPO, VBPO1, VBPO2, VBrPO, VBrPO(AnI), VBrPO(AnII)

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.18 bromide peroxidase

Temperature Stability

Temperature Stability on EC 1.11.1.18 - bromide peroxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 80
reaction activity in the presence of recombinant BPO-A1 peaks at 60°C whereas peak in the non-enzymatic activity of H2O2 is not observed in temperature range of 10-70°C. The increase in the activity of the enzyme with propanoic acid around 10-50°C is due to the peroxidation step because high activity in the nonenzymatic oxidative bromination step is maintained at low temperature, which suppresses the decomposition of the active species generated by the reaction between peracid and Br-. The active species is heat-labile. The significant decrease in activity around 65-70°C is attributed to decomposition of the active species. The native BPO-A1 possesses high stability up to 80°C. Recombinant BPO-A1 possesses high peroxidating activity at high temperatures
50
-
activity remains constant for at least 4 h
57
-
20 min, 50% loss of activity, enzyme preincubated without metal ion
65
-
20 min, recombinantly expressed enzyme is stable, native enzyme is completely inactivated
72
-
Tm-value
75
-
20 min, 50% loss of activity, enzyme preincubated with 1 mM vanadate
87
-
20 min, 50% loss of activity, enzyme preincubated with 1 mM Ca2+
92
-
20 min, 50% loss of activity, enzyme preincubated with 1 mM Ca2+ and 1 mM vanadium
additional information