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(2E,6E)-8-(N-methyl-2-aminobenzoyloxy)-3,7-dimethyl-2,6-octandien-1-diphosphate + 8 isopentenyl diphosphate
?
-
a fluorescent analog of farnesyl diphosphate
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans,poly-cis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
diphosphate + ditrans,polycis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl thiodiphosphate + isopentenyl diphosphate
?
-
weak activity, 10000000fold lower than with (2E,6E)-farnesyl diphosphate
-
-
?
(2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
(2Z,6E)-famesyl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
(2Z,6E,10E,14E)-farnesylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
(2Z,6Z,10E,14E)-farnesylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
(E)-3-methyl-3-pentenyl diphosphate + isopentenyl diphosphate
?
-
reactive as substrate and competitive inhibition profile
-
-
?
(Z,E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
(Z,E,E,E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
2-amideanilinogeranyl diphosphate + isopentenyl diphosphate
?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate
9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
7-(2,6-dimethyl-8-diphospho-2,6-octadienyloxy)-8-methyl-4-trifluoromethyl-chromen-2-one geranyl diphosphate + isopentenyl diphosphate
?
-
fluorescent analogue of (2E,6E)-farnesyl diphosphate, alternative substrate and inhibitor
-
-
?
di-trans-poly-cis-decaprenyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + undecaprenyl diphosphate
-
neither dimethylallyl diphosphate nor geranyl diphosphate act as priming substrate for the enzyme
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
farnesyl phosphate + isopentenyl diphosphate
phosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
low activity
-
-
?
geranyl diphosphate + isopentenyl diphosphate
?
-
C10 GPP displays a 90fold larger Km value compared with (2E,6E)-farnesyl diphosphate
-
-
?
geranyl diphosphate + isopentenyl diphosphate
diphosphate + ?
-
less effective than farnesyl diphosphate
-
-
?
geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
containing a larger C20 hydrocarbon tail, is an equally good substrate
-
-
?
geranylgeranyl diphosphate + isopentenyl diphosphate
diphosphate + ?
-
as effective as farnesyl diphosphate
-
-
?
isopentenyl diphosphate + (E,E)-(2-diazo-3-trifluoropropionyloxy)geranyl diphosphate
(E,E)-(2-diazo-3-trifluoropropionyloxy)polyprenyl diphosphate + diphosphate
isopentenyl diphosphate + DELTA3-isopentenyl diphosphate
?
-
-
-
-
?
isopentenyl diphosphate + dimethylallyl diphosphate
?
-
2% of the activity with farnesyl diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
isopentenyl diphosphate + geranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
isopentenyl diphosphate + geranylneryl diphosphate
?
-
cis,trans,trans-geranylneryl diphosphate
-
-
?
isopentenyl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
isopentenyl diphosphate + neryl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
isopentenyl diphosphate + S-farnesyl thiodiphosphate
?
-
weak activity, 10000000fold lower than with farnesyl diphosphate
-
-
?
N-(4-benzoylphenyl)-4-hydroxy-2-oxo-5-phenethyl-2,5-dihydro-1H-pyrrole-3-carboxamide + isopentenyl diphosphate
?
-
Photo-probe
-
-
?
neryl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
additional information
?
-
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
the enzyme catalyzes the synthesis of the C55 lipid undecaprenyl diphosphate through eight consecutive condensations of isopentenyl pyrophosphate
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
the enzyme catalyzes the synthesis of the C55 lipid undecaprenyl diphosphate through eight consecutive condensations of isopentenyl pyrophosphate
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
637485, 637488, 637494, 637495, 657857, 659232, 660464, 707467, 708864, 721275, 721794, 758567 -
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
quantification of product distribution of UPPs catalyzed coupling of (2E,6E)-farnesyl diphosphate and isopentenyl diphosphate under various reaction conditions
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
catalyzes the condensation of eight molecules of isopentenyl pyrophosphate (IPP) with farnesyl diphosphate (FPP) to generate C55 undecaprenyl diphosphate. With a variety of different ratios of enzyme to substrate and FPP to IPP in the presence or absence of Triton, different product distributions are found
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
UPPS catalyzes eight consecutive condensation steps of IPP with FPP to form C55 final product
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
the enzyme is responsible for the biogenesis of undecaprenyl phosphate, an indispensable glycosyl carrier lipid in bacterial cell wall biosynthesis
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
the enzyme catalyzes the synthesis of the C55 lipid undecaprenyl diphosphate through eight consecutive condensations of isopentenyl pyrophosphate
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
the enzyme is responsible for the biogenesis of undecaprenyl phosphate, an indispensable glycosyl carrier lipid in bacterial cell wall biosynthesis
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
the enzyme catalyzes the synthesis of the C55 lipid undecaprenyl diphosphate through eight consecutive condensations of isopentenyl pyrophosphate
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
trans,trans-farnesyl diphosphate
C55 polyprenyl diphosphate with isoprene residues with cis stereochemistry
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
catalyzes the cis-prenyl chain elongation onto trans,trans-farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP). The product undecaprenyl diphosphate is indispensable for the biosynthesis of bacterial cell walls
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
catalyzes the cis-prenyl chain elongation onto trans,trans-farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP). The product undecaprenyl diphosphate is indispensable for the biosynthesis of bacterial cell walls
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
the enzyme catalyzes the synthesis of the C55 lipid undecaprenyl diphosphate through eight consecutive condensations of isopentenyl pyrophosphate
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
the enzyme catalyzes the synthesis of the C55 lipid undecaprenyl diphosphate through eight consecutive condensations of isopentenyl pyrophosphate
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
(2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
?
(2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
?
(Z,E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
(Z,E)-geranylgeranyl diphosphate + isopentenyl diphosphate
?
-
-
-
-
?
2-amideanilinogeranyl diphosphate + isopentenyl diphosphate
?
lower activity, the fluorescent substrate analogue undergoes a 1.2fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays
ESI-MS analysis suggests that these major product peaks correspond to six to nine Z-configuration isoprenes incorporated by the UPPS protein
-
?
2-amideanilinogeranyl diphosphate + isopentenyl diphosphate
?
lower activity, the fluorescent substrate analogue undergoes a 1.2fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays
ESI-MS analysis suggests that these major product peaks correspond to six to nine Z-configuration isoprenes incorporated by the UPPS protein
-
?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate
9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays
-
-
?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate
9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays
-
-
?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate
9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays
-
-
?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate
9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays
-
-
?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate
9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays
-
-
?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate
9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays
-
-
?
di-trans-poly-cis-decaprenyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
di-trans-poly-cis-decaprenyl diphosphate is farnesyl diphosphate
-
-
?
di-trans-poly-cis-decaprenyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
di-trans-poly-cis-decaprenyl diphosphate is farnesyl diphosphate, the diphosphate head group of farnesyl diphosphate is bound to the backbone of NHs pf G29 and R30 as well as the side chains of N28, R30, and R39 through hydrogen bonds, H43 may stabilize the farnesyl cation intermediate during catalysis being near to the C2 carbon of farnesyl diphosphate
-
-
?
di-trans-poly-cis-decaprenyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
di-trans-poly-cis-decaprenyl diphosphate is farnesyl diphosphate, isopentenyl diphosphate binding mode determination
-
-
?
di-trans-poly-cis-decaprenyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
di-trans-poly-cis-decaprenyl diphosphate is farnesyl diphosphate, isopentenyl diphosphate binding mode determination
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
consecutive condensation of 8 molecules of isopentenyl diphosphate with farnesyl diphosphate to form the lipid carrier undecaprenyl diphosphate to mediate bacterial peptidoglycan synthesis
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
consecutive condensation of 8 molecules of isopentenyl diphosphate with farnesyl diphosphate to form the lipid carrier undecaprenyl diphosphate to mediate bacterial peptidoglycan synthesis
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
consecutive condensation of 8 molecules of isopentenyl diphosphate with farnesyl diphosphate to form the lipid carrier undecaprenyl diphosphate to mediate bacterial peptidoglycan synthesis, the final product has C55 chain length
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
consecutive condensation of 8 molecules of isopentenyl diphosphate with farnesyl diphosphate to form the lipid carrier undecaprenyl diphosphate to mediate bacterial peptidoglycan synthesis, the final product has C55-C60 chain length, molecular 3D-structural modeling of the reaction product
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
stereochemistry of the reaction
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
the phosphate head group of farnesyl diphosphate is bound to the positively charged Arg residues and the hydrocarbon moiety interacts with hydrophobic amino acids including L85, L88, and F89, located on the alpha3-helix of the enzyme
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
sequential condensation of 8 molecules of isopentenyl diphosphate in the cis-configuration into farnesyl diphosphate to produce undecaprenyl diphosphate, indispensable for the biosynthesis of bacterial cell wall
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
sequential condensation of 8 molecules of isopentenyl diphosphate in the cis-configuration into farnesyl diphosphate to produce undecaprenyl diphosphate, indispensable for the biosynthesis of bacterial cell wall
-
-
?
