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1.11.1.18: bromide peroxidase

This is an abbreviated version!
For detailed information about bromide peroxidase, go to the full flat file.

Word Map on EC 1.11.1.18

Reaction

RH
+
HBr
+
H2O2
=
RBr
+ 2 H2O

Synonyms

apobromoperoxidase I, BPO, BPO 1, BPO 2, BPO I, BPO-A1, BPO-A2, BPO1, bpoA1, bromooperoxidase, bromoperoxidase, bromoperoxidase A2, bromoperoxidase I, bromoperoxidase II, bromoperoxidase-catalase, BrPO, CoVBPO, CVBPO, EC 1.11.1.10, haeme-thiolate peroxidase, HAP phytase, metal-free bromoperoxidase, More, non-haem bromoperoxidase, non-haem bromoperoxidase BPO 1, non-haem bromoperoxidase BPO 2, non-haem bromoperoxidase BPO-A2, non-heme haloperoxidases, non-heme-type bromoperoxidase, nonhaem-type bromoperoxidase BPO 1a, nonhaem-type bromoperoxidase BPO 1b, nonhaem-type bromoperoxidase BPO 3, nonheme bromoperoxidase, nVBPO, perhydrolase, rVBPO, St-Phy, sVBPO, V-BPO, V-BrPO, V-containing-haloperoxidase, vanadate haloperoxidase, vanadate-dependent bromoperoxidase I, vanadium bromoperoxidase, vanadium containing bromoperoxidase, vanadium-bromoperoxidase, vanadium-containing bromoperoxidase, vanadium-dependent bromoperoxidase, vanadium-dependent bromoperoxidase 2, vanadium-dependent haloperoxidase, vanadium-dependent peroxidase, vBPO, VBPO1, VBPO2, VBrPO, VBrPO(AnI), VBrPO(AnII)

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.18 bromide peroxidase

Cloned

Cloned on EC 1.11.1.18 - bromide peroxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme is overproduced in Streptomyces lividans TK64, up to 30000 times compared to Streptomyces aureofaciens
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli. Recombinant enzyme and its mutants have no enzyme activities, unless they are activated by Na3VO4
expression in Saccharomyces cerevisiae
-
gene bpo-A1, recombiant expression of His-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3)
gene phyA, DNA and amino acid sequence determination and analysis, recombinant expression of the codon-optimized phytase gene in Pichia pastoris strain X-33, quantitative real-time PCR for gene copy number determination
overexpressed in Escherichia coli. The enzyme is found to be predominantly in the form of inclusion bodies
-
the gene is cloned in the positive selection vector pIJ699 and expression in Streptomyces lividans TK64. The cloned bromoperoxidase is overproduced up to 2800fold by the Streptomyces lividans transformant
-