1.11.1.18: bromide peroxidase

This is an abbreviated version!
For detailed information about bromide peroxidase, go to the full flat file.

Word Map on EC 1.11.1.18

Reaction

RH
+
HBr
+
H2O2
=
RBr
+ 2 H2O

Synonyms

apobromoperoxidase I, BPO, BPO 1, BPO 2, BPO I, BPO-A1, BPO-A2, BPO1, bromoperoxidase, bromoperoxidase A2, bromoperoxidase I, bromoperoxidase-catalase, BrPO, CoVBPO, CVBPO, EC 1.11.1.10, non-haem bromoperoxidase, non-haem bromoperoxidase BPO 1, non-haem bromoperoxidase BPO 2, non-haem bromoperoxidase BPO-A2, non-heme haloperoxidases, non-heme-type bromoperoxidase, nonhaem-type bromoperoxidase BPO 1a, nonhaem-type bromoperoxidase BPO 1b, nonhaem-type bromoperoxidase BPO 3, nonheme bromoperoxidase, nVBPO, rVBPO, sVBPO, V-BPO, V-BrPO, vanadate-dependent bromoperoxidase I, vanadium bromoperoxidase, vanadium containing bromoperoxidase, vanadium-containing bromoperoxidase, vanadium-dependent bromoperoxidase, vanadium-dependent bromoperoxidase 2, vanadium-dependent haloperoxidase, vanadium-dependent peroxidase, vBPO, VBPO1, VBPO2, VBrPO(AnI)

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.18 bromide peroxidase

Cloned

Cloned on EC 1.11.1.18 - bromide peroxidase

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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
enzyme is overproduced in Streptomyces lividans TK64, up to 30000 times compared to Streptomyces aureofaciens
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
-
expression in Escherichia coli. Recombinant enzyme and its mutants have no enzyme activities, unless they are activated by Na3VO4
expression in Saccharomyces cerevisiae
-
overexpressed in Escherichia coli. The enzyme is found to be predominantly in the form of inclusion bodies
-
the gene is cloned in the positive selection vector pIJ699 and expression in Streptomyces lividans TK64. The cloned bromoperoxidase is overproduced up to 2800fold by the Streptomyces lividans transformant
-