isopentenyl diphosphate + (E,E)-(2-diazo-3-trifluoropropionyloxy)geranyl diphosphate
(E,E)-(2-diazo-3-trifluoropropionyloxy)polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + (E,E)-(2-diazo-3-trifluoropropionyloxy)geranyl diphosphate
(E,E)-(2-diazo-3-trifluoropropionyloxy)polyprenyl diphosphate + diphosphate
-
47% of the activity with trans,trans-farnesyl diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
(E,E)-farnesyl diphosphate or (Z,E)-farnesyl diphosphate
C50-polyprenyl diphosphate and C55-polyprenyl diphosphate in the ratio of 3:1 from (Z,E,E,E)-farnesylgeranyl diphosphate and in the ratio 3:2 from (Z,Z,E,E)-farnesylgeranyl diphosphate, C50-polyprenyl diphosphate and C55-polyprenyl diphosphate with Z,E mixed stereochemistry, formation of C50-polyprenyl diphosphate and C55-polyprenyl diphosphate in the ratio of 1:4 from (E,E)-farnesyl diphosphate and in the ratio of 4:1 from (Z,E)-farnesyl diphosphate
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
(Z,E,E,E)-farnesylgeranyl diphosphate or (Z,Z,E,E)-farnesylgeranyl diphosphate
C50-polyprenyl diphosphate and C55-polyprenyl diphosphate in the ratio of 3:1 from (Z,E,E,E)-farnesylgeranyl diphosphate and in the ratio 3:2 from (Z,Z,E,E)-farnesylgeranyl diphosphate, C50-polyprenyl diphosphate and C55-polyprenyl diphosphate with Z,E mixed stereochemistry, formation of C50-polyprenyl diphosphate and C55-polyprenyl diphosphate in the ratio of 1:4 from (E,E)-farnesyl diphosphate and in the ratio of 4:1 from (Z,E)-farnesyl diphosphate
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
the major product of the wild-type enzyme, and mutant enzymes H103A, V103A, V105A and I62A in presence of Triton is C55-polyprenyl diphosphate. Mutant enzyme L137A produces C55-polyprenyl diphosphate, C60-polyprenyl diphosphate and C65-polyprenyl diphosphate in the ration 55:41:4
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
product distribution under various reaction conditions. In the presence of excess farnesyl diphosphate the intermediates C25-C50 accumulate
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
with 0.02% Triton X-100 the main product is the C55-compound, C50-compound is the minor product. With 0.1% Triton X-100 the C50-polyprenyl diphosphate is the main product, the C55-polyprenyl diphosphate and the C45-polyprenyl diphosphate are minor products. With 0.5% Triton X-100 the C50-polyprenyl diphosphate compound and the C45-compound are the main products, the C55-compound is not formed
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
trans,trans-farnesyl diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
major products are undecaprenol and probably a longer chain polyprenol
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
cis,trans-farnesyl diphosphate
C55-compound is formed as major product from trans,trans-farnesyl diphosphate, the C50-compound is formed as the major product from cis,trans-farnesyl diphosphate
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
farnesyl diphosphate with mixed stereochemistry or trans,trans stereochemistry
C55-polyprenyl diphosphate is the major product
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
trans,trans-farnesyl diphosphate and cis,trans-farnesyl diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
trans,trans-farnesyl diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
trans,trans-farnesyl diphosphate
C55 polyprenyl diphosphate with isoprene residues with cis stereochemistry
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
trans,trans-farnesyl diphosphate
C55-compound is formed as major product from trans,trans-farnesyl diphosphate, the C50-compound is formed as the major product from cis,trans-farnesyl diphosphate
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
cis,trans-farnesyl diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
(E,E)-farnesyl diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
(Z,E,E)-farnesyl diphosphate
E193Q, R197S, E201Q, R203S, D221A, and D226A show product distribution pattern similar to that of the wild-type enzyme, which produces C55 prenyl diphosphate as its major product as well as minor amounts of some intermediates having shorter prenyl chains and C60 product. The major products of the mutant enzymes S74A and E216Q contain C35 and C50 prenyl chain length. Mutant enzyme F73A produces a mixture of C30 and C35 products
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
trans,trans-farnesyl diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
trans,trans-farnesyl diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
trans,trans-farnesyl diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
(E,E)-farnesyl diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
(Z,E,E)-farnesyl diphosphate
E193Q, R197S, E201Q, R203S, D221A, and D226A show product distribution pattern similar to that of the wild-type enzyme, which produces C55 prenyl diphosphate as its major product as well as minor amounts of some intermediates having shorter prenyl chains and C60 product. The major products of the mutant enzymes S74A and E216Q contain C35 and C50 prenyl chain length. Mutant enzyme F73A produces a mixture of C30 and C35 products
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
the C55 polymer is the major product in presence of Triton X-100, without Triton C55-C75 polyprenyl diphosphates are generated
?
isopentenyl diphosphate + farnesyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
Salmonella newington
-
-
-
-
?
isopentenyl diphosphate + geranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + geranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + geranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
the C50-compound is the major product
?
isopentenyl diphosphate + geranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
about 20% of the activity with farnesyl diphosphate
C55-polyprenyl diphosphate is the major product
?
isopentenyl diphosphate + geranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
(E,E,E)-geranylgeranyl diphosphate or (Z,E,E)-geranylgeranyl diphosphate
C50-prenyl diphosphate and C55-prenyl diphosphate in the ratio of 2:3 from (E,E,E)-geranyl diphosphate and in the ratio 1:3 from (Z,E,E)-geranylgeranyl diphosphate
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
trans,trans,trans-geranylgeranyl diphosphate and cis,trans,trans-geranylgeranyl diphosphate
the C55-compound is formed as major product
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
trans,trans,trans-geranylgeranyl diphosphate
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
(Z,E,E)-geranylgeranyl diphosphate
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
trans,trans,trans-geranylgeranyl diphosphate and cis,trans,trans-geranylgeranyl diphosphate
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
(Z,E,E)-geranylgeranyl diphosphate
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
(Z,E,E)-geranylgeranyl diphosphate
-
-
?
isopentenyl diphosphate + neryl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + neryl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
C45 prenyl diphosphate, C50 prenyl diphosphate and C55 prenyl diphosphate in the ratio of 2:10:1
?
isopentenyl diphosphate + neryl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
isopentenyl diphosphate + neryl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
the C50-compound is the major product
?
isopentenyl diphosphate + neryl diphosphate
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
-
?
additional information
?
-
-
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
-
-
?
additional information
?
-
-
kinetic experiments with artificial substrate homologues
-
-
?
additional information
?
-
-
Z-isomer ZHIPP, (Z)-3-methyl-3-pentenyl diphosphate, which is not accepted as substrate by the enzyme, exhibits no inhibition on the synthase reaction
-
-
?
additional information
?
-
development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity
-
-
?
additional information
?
-
-
development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity
-
-
?
additional information
?
-
development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity
-
-
?
additional information
?
-
-
role of the hydrocarbon moiety of the allylic substrate in the reaction
-
-
?
additional information
?
-
-
catalyzes the consecutive condensation reactions of a farnesyl diphosphate (FPP) with eight isopentenyl diphosphates (IPP), in which new cis-double bonds are formed, to generate undecaprenyl diphosphate that serves as a lipid carrier for peptidoglycan synthesis of bacterial cell wall
-
-
?
additional information
?
-
catalyzes the consecutive condensation reactions of a farnesyl diphosphate (FPP) with eight isopentenyl diphosphates (IPP), in which new cis-double bonds are formed, to generate undecaprenyl diphosphate that serves as a lipid carrier for peptidoglycan synthesis of bacterial cell wall
-
-
?
additional information
?
-
development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate
-
-
?
additional information
?
-
-
development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate
-
-
?
additional information
?
-
development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate
-
-
?
additional information
?
-
-
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
-
-
?
additional information
?
-
-
reactivities of the allylic substrates increase with chain length: C10, C15, C20. Each new isoprene unit added has a cis-configuration
-
-
?
additional information
?
-
-
the product undecaprenyl diphosphate is indispensable for the biosynthesis of bacterial cell walls
-
-
?
additional information
?
-
the product undecaprenyl diphosphate is indispensable for the biosynthesis of bacterial cell walls
-
-
?
additional information
?
-
-
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
-
-
?
additional information
?
-
-
artificial substrate analogs, 3-desmethyl farnesyl diphosphate and 3-desmethyl Z-geranylgeranyl diphosphate are not used as substrates. The methyl group at the 3-position of the allylic substrate is important in the undecaprenyl diphosphate synthase reaction. The binding sites for the natural substrate farnesyl diphosphate and those for the intermediate allylic diphosphate, which contains the cis-prenyl unit, are different during the cis-prenyl chain elongation reaction
-
-
?
additional information
?
-
-
catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate
-
-
?
additional information
?
-
catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate
-
-
?
additional information
?
-
-
artificial substrate analogs, 3-desmethyl farnesyl diphosphate and 3-desmethyl (2Z,6E,10E)-geranylgeranyl diphosphate are not used as substrates. The methyl group at the 3-position of the allylic substrate is important in the undecaprenyl diphosphate synthase reaction. The binding sites for the natural substrate farnesyl diphosphate and those for the intermediate allylic diphosphate, which contains the cis-prenyl unit, are different during the cis-prenyl chain elongation reaction
-
-
?
additional information
?
-
-
artificial substrate analogs, 3-desmethyl farnesyl diphosphate and 3-desmethyl Z-geranylgeranyl diphosphate are not used as substrates. The methyl group at the 3-position of the allylic substrate is important in the undecaprenyl diphosphate synthase reaction. The binding sites for the natural substrate farnesyl diphosphate and those for the intermediate allylic diphosphate, which contains the cis-prenyl unit, are different during the cis-prenyl chain elongation reaction
-
-
?
additional information
?
-
-
artificial substrate analogs, 3-desmethyl farnesyl diphosphate and 3-desmethyl (2Z,6E,10E)-geranylgeranyl diphosphate are not used as substrates. The methyl group at the 3-position of the allylic substrate is important in the undecaprenyl diphosphate synthase reaction. The binding sites for the natural substrate farnesyl diphosphate and those for the intermediate allylic diphosphate, which contains the cis-prenyl unit, are different during the cis-prenyl chain elongation reaction
-
-
?
additional information
?
-
-
the product undecaprenyl diphosphate is indispensable for the biosynthesis of bacterial cell walls
-
-
?
additional information
?
-
the product undecaprenyl diphosphate is indispensable for the biosynthesis of bacterial cell walls
-
-
?
additional information
?
-
-
catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate
-
-
?
additional information
?
-
catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate
-
-
?
additional information
?
-
-
the synthesized long-chain dehydrodolichyl diphosphate serves as a precursor of glycosyl carrier in glycoprotein biosynthesis in eukaryotes
-
-
?
additional information
?
-
the precursor for C55-P, undecaprenyl diphosphate (UPP), is synthesized by the addition of eight C5 isopentenyl units (cis[Z]-configuration) onto C15-PP (all-trans [E])-farnesyl diphosphate (FPP) catalyzed by UPP synthase
-
-
?
additional information
?
-
-
the precursor for C55-P, undecaprenyl diphosphate (UPP), is synthesized by the addition of eight C5 isopentenyl units (cis[Z]-configuration) onto C15-PP (all-trans [E])-farnesyl diphosphate (FPP) catalyzed by UPP synthase
-
-
?
additional information
?
-
the precursor for C55-P, undecaprenyl diphosphate (UPP), is synthesized by the addition of eight C5 isopentenyl units (cis[Z]-configuration) onto C15-PP (all-trans [E])-farnesyl diphosphate (FPP) catalyzed by UPP synthase
-
-
?
additional information
?
-
development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate
-
-
?
additional information
?
-
-
development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate
-
-
?
additional information
?
-
development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
additional information
?
-
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
catalyzes the condensation of eight molecules of isopentenyl pyrophosphate (IPP) with farnesyl diphosphate (FPP) to generate C55 undecaprenyl diphosphate. With a variety of different ratios of enzyme to substrate and FPP to IPP in the presence or absence of Triton, different product distributions are found
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
UPPS catalyzes eight consecutive condensation steps of IPP with FPP to form C55 final product
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
the enzyme is responsible for the biogenesis of undecaprenyl phosphate, an indispensable glycosyl carrier lipid in bacterial cell wall biosynthesis
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
the enzyme is responsible for the biogenesis of undecaprenyl phosphate, an indispensable glycosyl carrier lipid in bacterial cell wall biosynthesis
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
catalyzes the cis-prenyl chain elongation onto trans,trans-farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP). The product undecaprenyl diphosphate is indispensable for the biosynthesis of bacterial cell walls
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
catalyzes the cis-prenyl chain elongation onto trans,trans-farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP). The product undecaprenyl diphosphate is indispensable for the biosynthesis of bacterial cell walls
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
consecutive condensation of 8 molecules of isopentenyl diphosphate with farnesyl diphosphate to form the lipid carrier undecaprenyl diphosphate to mediate bacterial peptidoglycan synthesis
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
consecutive condensation of 8 molecules of isopentenyl diphosphate with farnesyl diphosphate to form the lipid carrier undecaprenyl diphosphate to mediate bacterial peptidoglycan synthesis
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
consecutive condensation of 8 molecules of isopentenyl diphosphate with farnesyl diphosphate to form the lipid carrier undecaprenyl diphosphate to mediate bacterial peptidoglycan synthesis, the final product has C55 chain length
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
consecutive condensation of 8 molecules of isopentenyl diphosphate with farnesyl diphosphate to form the lipid carrier undecaprenyl diphosphate to mediate bacterial peptidoglycan synthesis, the final product has C55-C60 chain length, molecular 3D-structural modeling of the reaction product
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
sequential condensation of 8 molecules of isopentenyl diphosphate in the cis-configuration into farnesyl diphosphate to produce undecaprenyl diphosphate, indispensable for the biosynthesis of bacterial cell wall
-
-
?
farnesyl diphosphate + isopentenyl diphosphate
diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
sequential condensation of 8 molecules of isopentenyl diphosphate in the cis-configuration into farnesyl diphosphate to produce undecaprenyl diphosphate, indispensable for the biosynthesis of bacterial cell wall
-
-
?
additional information
?
-
-
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
-
-
?
additional information
?
-
-
kinetic experiments with artificial substrate homologues
-
-
?
additional information
?
-
-
catalyzes the consecutive condensation reactions of a farnesyl diphosphate (FPP) with eight isopentenyl diphosphates (IPP), in which new cis-double bonds are formed, to generate undecaprenyl diphosphate that serves as a lipid carrier for peptidoglycan synthesis of bacterial cell wall
-
-
?
additional information
?
-
catalyzes the consecutive condensation reactions of a farnesyl diphosphate (FPP) with eight isopentenyl diphosphates (IPP), in which new cis-double bonds are formed, to generate undecaprenyl diphosphate that serves as a lipid carrier for peptidoglycan synthesis of bacterial cell wall
-
-
?
additional information
?
-
-
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
-
-
?
additional information
?
-
-
the product undecaprenyl diphosphate is indispensable for the biosynthesis of bacterial cell walls
-
-
?
additional information
?
-
the product undecaprenyl diphosphate is indispensable for the biosynthesis of bacterial cell walls
-
-
?
additional information
?
-
-
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
-
-
?
additional information
?
-
-
catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate
-
-
?
additional information
?
-
catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate
-
-
?
additional information
?
-
-
the product undecaprenyl diphosphate is indispensable for the biosynthesis of bacterial cell walls
-
-
?
additional information
?
-
the product undecaprenyl diphosphate is indispensable for the biosynthesis of bacterial cell walls
-
-
?
additional information
?
-
-
catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate
-
-
?
additional information
?
-
catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate
-
-
?
additional information
?
-
-
the synthesized long-chain dehydrodolichyl diphosphate serves as a precursor of glycosyl carrier in glycoprotein biosynthesis in eukaryotes
-
-
?
additional information
?
-
the precursor for C55-P, undecaprenyl diphosphate (UPP), is synthesized by the addition of eight C5 isopentenyl units (cis[Z]-configuration) onto C15-PP (all-trans [E])-farnesyl diphosphate (FPP) catalyzed by UPP synthase
-
-
?
additional information
?
-
-
the precursor for C55-P, undecaprenyl diphosphate (UPP), is synthesized by the addition of eight C5 isopentenyl units (cis[Z]-configuration) onto C15-PP (all-trans [E])-farnesyl diphosphate (FPP) catalyzed by UPP synthase
-
-
?
additional information
?
-
the precursor for C55-P, undecaprenyl diphosphate (UPP), is synthesized by the addition of eight C5 isopentenyl units (cis[Z]-configuration) onto C15-PP (all-trans [E])-farnesyl diphosphate (FPP) catalyzed by UPP synthase
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(1-hydroxy-2-phenylethane-1,1-diyl)bis(phosphonic acid)
-
(1-hydroxy-2-[3'-[(naphthalene-2-sulfonyl)amino][1,1'-biphenyl]-3-yl]ethane-1,1-diyl)bis(phosphonic acid)
-
(2E,6E)-farnesyl thiodiphosphate
(2E,6E,10E)-4-methylfarnesyl diphosphate
-
competitive inhibitor against (2Z,6E,10E)-geranylgeranyl diphosphate
(2S,3R,4S,5S,6R)-2-(3-((4-((3-(4,5-dihydro-1H-imidazol-2-yl)-phenyl)ethynyl)phenyl)ethynyl)phenoxy)-6-(hydroxymethyl)-tetrahydro-2H-pyran-3,4,5-triol
(2Z)-2-hydroxy-4-oxo-4-[[3-(3-phenoxyphenyl)propyl]amino]but-2-enoic acid
-
(2Z)-2-hydroxy-4-[3-[3-(octyloxy)phenoxy]phenyl]-4-oxobut-2-enoic acid
-
(2Z)-2-hydroxy-4-[4-(octyloxy)phenyl]-4-oxobut-2-enoic acid
-
(2Z)-4-([3-[3-(hexyloxy)phenyl]propyl]amino)-2-hydroxy-4-oxobut-2-enoic acid
-
(2Z)-4-[3-[3-(decyloxy)phenoxy]phenyl]-2-hydroxy-4-oxobut-2-enoic acid
-
(2Z)-4-[[3-([1,1'-biphenyl]-3-yl)propyl]amino]-2-hydroxy-4-oxobut-2-enoic acid
-
(2Z,6E,10E)-4-methyl-geranylgeranyl diphosphate
-
allylic substrate homologue, competitive inhibitor against the allylic primer. It also acts as a strong noncompetitive inhibitor against isopentenyl diphosphate
(4aS,8aS)-4-hydroxy-2-oxo-1,2,4a,5,6,7,8,8a-octahydro[1,6]naphthyridine-3-carboxylic acid (4-piperidin-1-ylphenyl)amide
-
-
(5-bromo-2-[[3-(octyloxy)phenyl]methoxy]phenyl)phosphonic acid
-
-
(E)-3-methyl-3-pentenyl diphosphate
-
reactive as substrate and competitive inhibition profile
(E,E)-[1-3H]-(2-diazo-3-trifluoropropionyloxy)geranyl diphosphate
-
-
(S)-farnesyl thiodiphosphate
-
an extremely poor substrate for UPPs
1,4-bis(6-(1,4,5,6-tetrahydropyrimidin-2-yl)-1H-indol-2-yl)-benzene
1,4-bis(6-(4,5-dihydro-1H-imidazol-2-yl)-1H-indol-2-yl)benzene
1-(2-hydroxy-2,2-diphosphonoethyl)-3-phenylpyridin-1-ium
-
1-[3-fluoro-4-(4-[[2-(thiophen-2-yl)-1,3-thiazol-4-yl]methyl]piperazin-1-yl)phenyl]ethan-1-one
-
1-[[3-(hexyloxy)phenyl]methyl]-4-hydroxy-2-oxo-1,2-dihydropyridine-3-carboxylic acid
-
2,2'-(5,5'-(1,3-phenylenebis(ethyne-2,1-diyl))bis(3-bromo-5,1-phenylene))diethanamine
2,2'-(E)-ethene-1,2-diylbis[6-(4,5-dihydro-1H-imidazol-2-yl)-1H-indole]
2,6-bis(5-bromo-2-hydroxyphenyl)-3,5-dimethylpiperidin-4-one
-
2-(2-[[5-(1-benzofuran-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl]sulfanyl]acetamido)benzoic acid
-
2-hydroxy-6-(undecyloxy)benzoic acid
-
2-methoxy-6-[3-(octyloxy)benzamido]benzoic acid
-
-
2-[3-(decyloxy)benzamido]-5-nitrobenzoic acid
-
-
2-[3-(hexyloxy)benzamido]benzoic acid
-
-
2-[3-(octyloxy)benzamido]-5-(trifluoromethoxy)benzoic acid
-
-
2-[3-(octyloxy)benzamido]-5-phosphonobenzoic acid
-
-
2-[3-(octyloxy)benzamido]benzoic acid
-
-
2-[4-[(E)-2-chloro-1,2-diphenylethenyl]phenoxy]-N,N-diethylethan-1-amine
-
2-[methyl[3-(octyloxy)benzoyl]amino]benzoic acid
-
-
2-[[(2E)-2-cyano-3-[1-[(naphthalen-1-yl)methyl]-1H-indol-3-yl]prop-2-enoyl]amino]benzoic acid
-
2-[[(2E)-2-cyano-3-[3-[(4-fluorophenyl)methoxy]phenyl]prop-2-enoyl]amino]benzoic acid
-
2-[[3-(3,4-dimethylphenoxy)phenyl]carbamoyl]benzoic acid
-
3-(2-chlorophenyl)-5-methyl-N-[4-(propan-2-yl)phenyl]-1,2-oxazole-4-carboxamide
-
3-(2-chlorophenyl)-N-(4-isopropylphenyl-5-methylisoxazole-4-carboxamide)
i.e. UK-106051, a drug-like UPPs inhibitor, UK-106051 binds to a site in the middle of this binding cleft. Substrates FPP and IPP cannot bind simultaneously to UPPs-inhibitor binary complexes
3-(4-chlorophenyl)-N'-[(2,4-dihydroxyphenyl)methyl]-1H-pyrazole-5-carbohydrazide
-
3-(dibenzo[b,d]furan-4-yl)-1-(2,2-diphosphonoethyl)pyridin-1-ium
-
3-([1,1'-biphenyl]-2-yl)-1-(2,2-diphosphonoethyl)pyridin-1-ium
-
3-([1,1'-biphenyl]-3-yl)-1-(2,2-diphosphonoethyl)pyridin-1-ium
-
3-([1,1'-biphenyl]-3-yl)-1-(2-hydroxy-2,2-diphosphonoethyl)pyridin-1-ium
-
3-([1,1'-biphenyl]-4-yl)-1-(2,2-diphosphonoethyl)pyridin-1-ium
-
3-desmethyl (2Z,6E,10E)-geranylgeranyl diphosphate
-
mixed inhibition with farnesyl diphosphate, competitive inhibition with reaction intermediate (2Z,6E,10E)-geranylgeranyl diphosphate
3-desmethyl farnesyl diphosphate
-
competitive with farnesyl diphosphate, mixed inhibition with reaction intermediate (2Z,6E,10E)-geranylgeranyl diphosphate; competitive with farnesyl diphosphate, mixed inhibition with reaction intermediate (Z,E,E)-geranylgeranyl diphosphate
3-desmethyl Z-geranylgeranyl diphosphate
-
mixed inhibition with farnesyl diphosphate, competitive inhibition with reaction intermediate (Z,E,E)-geranylgeranyl diphosphate
3-ethoxy-2-methyl-6-(4-phenyl-1H-pyrazol-3-yl)phenol
-
3-fluoro-1-(2-hydroxy-2,2-diphosphonoethyl)pyridin-1-ium
-
3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-1-(2,2-diphosphonoethyl)pyridin-1-ium
-
3-[(5Z)-5-[[5-(2,4-dichlorophenyl)furan-2-yl]methylidene]-4-oxo-2-sulfanylidene-1,3-thiazolidin-3-yl]propanoic acid
-
3-[3-(hexyloxy)benzamido]-4-hydroxybenzoic acid
-
-
3-[3-(octyloxy)benzamido]benzene-1,2-dicarboxylic acid
-
-
3-[5-(5,6-dihydrobenzimidazo[1,2-c]quinazolin-6-yl)-2,5-dihydrofuran-2-yl]benzenesulfonamide
4,4'-[pentane-1,5-diylbis(oxy)]dibenzenecarboximidamide
4,5-difluoro-2-[3-(octyloxy)benzamido]benzoic acid
-
-
4-(benzyloxy)-3-bromo-5-methoxy-N-[(oxolan-2-yl)methyl]benzamide
-
4-(dibenzo[b,d]furan-4-yl)-1-(2-hydroxy-2,2-diphosphonoethyl)pyridin-1-ium
-
4-([1,1'-biphenyl]-3-yl)-1-(2,2-diphosphonoethyl)pyridin-1-ium
-
4-([1,1'-biphenyl]-4-yl)-1-(2,2-diphosphonoethyl)pyridin-1-ium
-
4-[3-(octyloxy)benzamido]benzene-1,3-dicarboxylic acid
-
-
4-[3-[([1,1'-biphenyl]-4-carbonyl)amino]phenoxy]benzene-1,2-dicarboxylic acid
-
5-(diethoxyphosphoryl)-2-[3-(octyloxy)benzamido]benzoic acid
-
-
5-(methanesulfonyl)-2-[3-(octyloxy)benzamido]benzoic acid
-
-
5-([5-[(1H-benzimidazol-2-yl)sulfanyl]furan-2-yl]methylidene)-1,3-diazinane-2,4,6-trione
-
5-benzyl-3-[1-(4-cyclohexylanilino)ethenyl]-4-hydroxy-5,6-dihydropyridin-2(1H)-one
-
5-benzyl-4-hydroxy-3-[1-[4-(1H-imidazol-1-yl)anilino]ethenyl]-5,6-dihydropyridin-2(1H)-one
-
5-benzyl-4-hydroxy-5-methyl-2-oxo-2,5-dihydro-1H-pyrrole-3-carboxylic acid (4-cyclohexylphenyl)amide
-
-
5-benzyl-N-(4-cyclohexylphenyl)-4-hydroxy-2-oxo-2,5-dihydro-1H-pyrrole-3-carboxamide
-
5-benzyl-N-[4-(hexyloxy)phenyl]-4-hydroxy-2-oxo-2,5-dihydro-1H-pyrrole-3-carboxamide
-
5-bromo-2-[3-(3,3-dimethylbut-1-yn-1-yl)benzamido]benzoic acid
-
-
5-bromo-2-[3-(octyloxy)benzamido]benzoic acid
-
-
5-bromo-2-[4-(6-chloro-2,3-dihydro-1H-indole-1-sulfonyl)benzamido]benzoic acid
-
5-chloro-2-[3-(octyloxy)benzamido]benzoic acid
-
-
5-fluoro-2-[3-(hexyloxy)benzamido]benzoic acid
-
-
5-fluoro-2-[3-(octyloxy)benzamido]benzoic acid
-
-
5-hydroxy-2-[3-(octyloxy)benzamido]benzoic acid
-
-
5-methoxy-2-[3-(octyloxy)benzamido]benzoic acid
-
-
5-methoxy-4-(2-methoxyethoxy)-2-[3-(octyloxy)benzamido]benzoic acid
-
-
5-[3-(octyloxy)benzamido]benzene-1,3-dicarboxylic acid
-
-
6-benzyl-4-hydroxy-3-[1-[4-(1H-imidazol-1-yl)anilino]ethenyl]-5,6-dihydropyridin-2(1H)-one
-
7-(2,6-dimethyl-8-diphospho-2,6-octadienyloxy)-8-methyl-4-trifluoromethyl-chromen-2-one geranyl diphosphate
-
competitive inhibitor, substrate analogue prepared and utilized to study ligand interactions
7-(azepan-1-yl)-3-(4-fluorophenyl)-5-methylpyrazolo[1,5-a]pyrimidine
-
diphosphate
product inhibition
isopentyl thiodiphosphate
-
substrate analogue
Mg2+
inhibitory at high concentrations of e.g. 50 mM; inhibitory at high concentrations of e.g. 50 mM
N,N'-bis(6-chloro-1,3-benzothiazol-2-yl)methanedisulfonamide
N-(2-carbamoylphenyl)-3-(octyloxy)benzamide
-
-
N-(3,5-dichlorophenyl)-4-hydroxy-2-oxo-5-phenyl-2,5-dihydro-1H-pyrrole-3-carboxamide
-
N-(3-amino-3-oxopropyl)-1-benzyl-5-(4-methylphenyl)-N-([4-[(propan-2-yl)oxy]phenyl]methyl)-1H-pyrazole-4-carboxamide
-
-
N-(3-amino-3-oxopropyl)-5-(1-benzothiophen-5-yl)-1-benzyl-N-([4-[(propan-2-yl)oxy]phenyl]methyl)-1H-pyrazole-4-carboxamide
-
-
N-(4-bromo-2-carbamoylphenyl)-3,4-bis(2-methoxyethoxy)benzamide
-
-
N-(4-chlorophenyl)-4-hydroxy-2-oxo-5-phenyl-2,5-dihydro-1H-pyrrole-3-carboxamide
-
N-(4-cyclohexylphenyl)-4-hydroxy-2-oxo-2,5-dihydro-1H-pyrrole-3-carboxamide
-
N-(4-cyclohexylphenyl)-4-hydroxy-2-oxo-5-(2-phenylethyl)-2,5-dihydro-1H-pyrrole-3-carboxamide
-
N-(4-cyclohexylphenyl)-4-hydroxy-2-oxo-5-phenyl-2,5-dihydro-1H-pyrrole-3-carboxamide
-
N-([1,1'-biphenyl]-4-yl)-4-hydroxy-2-oxo-5-(2-phenylethyl)-2,5-dihydro-1H-pyrrole-3-carboxamide
-
N-[2-[(2-[1-[(3-amino-3-iminopropyl)amino]ethenyl]-1-methyl-1H-imidazol-4-yl)acetyl]-1-methyl-1H-imidazol-4-yl]-N2-(diaminomethylidene)glycinamide
N-[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-N'-(3-phenoxyphenyl)biphenyl-4,4'-dicarboxamide
N-[3-[3,4-di(prop-1-en-2-yl)phenoxy]phenyl][1,1'-biphenyl]-4-carboxamide
-
N1,N4-bis(4-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-2-nitroterephthalamide
N1,N4-bis(4-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-2-nitroterephthalamide dihydrochloride
N1,N4-bis[4-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-2-nitrobenzene-1,4-dicarboxamide
-
N4,N4'-bis(3-(1,4,5,6-tetrahydropyrimidin-2-yl)phenyl)biphenyl-4,4'-dicarboxamide
N4,N4'-bis(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-[1,1'-biphenyl]-4,4'-bicarboxamide
N4,N4'-bis(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)biphenyl-4,4'-bis(carbothioamide)
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'- (3-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)phenyl)-[1,1-biphenyl]-4,4-dicarboxamide
-
-
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-(3-(hexyloxy)-phenyl)-[1,1-biphenyl]-4,4-dicarboxamide
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-(3-methoxyphenyl)-[1,1-biphenyl]-4,4-dicarboxamide
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-3(-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)phenyl)-[1,1-biphenyl]-4,4-dicarboxamide
-
NH4+
-
20 mM, 20% inhibition
S-farnesyl thiodiphosphate
-
-
SO42-
competitive inhibitor with respect to (2E,6E)-farnesyl diphosphate; competitive inhibitor with respect to farnesyl diphosphate
viridicatumtoxin B
the inhibitor shows antimicrobial activity, particularly against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA), via inhibition of the UPP synthase activity
[(5Z)-5-[[5-(4-chloro-2-methylphenyl)furan-2-yl]methylidene]-4-oxo-2-sulfanylidene-1,3-thiazolidin-3-yl]acetic acid
-
[1-hydroxy-2-(3-[3-[3-(2-hydroxy-2,2-bis-phosphono-ethyl)-biphenyl-3-ylsulfamoyl]-benzenesulfonylamino]-biphenyl-3-yl)-1-phosphono-ethyl]-phosphonic acid
[1-hydroxy-2-(pyridin-3-yl)ethane-1,1-diyl]bis(phosphonic acid)
-
[1-hydroxy-2-([1(1),2(1):2(3),3(1)-terphenyl]-1(3)-yl)ethane-1,1-diyl]bis(phosphonic acid)
-
[1-hydroxy-2-([1(1),2(1):2(3),3(1)-terphenyl]-1(4)-yl)ethane-1,1-diyl]bis(phosphonic acid)
-
-
[1-hydroxy-2-([1(1),2(1):2(4),3(1)-terphenyl]-1(3)-yl)ethane-1,1-diyl]bis(phosphonic acid)
-
[1-hydroxy-2-[([1(1),2(1):2(3),3(1)-terphenyl]-1(3)-yl)oxy]ethane-1,1-diyl]bis(phosphonic acid)
-
[1-hydroxy-3-([1(1),2(1):2(2),3(1)-terphenyl]-1(3)-yl)propane-1,1-diyl]bis(phosphonic acid)
-
[1-hydroxy-3-([1(1),2(1):2(3),3(1)-terphenyl]-1(3)-yl)propane-1,1-diyl]bis(phosphonic acid)
-
[1-hydroxy-3-([1(1),2(1):2(4),3(1)-terphenyl]-1(3)-yl)propane-1,1-diyl]bis(phosphonic acid)
-
[2-([1,1'-biphenyl]-3-yl)-1-hydroxyethane-1,1-diyl]bis(phosphonic acid)
-
[2-[3-(dibenzo[b,d]furan-4-yl)phenyl]-1-hydroxy-1,1-ethanediyl]bis(phosphonic acid)
i.e. BPH-629. A pharmacophore model is designed on a known bisphosphonate BPH-629 and used to prepare an enriched compound library that was further docked into UppS conformational ensemble generated by molecular dynamics experiment. The docking results in three anthranilic acid derivatives with promising inhibitory activity against the enzyme
[2-[3-(dibenzo[b,d]furan-4-yl)phenyl]-1-hydroxyethane-1,1-diyl]bis(phosphonic acid)
-
[2-[3-(octyloxy)benzamido]phenyl]phosphonic acid
-
-
[3-(3,4-dichlorophenyl)-4,5-dihydro-1,2-oxazole-5,5-diyl]bis(phosphonic acid)
-
[3-[4-(dibenzo[b,d]furan-4-yl)phenyl]-1-hydroxypropane-1,1-diyl]bis(phosphonic acid)
-
[4-([1,1'-biphenyl]-4-yl)butane-1,1-diyl]bis(phosphonic acid)
-
[hydroxy([1(1),2(1):2(3),3(1)-terphenyl]-1(3)-yl)methylene]bis(phosphonic acid)
-
[[(9-ethyl-9H-carbazol-3-yl)amino](hydroxy)methylene]bis(phosphonic acid)
-
(2E,6E)-farnesyl thiodiphosphate
-
(2E,6E)-farnesyl diphosphate analogue
(2E,6E)-farnesyl thiodiphosphate
-
substrate analogue
(2S,3R,4S,5S,6R)-2-(3-((4-((3-(4,5-dihydro-1H-imidazol-2-yl)-phenyl)ethynyl)phenyl)ethynyl)phenoxy)-6-(hydroxymethyl)-tetrahydro-2H-pyran-3,4,5-triol
-
(2S,3R,4S,5S,6R)-2-(3-((4-((3-(4,5-dihydro-1H-imidazol-2-yl)-phenyl)ethynyl)phenyl)ethynyl)phenoxy)-6-(hydroxymethyl)-tetrahydro-2H-pyran-3,4,5-triol
-
-
1,2-Cyclohexanedione
-
1,4-bis(6-(1,4,5,6-tetrahydropyrimidin-2-yl)-1H-indol-2-yl)-benzene
-
1,4-bis(6-(1,4,5,6-tetrahydropyrimidin-2-yl)-1H-indol-2-yl)-benzene
-
-
1,4-bis(6-(4,5-dihydro-1H-imidazol-2-yl)-1H-indol-2-yl)benzene
-
1,4-bis(6-(4,5-dihydro-1H-imidazol-2-yl)-1H-indol-2-yl)benzene
-
-
2,2'-(5,5'-(1,3-phenylenebis(ethyne-2,1-diyl))bis(3-bromo-5,1-phenylene))diethanamine
-
2,2'-(5,5'-(1,3-phenylenebis(ethyne-2,1-diyl))bis(3-bromo-5,1-phenylene))diethanamine
-
-
2,2'-(E)-ethene-1,2-diylbis[6-(4,5-dihydro-1H-imidazol-2-yl)-1H-indole]
-
2,2'-(E)-ethene-1,2-diylbis[6-(4,5-dihydro-1H-imidazol-2-yl)-1H-indole]
-
-
2-propanol
-
3-[5-(5,6-dihydrobenzimidazo[1,2-c]quinazolin-6-yl)-2,5-dihydrofuran-2-yl]benzenesulfonamide
barely inhibits UPPS of Escherichia coli; barely inhibits UPPS of Escherichia coli; i.e. HTS04781. contrary to Helicobacter pylori, HTS04781 is almost not inhibitory in Escherichia coli
3-[5-(5,6-dihydrobenzimidazo[1,2-c]quinazolin-6-yl)-2,5-dihydrofuran-2-yl]benzenesulfonamide
i.e. HTS04781, crystallization data. The sulfonamide group forms H-bonds with Gly16 and Arg26 and the N atom in the tetracyclic ring is hydrogen bound to the main chain of Met12. Extensive hydrophobic interactions are found between the tetracyclic ring with the surrounding residues including Met12, His30, Gly33 and Val34
4,4'-[pentane-1,5-diylbis(oxy)]dibenzenecarboximidamide
-
4,4'-[pentane-1,5-diylbis(oxy)]dibenzenecarboximidamide
-
-
BPH-629
-
potent inhibitor
BPH-629
-
bindiing structure in enzyme chain B, overview
Butanedione
-
clomiphene
inhibits the synthesis of undecaprenyl phosphate, antagonizes the activity of targocil
clomiphene
inhibits the synthesis of undecaprenyl phosphate, antagonizes the activity of targocil
EDTA
-
-
farnesyl thiodiphosphate
-
farnesyl thiodiphosphate
-
-
MAC-0547630
the inhibitor shows great promise as a whole cell-active probe due to its selective and potent inhibition of undecaprenyl diphosphate synthase
MAC-0547630
the inhibitor shows great promise as a whole cell-active probe due to its selective and potent inhibition of undecaprenyl diphosphate synthase
MAC-0547630
the inhibitor shows great promise as a whole cell-active probe due to its selective and potent inhibition of undecaprenyl diphosphate synthase
MAC-0557874
-
MAC-0588238
-
MAC-0602693
-
N,N'-bis(6-chloro-1,3-benzothiazol-2-yl)methanedisulfonamide
barely inhibits UPPS of Escherichia coli; i.e. BTB06061
N,N'-bis(6-chloro-1,3-benzothiazol-2-yl)methanedisulfonamide
i.e. BTB06061, crystallization data. The sulfur atom in the thiazole ring of BTB06061 may form H-bonds with Asn15 and His30 while the SO2 group is hydrogen bound with Met12. The aromatic rings of BTB06061 form hydrophobic interactions with the surrounding hydrophobic residues, including Val34, Leu37, Ala56 and Tyr79
N-[2-[(2-[1-[(3-amino-3-iminopropyl)amino]ethenyl]-1-methyl-1H-imidazol-4-yl)acetyl]-1-methyl-1H-imidazol-4-yl]-N2-(diaminomethylidene)glycinamide
weak inhibition
N-[2-[(2-[1-[(3-amino-3-iminopropyl)amino]ethenyl]-1-methyl-1H-imidazol-4-yl)acetyl]-1-methyl-1H-imidazol-4-yl]-N2-(diaminomethylidene)glycinamide
-
weak inhibition
N-[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-N'-(3-phenoxyphenyl)biphenyl-4,4'-dicarboxamide
-
N-[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-N'-(3-phenoxyphenyl)biphenyl-4,4'-dicarboxamide
-
-
N1,N4-bis(4-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-2-nitroterephthalamide
-
N1,N4-bis(4-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-2-nitroterephthalamide
-
-
N1,N4-bis(4-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-2-nitroterephthalamide dihydrochloride
-
N1,N4-bis(4-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-2-nitroterephthalamide dihydrochloride
-
-
N4,N4'-bis(3-(1,4,5,6-tetrahydropyrimidin-2-yl)phenyl)biphenyl-4,4'-dicarboxamide
-
N4,N4'-bis(3-(1,4,5,6-tetrahydropyrimidin-2-yl)phenyl)biphenyl-4,4'-dicarboxamide
-
-
N4,N4'-bis(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-[1,1'-biphenyl]-4,4'-bicarboxamide
i.e. BPH-1358
N4,N4'-bis(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-[1,1'-biphenyl]-4,4'-bicarboxamide
-
i.e. BPH-1358
N4,N4'-bis(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)biphenyl-4,4'-bis(carbothioamide)
-
N4,N4'-bis(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)biphenyl-4,4'-bis(carbothioamide)
-
-
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-(3-(hexyloxy)-phenyl)-[1,1-biphenyl]-4,4-dicarboxamide
-
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-(3-(hexyloxy)-phenyl)-[1,1-biphenyl]-4,4-dicarboxamide
-
-
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-(3-methoxyphenyl)-[1,1-biphenyl]-4,4-dicarboxamide
-
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-(3-methoxyphenyl)-[1,1-biphenyl]-4,4-dicarboxamide
-
-
Phenylglyoxal
-
spirohexaline
-
-
spirohexaline
the molecule shows weak inhibitory activity against catalytically related enzymes such as bacterial octaprenyl pyrophosphate synthase and yeast dehydrodolichyl pyrophosphate synthase, indicating that the compound preferentially inhibits UPP synthase. The inhibitor shows antimicrobial activity, particularly against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA), via inhibition of the UPP synthase activity. Molecular docking and homology modeling of UPP synthase-spirohexaline complex
targocil
-
tetramic acid
-
the crystal structure is used for modelling with bound inhibitor tetramic acid
tetramic acid
binding structure, overview
Triton X-100
-
C55 is retained in the active site for further elongation, whereas the kcat is increased by 190fold under steady-state condition by 0.1% Triton X-100
Triton X-100
-
inhibition at concentration above 1%
Triton X-100
-
marked decrease of activity when a small amount of detergent is added, activity gradually increases as further detergent is added, being fully restored when the concentration reaches 2%
viridicatumtoxin
-
-
viridicatumtoxin
originally isolated from Penicillium viridicatum, the molecule shows weak inhibitory activity against catalytically related enzymes such as bacterial octaprenyl pyrophosphate synthase and yeast dehydrodolichyl pyrophosphate synthase, indicating that the compound preferentially inhibits UPP synthase. The inhibitor shows antimicrobial activity, particularly against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA). Molecular modeling strongly suggests that the hydrophobic spirobicyclic ring of viridicatumtoxin interacts with three hydrophobic clefts of the active site in MRSA UPP synthase
[1-hydroxy-2-(3-[3-[3-(2-hydroxy-2,2-bis-phosphono-ethyl)-biphenyl-3-ylsulfamoyl]-benzenesulfonylamino]-biphenyl-3-yl)-1-phosphono-ethyl]-phosphonic acid
-
[1-hydroxy-2-(3-[3-[3-(2-hydroxy-2,2-bis-phosphono-ethyl)-biphenyl-3-ylsulfamoyl]-benzenesulfonylamino]-biphenyl-3-yl)-1-phosphono-ethyl]-phosphonic acid
-
-
additional information
-
Z-isomer ZHIPP, (Z)-3-methyl-3-pentenyl diphosphate, which is not accepted as substrate by the enzyme, exhibited no inhibition on the synthase reaction
-
additional information
a small-molecule screening platform for the discovery of inhibitors of undecaprenyl diphosphate synthase; small-molecule screening platform for the discovery of inhibitors of undecaprenyl diphosphate synthase
-
additional information
-
farnesol (1 mM) lacking the diphosphate does not inhibit the UPPS reaction; no inhibition by farnesol
-
additional information
screening of inhibitory activities of a series of amidine and bisamidine compounds against Staphylococcus aureus and Escherichia coli, overview
-
additional information
-
screening of inhibitory activities of a series of amidine and bisamidine compounds against Staphylococcus aureus and Escherichia coli, overview
-
additional information
antagonistic interactions in high-throughput screening and in compound mode of action studies. MIC values and fractional inhibitory concentrations of combinations of clomiphene and antibiotics against strain CA-MRSA USA 300, overview
-
additional information
-
a small-molecule screening platform for the discovery of inhibitors of undecaprenyl diphosphate synthase
-
additional information
a small-molecule screening platform for the discovery of inhibitors of undecaprenyl diphosphate synthase
-
additional information
-
virtual screening of inhibitors from a library of 58635 compounds performed
-
additional information
virtual screening of inhibitors from a library of 58635 compounds performed
-
additional information
-
no inhibition by phosphate
-
additional information
-
no inhibition by phosphate
-
additional information
the inhibitors tested show antimicrobial, cytotoxic, and enzyme inhibitory activities, overview
-
additional information
-
the inhibitors tested show antimicrobial, cytotoxic, and enzyme inhibitory activities, overview
-
additional information
-
screening of inhibitory activities of a series of amidine and bisamidine compounds against Staphylococcus aureus and Escherichia coli, overview
-
additional information
antagonistic interactions in high-throughput screening and in compound mode of action studies. MIC values and fractional inhibitory concentrations of combinations of clomiphene and antibiotics against strain CA-MRSA USA 300, overview
-
additional information
-
antagonistic interactions in high-throughput screening and in compound mode of action studies. MIC values and fractional inhibitory concentrations of combinations of clomiphene and antibiotics against strain CA-MRSA USA 300, overview
-
additional information
-
a small-molecule screening platform for the discovery of inhibitors of undecaprenyl diphosphate synthase
-
additional information
a small-molecule screening platform for the discovery of inhibitors of undecaprenyl diphosphate synthase
-
additional information
-
tetramic acids are selective and potent inhibitors of UPPS
-
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0.0011 - 0.0015
(2E,6E)-8-(N-methyl-2-aminobenzoyloxy)-3,7-dimethyl-2,6-octandien-1-diphosphate
-
pH 7.5, 25°C
0.00011 - 18
(2E,6E)-farnesyl diphosphate
0.0088 - 0.064
(2E,6E,10E)-geranylgeranyl diphosphate
0.0043 - 18.1
(2Z,6E,10E)-geranylgeranyl diphosphate
0.0588
(2Z,6E,10E,14E)-farnesylgeranyl diphosphate
-
pH 8.5, 37°C
0.0526
(2Z,6Z,10E,14E)-farnesylgeranyl diphosphate
-
pH 8.5, 37°C
0.00017
(E,E)-(2-Diazo-3-trifluoropropionyloxy)geranyl diphosphate
-
pH 7.5, 35°C
0.00013 - 0.103
(E,E)-farnesyl diphosphate
0.008 - 0.037
(E,E,E)-geranylgeranyl diphosphate
0.0189 - 0.305
(Z,E)-farnesyl diphosphate
0.0023 - 0.134
(Z,E,E)-Geranylgeranyl diphosphate
0.0588
(Z,E,E,E)-farnesylgeranyl diphosphate
-
pH 8.5, 37°C
0.0526
(Z,Z,E,E)-farnesylgeranyl diphosphate
-
pH 8.5, 37°C
0.00069
7-(2,6-dimethyl-8-diphospho-2,6-octadienyloxy)-8-methyl-4-trifluoromethyl-chromen-2-one geranyl diphosphate
-
alternative substrate, pH 7.5, 25°C
0.00011 - 0.155
farnesyl diphosphate
0.0032 - 0.036
geranyl diphosphate
0.0003 - 0.0075
geranylgeranyl diphosphate
0.00041 - 252.5
isopentenyl diphosphate
0.0267 - 0.032
neryl diphosphate
additional information
additional information
-
0.00011
(2E,6E)-farnesyl diphosphate
wild-type
0.00011
(2E,6E)-farnesyl diphosphate
wild type, pH 7.5, temperature not specified in the publication
0.00013
(2E,6E)-farnesyl diphosphate
pH 7.5, 35°C
0.00015
(2E,6E)-farnesyl diphosphate
mutant C234A
0.00015
(2E,6E)-farnesyl diphosphate
mutant C234A, pH 7.5, temperature not specified in the publication
0.00017
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, S83(Ala)5 mutant
0.00028
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, H199A mutant
0.00037
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, wild type with 1 mM MgCl2
0.0004
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C
0.0004
(2E,6E)-farnesyl diphosphate
-
wild-type enzyme, pH 7.5, 25°C
0.0004
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, wild type
0.0004
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, wild type
0.0004
(2E,6E)-farnesyl diphosphate
-
mutant enzyme W91F, pH 7.5, 25°C, wild-type enzyme, pH 7.5, 25°C
0.0004
(2E,6E)-farnesyl diphosphate
-
mutant enzymes E73A, D150A and D218A, pH 7.5, 25°C; wild-type enzyme, pH 7.5, 25°C
0.0004
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, E73A mutant
0.0004
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, E81A mutant
0.0004
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, N74A mutant
0.0004
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, S83(Ala)1 mutant
0.0004
(2E,6E)-farnesyl diphosphate
-
wild type, 25°C, pH 7.5
0.00044
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, wild type with 3 mM MgCl2
0.00046
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, D26K mutant with 0.5 mM MgCl2
0.0005
(2E,6E)-farnesyl diphosphate
-
mutant enzyme W221F, pH 7.5, 25°C
0.0005
(2E,6E)-farnesyl diphosphate
-
mutant enzyme D26A, pH 7.5, 25°C
0.0005
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, D26A mutant
0.0005
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, DELTAS72 mutant
0.00055
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, D26R mutant with 0.5 mM MgCl2
0.00066
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, H199A/E213A mutant
0.00067
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, D26E mutant with 0.5 mM MgCl2
0.0007
(2E,6E)-farnesyl diphosphate
-
mutant enzyme E213A, pH 7.5, 25°C
0.0007
(2E,6E)-farnesyl diphosphate
-
mutant enzyme W149F, pH 7.5, 25°C
0.0007
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, E213A mutant
0.001
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, DELTAS83 mutant
0.001
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, S71A mutant
0.0015
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, wild type with 0.05 mM MgCl2
0.0015
(2E,6E)-farnesyl diphosphate
pH 7.5, 30°C, W210A mutant
0.00157
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, wild type with 50 mM MgCl2
0.0016
(2E,6E)-farnesyl diphosphate
-
L85A mutant, 25°C, pH 7.5
0.0016
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, R77A mutant
0.0018
(2E,6E)-farnesyl diphosphate
-
mutant enzyme W75F, pH 7.5, 25°C
0.0018
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, DELTAV82S83 mutant
0.002
(2E,6E)-farnesyl diphosphate
-
mutant enzyme W31F, pH 7.5, 25°C
0.0021
(2E,6E)-farnesyl diphosphate
-
L88A mutant, 25°C, pH 7.5
0.0023
(2E,6E)-farnesyl diphosphate
E76Q mutant, pH 7.5, 37°C
0.0026
(2E,6E)-farnesyl diphosphate
-
F89A mutant, 25°C, pH 7.5
0.0028
(2E,6E)-farnesyl diphosphate
pH 7.5, 30°C, Y148F mutant
0.003
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, H43A mutant
0.0032
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, W75A mutant
0.0033
(2E,6E)-farnesyl diphosphate
G32R mutant, pH 7.5, 37°C
0.0034
(2E,6E)-farnesyl diphosphate
-
mutant enzyme W207F, pH 7.5, 25°C
0.0036
(2E,6E)-farnesyl diphosphate
wild-type, pH 7.5, 37°C
0.0049
(2E,6E)-farnesyl diphosphate
R42G mutant, pH 7.5, 37°C
0.005
(2E,6E)-farnesyl diphosphate
G32R/R42G mutant, pH 7.5, 37°C
0.0077
(2E,6E)-farnesyl diphosphate
pH 7.5, 30°C, Y71S mutant
0.0083
(2E,6E)-farnesyl diphosphate
wild-type, pH 7.5, 37°C
0.0083
(2E,6E)-farnesyl diphosphate
pH 7.5, 30°C, wild type
0.0091
(2E,6E)-farnesyl diphosphate
-
pH 8.5, 37°C
0.0092
(2E,6E)-farnesyl diphosphate
wild type, pH 7.5, temperature not specified in the publication
0.0096
(2E,6E)-farnesyl diphosphate
mutant C234A, pH 7.5, temperature not specified in the publication
0.0104
(2E,6E)-farnesyl diphosphate
mutant enzyme N77Q, pH 7.5, 37°C
0.0114
(2E,6E)-farnesyl diphosphate
E84Q mutant, pH 7.5, 37°C
0.01213
(2E,6E)-farnesyl diphosphate
pH 7.5, 30°C, W224A mutant
0.0139
(2E,6E)-farnesyl diphosphate
mutant enzyme N77D, pH 7.5, 37°C
0.0147
(2E,6E)-farnesyl diphosphate
mutant enzyme N77A, pH 7.5, 37°C
0.0222
(2E,6E)-farnesyl diphosphate
pH 7.5, 30°C, F207S mutant
0.0231
(2E,6E)-farnesyl diphosphate
R80A mutant, pH 7.5, 37°C
0.0332
(2E,6E)-farnesyl diphosphate
D29A mutant, pH 7.5, 37°C
0.0495
(2E,6E)-farnesyl diphosphate
mutant enzyme W78I, pH 7.5, 37°C
0.1207
(2E,6E)-farnesyl diphosphate
mutant enzyme W78R, pH 7.5, 37°C
0.144
(2E,6E)-farnesyl diphosphate
R33A mutant, pH 7.5, 37°C
0.155
(2E,6E)-farnesyl diphosphate
mutant enzyme N78D, pH 7.5, 37°C
3.5
(2E,6E)-farnesyl diphosphate
D221A mutant, pH 7.3, 37°C
5.4
(2E,6E)-farnesyl diphosphate
E216Q mutant, pH 7.3, 37°C
6.6
(2E,6E)-farnesyl diphosphate
E193Q mutant, pH 7.3, 37°C
8.3
(2E,6E)-farnesyl diphosphate
wild type, pH 7.3, 37°C
9.2
(2E,6E)-farnesyl diphosphate
F73A mutant, pH 7.3, 37°C
9.6
(2E,6E)-farnesyl diphosphate
S74A mutant, pH 7.3, 37°C
11.2
(2E,6E)-farnesyl diphosphate
D226A mutant, pH 7.3, 37°C
11.6
(2E,6E)-farnesyl diphosphate
E201Q mutant, pH 7.3, 37°C
13.6
(2E,6E)-farnesyl diphosphate
R203S mutant, pH 7.3, 37°C
18
(2E,6E)-farnesyl diphosphate
R197S mutant, pH 7.3, 37°C
0.0088
(2E,6E,10E)-geranylgeranyl diphosphate
wild-type, pH 7.5, 37°C
0.0093
(2E,6E,10E)-geranylgeranyl diphosphate
-
pH 8.5, 37°C
0.064
(2E,6E,10E)-geranylgeranyl diphosphate
R33A mutant, pH 7.5, 37°C
0.0043
(2Z,6E,10E)-geranylgeranyl diphosphate
G32R mutant, pH 7.5, 37°C
0.0045
(2Z,6E,10E)-geranylgeranyl diphosphate
R42G mutant, pH 7.5, 37°C
0.005
(2Z,6E,10E)-geranylgeranyl diphosphate
mutant enzyme N77Q, pH 7.5, 37°C
0.005
(2Z,6E,10E)-geranylgeranyl diphosphate
D29A mutant, pH 7.5, 37°C
0.0059
(2Z,6E,10E)-geranylgeranyl diphosphate
G32R-R42G mutant, pH 7.5, 37°C
0.006
(2Z,6E,10E)-geranylgeranyl diphosphate
E84Q mutant, pH 7.5, 37°C
0.0061
(2Z,6E,10E)-geranylgeranyl diphosphate
R80A mutant, pH 7.5, 37°C
0.008
(2Z,6E,10E)-geranylgeranyl diphosphate
mutant enzyme N77D, pH 7.5, 37°C; mutant enzyme W78D, pH 7.5, 37°C
0.0082
(2Z,6E,10E)-geranylgeranyl diphosphate
wild-type enzyme, pH 7.5, 37°C
0.0082
(2Z,6E,10E)-geranylgeranyl diphosphate
wild-type, pH 7.5, 37°C
0.0089
(2Z,6E,10E)-geranylgeranyl diphosphate
-
37°C, pH 37°C
0.0095
(2Z,6E,10E)-geranylgeranyl diphosphate
R33A mutant, pH 7.5, 37°C
0.0102
(2Z,6E,10E)-geranylgeranyl diphosphate
mutant enzyme N77A, pH 7.5, 37°C
0.015
(2Z,6E,10E)-geranylgeranyl diphosphate
mutant enzyme W78R, pH 7.5, 37°C
0.0154
(2Z,6E,10E)-geranylgeranyl diphosphate
-
pH 8.5, 37°C
0.019
(2Z,6E,10E)-geranylgeranyl diphosphate
mutant enzyme W78I, pH 7.5, 37°C
0.023
(2Z,6E,10E)-geranylgeranyl diphosphate
E76Q mutant, pH 7.5, 37°C
5.3
(2Z,6E,10E)-geranylgeranyl diphosphate
S74A mutant, pH 7.3, 37°C
5.8
(2Z,6E,10E)-geranylgeranyl diphosphate
E216Q mutant, pH 7.3, 37°C
6
(2Z,6E,10E)-geranylgeranyl diphosphate
R197S mutant, pH 7.3, 37°C
7.5
(2Z,6E,10E)-geranylgeranyl diphosphate
D226A mutant, pH 7.3, 37°C
8.1
(2Z,6E,10E)-geranylgeranyl diphosphate
E193Q mutant, pH 7.3, 37°C
8.1
(2Z,6E,10E)-geranylgeranyl diphosphate
R203S mutant, pH 7.3, 37°C
8.2
(2Z,6E,10E)-geranylgeranyl diphosphate
wild type, pH 7.3, 37°C
9.4
(2Z,6E,10E)-geranylgeranyl diphosphate
F73A mutant, pH 7.3, 37°C
17.7
(2Z,6E,10E)-geranylgeranyl diphosphate
E201Q mutant, pH 7.3, 37°C
18.1
(2Z,6E,10E)-geranylgeranyl diphosphate
D221A mutant, pH 7.3, 37°C
0.00013
(E,E)-farnesyl diphosphate
pH 7.5, 35°C
0.00013
(E,E)-farnesyl diphosphate
-
pH 7.5, 35°C
0.007
(E,E)-farnesyl diphosphate
-
pH 7.4, 35°C
0.008
(E,E)-farnesyl diphosphate
-
-
0.0091
(E,E)-farnesyl diphosphate
-
-
0.0091
(E,E)-farnesyl diphosphate
-
pH 8.5, 37°C
0.103
(E,E)-farnesyl diphosphate
-
pH 7.5, 35°C
0.008
(E,E,E)-geranylgeranyl diphosphate
-
pH 7.5, 35°C
0.0093
(E,E,E)-geranylgeranyl diphosphate
-
pH 8.5, 37°C
0.037
(E,E,E)-geranylgeranyl diphosphate
-
pH 7.5, 35°C
0.0189
(Z,E)-farnesyl diphosphate
-
pH 8.5, 37°C
0.305
(Z,E)-farnesyl diphosphate
-
pH 7.5, 35°C
0.0023
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme E76Q, pH 7.5, 37°C
0.0043
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme G32R, pH 7.5, 37°C
0.0045
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme R42G, pH 7.5, 37°C
0.005
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme N77Q, pH 7.5, 37°C
0.005
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme D29A, pH 7.5, 37°C
0.0053
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme S74A, pH 7.3, 37°C
0.0058
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme R216Q, pH 7.3, 37°C
0.0059
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme G32R/R42G, pH 7.5, 37°C
0.006
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme R197S, pH 7.3, 37°C
0.006
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme E84Q, pH 7.5, 37°C
0.0061
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme R80A, pH 7.5, 37°C
0.008
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme N77D, pH 7.5, 37°C
0.008
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme W78D, pH 7.5, 37°C
0.0081
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme E193Q, pH 7.3, 37°C
0.0081
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme R203S, pH 7.3, 37°C
0.0082
(Z,E,E)-Geranylgeranyl diphosphate
wild-type enzyme, pH 7.5, 37°C
0.0082
(Z,E,E)-Geranylgeranyl diphosphate
wild-type enzyme, pH 7.3, 37°C
0.0094
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme F73A, pH 7.3, 37°C
0.0095
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme R33A, pH 7.5, 37°C
0.0102
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme N77A, pH 7.5, 37°C
0.015
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme W78R, pH 7.5, 37°C
0.0154
(Z,E,E)-Geranylgeranyl diphosphate
-
pH 8.5, 37°C
0.0177
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme E201Q, pH 7.3, 37°C
0.0181
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme D221A, pH 7.3, 37°C
0.019
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme W78I, pH 7.5, 37°C
0.134
(Z,E,E)-Geranylgeranyl diphosphate
-
pH 7.5, 35°C
0.00011
farnesyl diphosphate
wild-type, pH 7.5
0.00015
farnesyl diphosphate
mutant C234A, pH 7.5
0.0003
farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H199A
0.0004
farnesyl diphosphate
-
wild-type enzyme, pH 7.5, 25°C
0.0004
farnesyl diphosphate
wild-type enzyme, pH 7.5, 25°C
0.0004
farnesyl diphosphate
-
wild-type enzyme, pH 7.5, 25°C
0.0004
farnesyl diphosphate
-
mutant enzymes E73A, D150A and D218A, pH 7.5, 25°C
0.0004
farnesyl diphosphate
mutant enzyme E73A, N74A and E81A, pH 7.5, 25°C
0.0004
farnesyl diphosphate
-
mutant enzyme W91F, pH 7.5, 25°C
0.0004
farnesyl diphosphate
-
pH 7.5, 25°C, wild-type enzyme
0.0004
farnesyl diphosphate
pH 7.5, 25°C, wild-type enzyme
0.0004
farnesyl diphosphate
-
pH 7.5, 25°C, recombinant wild-type enzyme
0.00046
farnesyl diphosphate
pH 7.5, 25°C, mutant D26K
0.0005
farnesyl diphosphate
-
mutant enzyme W221F, pH 7.5, 25°C
0.0005
farnesyl diphosphate
-
mutant enzyme D26A, pH 7.5, 25°C
0.0005
farnesyl diphosphate
pH 7.5, 25°C, mutant D26A
0.0005
farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant D26A
0.00055
farnesyl diphosphate
pH 7.5, 25°C, mutant D26R
0.00066
farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H199A/E213A
0.00067
farnesyl diphosphate
pH 7.5, 25°C, mutant D26E
0.0007
farnesyl diphosphate
-
pH 7.5, 30°C
0.0007
farnesyl diphosphate
-
mutant enzyme E213A, pH 7.5, 25°C
0.0007
farnesyl diphosphate
-
mutant enzyme W149F, pH 7.5, 25°C
0.0007
farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant E213A
0.001
farnesyl diphosphate
mutant enzyme S71A, pH 7.5, 25°C
0.0015
farnesyl diphosphate
pH 7.5, 30°C, recombinant mutant W210A
0.0016
farnesyl diphosphate
mutant enzyme R77A, pH 7.5, 25°C
0.0016
farnesyl diphosphate
-
pH 7.5, 25°C, mutant L85A
0.0018
farnesyl diphosphate
-
mutant enzyme W75F, pH 7.5, 25°C
0.002
farnesyl diphosphate
-
mutant enzyme W31F, pH 7.5, 25°C
0.0021
farnesyl diphosphate
-
pH 7.5, 25°C, mutant L88A
0.0023
farnesyl diphosphate
mutant enzyme E76Q, pH 7.5, 37°C
0.0026
farnesyl diphosphate
-
pH 7.5, 25°C, mutant F89A
0.0028
farnesyl diphosphate
pH 7.5, 30°C, recombinant mutant Y148F
0.003
farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H43A
0.0032
farnesyl diphosphate
mutant enzyme W75A, pH 7.5, 25°C
0.0033
farnesyl diphosphate
mutant enzyme G32R, pH 7.5, 37°C
0.0034
farnesyl diphosphate
-
mutant enzyme W207F, pH 7.5, 25°C
0.0035
farnesyl diphosphate
mutant enzyme D221A, pH 7.3, 37°C
0.0036
farnesyl diphosphate
wild-type enzyme, pH 7.5, 37°C
0.0049
farnesyl diphosphate
mutant enzyme R42G, pH 7.5, 37°C
0.005
farnesyl diphosphate
mutant enzyme G32R/R42G, pH 7.5, 37°C
0.0054
farnesyl diphosphate
mutant enzyme R216Q, pH 7.3, 37°C
0.0066
farnesyl diphosphate
mutant enzyme E193Q, pH 7.3, 37°C
0.0077
farnesyl diphosphate
pH 7.5, 30°C, recombinant mutant Y71S
0.0083
farnesyl diphosphate
wild type enzyme
0.0083
farnesyl diphosphate
wild-type enzyme, pH 7.3, 37°C
0.0083
farnesyl diphosphate
wild-type, pH 7.5, 37°C
0.0083
farnesyl diphosphate
pH 7.5, 30°C, recombinant wild-type enzyme
0.0092
farnesyl diphosphate
mutant enzyme F73A, pH 7.3, 37°C
0.0093
farnesyl diphosphate
mutant enzyme F73A
0.0096
farnesyl diphosphate
mutant enzyme S74A, pH 7.3, 37°C
0.0104
farnesyl diphosphate
mutant enzyme N77Q, pH 7.5, 37°C
0.0112
farnesyl diphosphate
mutant enzyme D226A, pH 7.3, 37°C
0.0114
farnesyl diphosphate
mutant enzyme E84Q, pH 7.5, 37°C
0.0116
farnesyl diphosphate
mutant enzyme E201Q, pH 7.3, 37°C
0.0136
farnesyl diphosphate
mutant enzyme R203S, pH 7.3, 37°C
0.0139
farnesyl diphosphate
mutant enzyme N77D, pH 7.5, 37°C
0.014
farnesyl diphosphate
mutant enzyme A72L/F73L/W78L
0.0147
farnesyl diphosphate
mutant enzyme N77A, pH 7.5, 37°C
0.018
farnesyl diphosphate
mutant enzyme R197S, pH 7.3, 37°C
0.022
farnesyl diphosphate
pH 7.5, 30°C, recombinant mutant F207S
0.0231
farnesyl diphosphate
mutant enzyme R80A, pH 7.5, 37°C
0.026
farnesyl diphosphate
mutant enzyme A72L/F73L
0.0332
farnesyl diphosphate
mutant enzyme D29A, pH 7.5, 37°C
0.0495
farnesyl diphosphate
mutant enzyme W78I, pH 7.5, 37°C
0.1207
farnesyl diphosphate
mutant enzyme W78R, pH 7.5, 37°C
0.144
farnesyl diphosphate
mutant enzyme R33A, pH 7.5, 37°C
0.155
farnesyl diphosphate
mutant enzyme N78D, pH 7.5, 37°C
0.0032
geranyl diphosphate
-
pH 7.5, 35°C
0.036
geranyl diphosphate
-
25°C, pH 7.5
0.0003
geranylgeranyl diphosphate
-
pH 7.5, 25°C, wild-type enzyme
0.0003
geranylgeranyl diphosphate
-
wild type, 25°C, pH 7.5
0.0007
geranylgeranyl diphosphate
-
pH 7.5, 25°C, mutant L88A
0.0007
geranylgeranyl diphosphate
-
L88A mutant, 25°C, pH 7.5
0.001
geranylgeranyl diphosphate
-
pH 7.5, 25°C, mutant F89A
0.001
geranylgeranyl diphosphate
-
F89A mutant, 25°C, pH 7.5
0.0012
geranylgeranyl diphosphate
-
pH 7.5, 25°C, mutant L85A
0.0012
geranylgeranyl diphosphate
-
L85A mutant, 25°C, pH 7.5
0.0075
geranylgeranyl diphosphate
mutant enzyme D226A, pH 7.3, 37°C
0.00041
isopentenyl diphosphate
pH 7.5, 25°C, wild-type enzyme
0.0009
isopentenyl diphosphate
pH 7.5, 25°C, mutant D26K
0.0014
isopentenyl diphosphate
pH 7.5, 25°C, mutant D26A
0.00192
isopentenyl diphosphate
pH 7.5, 35°C
0.00192
isopentenyl diphosphate
-
pH 7.5, 35°C
0.0021
isopentenyl diphosphate
pH 7.5, 25°C, mutant D26E
0.0022
isopentenyl diphosphate
pH 7.5, 25°C, mutant D26R
0.004
isopentenyl diphosphate
-
pH 7.5, 25°C, recombinant wild-type enzyme
0.0041
isopentenyl diphosphate
-
pH 7.5, 25°C
0.0041
isopentenyl diphosphate
pH 7.5, 25°C
0.0041
isopentenyl diphosphate
-
pH 7.5, 25°C
0.0041
isopentenyl diphosphate
-
wild-type enzyme, pH 7.5, 25°C
0.0041
isopentenyl diphosphate
-
pH 7.5, 25°C, wild type
0.0041
isopentenyl diphosphate
pH 7.5, 25°C, wild type
0.0041
isopentenyl diphosphate
-
pH 7.5, 25°C, wild-type enzyme, with farnesyl diphosphate
0.0041
isopentenyl diphosphate
-
wild type, with (2E,6E)-farnesyl diphosphate, 25°C, pH 7.5
0.0044
isopentenyl diphosphate
-
mutant enzyme D218A, pH 7.5, 25°C
0.0059
isopentenyl diphosphate
-
mutant enzyme W31F, pH 7.5, 25°C
0.0059
isopentenyl diphosphate
mutant enzyme G32R/R42G, pH 7.5, 37°C
0.0059
isopentenyl diphosphate
G32R/R42G mutant, pH 7.5, 37°C
0.006
isopentenyl diphosphate
mutant enzyme G32R, pH 7.5, 37°C
0.006
isopentenyl diphosphate
G32R mutant, pH 7.5, 37°C
0.0072
isopentenyl diphosphate
-
mutant enzyme W91F, pH 7.5, 25°C
0.0078
isopentenyl diphosphate
wild-type enzyme, pH 7.5, 37°C
0.0078
isopentenyl diphosphate
wild-type enzyme, pH 7.3, 37°C
0.0078
isopentenyl diphosphate
wild-type, pH 7.5, 37°C
0.0078
isopentenyl diphosphate
pH 7.5, 30°C, recombinant wild-type enzyme
0.0078
isopentenyl diphosphate
-
pH 7.5, 25°C, S83(Ala)5 mutant
0.0078
isopentenyl diphosphate
pH 7.5, 30°C, wild type
0.008
isopentenyl diphosphate
mutant enzyme N74A, pH 7.5, 25°C
0.008
isopentenyl diphosphate
pH 7.5, 25°C, N74A mutant
0.0085
isopentenyl diphosphate
-
37°C, pH 37°C
0.009
isopentenyl diphosphate
mutant enzyme N77Q, pH 7.5, 37°C
0.0092
isopentenyl diphosphate
wild-type
0.0092
isopentenyl diphosphate
wild-type, pH 7.5
0.0092
isopentenyl diphosphate
pH 7.5, 25°C, D26K mutant with 0.5 mM MgCl2
0.0096
isopentenyl diphosphate
mutant C234A, pH 7.5
0.0096
isopentenyl diphosphate
mutant C234A
0.0097
isopentenyl diphosphate
pH 7.5, 25°C, wild type with 1 mM MgCl2
0.0114
isopentenyl diphosphate
mutant enzyme E76Q, pH 7.5, 37°C
0.0114
isopentenyl diphosphate
E76Q mutant, pH 7.5, 37°C
0.0115
isopentenyl diphosphate
pH 7.5, 25°C, wild type with 0.05 mM MgCl2
0.0117
isopentenyl diphosphate
-
pH 7.5, 25°C, DELTAS72 mutant
0.012
isopentenyl diphosphate
mutant enzyme N77A, pH 7.5, 37°C
0.013
isopentenyl diphosphate
-
pH 7.5, 25°C, wild-type enzyme, with geranylgeranyl diphosphate
0.013
isopentenyl diphosphate
-
wild type, with geranylgeranyl diphosphate, 25°C, pH 7.5
0.0131
isopentenyl diphosphate
mutant enzyme W78D, pH 7.5, 37°C
0.014
isopentenyl diphosphate
-
pH 7.5, 35°C
0.014
isopentenyl diphosphate
-
mutant enzyme W221F, pH 7.5, 25°C
0.014
isopentenyl diphosphate
-
pH 7.5, 25°C, recombinant mutant D26A
0.0141
isopentenyl diphosphate
-
mutant enzyme D26A, pH 7.5, 25°C
0.0141
isopentenyl diphosphate
mutant enzyme W78I, pH 7.5, 37°C
0.0141
isopentenyl diphosphate
-
pH 7.5, 25°C, D26A mutant
0.015
isopentenyl diphosphate
mutant enzyme N77D, pH 7.5, 37°C
0.0157
isopentenyl diphosphate
mutant enzyme R77A, pH 7.5, 25°C
0.0157
isopentenyl diphosphate
-
mutant enzyme R77A, pH 7.5, 25°C
0.0157
isopentenyl diphosphate
pH 7.5, 25°C, R77A mutant
0.016
isopentenyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H199A
0.016
isopentenyl diphosphate
-
pH 7.5, 25°C, H199A mutant
0.0162
isopentenyl diphosphate
-
-
0.0162
isopentenyl diphosphate
mutant enzyme E73A, pH 7.5, 25°C
0.0162
isopentenyl diphosphate
-
pH 7.5, 25°C, wild type
0.0162
isopentenyl diphosphate
pH 7.5, 25°C, E73A mutant
0.0165
isopentenyl diphosphate
mutant enzyme W78R, pH 7.5, 37°C
0.017
isopentenyl diphosphate
mutant enzyme D4226A, pH 7.3, 37°C
0.0175
isopentenyl diphosphate
pH 7.5, 25°C, wild type with 3 mM MgCl2
0.0175
isopentenyl diphosphate
pH 7.5, 30°C, W210A mutant
0.0177
isopentenyl diphosphate
mutant enzyme D221A, pH 7.3, 37°C
0.018
isopentenyl diphosphate
-
pH 7.5, 25°C, mutant F89A, with geranylgeranyl diphosphate
0.018
isopentenyl diphosphate
pH 7.5, 30°C, recombinant mutant W210A
0.018
isopentenyl diphosphate
-
F89A mutant, with geranylgeranyl diphosphate, 25°C, pH 7.5
0.0183
isopentenyl diphosphate
mutant enzyme E201Q, pH 7.3, 37°C
0.02
isopentenyl diphosphate
-
mutant enzyme W149F, pH 7.5, 25°C
0.0204
isopentenyl diphosphate
mutant enzyme E193Q, pH 7.3, 37°C
0.0208
isopentenyl diphosphate
pH 7.5, 25°C, D26E mutant with 0.5 mM MgCl2
0.0223
isopentenyl diphosphate
pH 7.5, 25°C, D26R mutant with 0.5 mM MgCl2
0.0229
isopentenyl diphosphate
pH 7.5, 30°C, Y148F mutant
0.023
isopentenyl diphosphate
-
mutant enzyme W207F, pH 7.5, 25°C
0.023
isopentenyl diphosphate
pH 7.5, 30°C, recombinant mutant Y148F
0.0242
isopentenyl diphosphate
mutant enzyme R42G, pH 7.5, 37°C
0.0242
isopentenyl diphosphate
R42G mutant, pH 7.5, 37°C
0.0254
isopentenyl diphosphate
pH 7.5, 30°C, Y71S mutant
0.026
isopentenyl diphosphate
-
mutant enzyme W75F, pH 7.5, 25°C
0.026
isopentenyl diphosphate
pH 7.5, 30°C, recombinant mutant Y71S
0.027
isopentenyl diphosphate
-
-
0.029
isopentenyl diphosphate
-
pH 7.5, 30°C
0.0307
isopentenyl diphosphate
mutant enzyme E84Q, pH 7.5, 37°C
0.0307
isopentenyl diphosphate
E84Q mutant, pH 7.5, 37°C
0.034
isopentenyl diphosphate
-
pH 7.5, 25°C, S83(Ala)1 mutant
0.0356
isopentenyl diphosphate
pH 7.5, 30°C, W224A mutant
0.036
isopentenyl diphosphate
pH 7.5, 30°C, recombinant mutant Y148F
0.042
isopentenyl diphosphate
-
pH 7.5, 25°C, mutant L88A, with geranylgeranyl diphosphate
0.042
isopentenyl diphosphate
-
L88A mutant, with geranylgeranyl diphosphate, 25°C, pH 7.5
0.0452
isopentenyl diphosphate
mutant enzyme D29A, pH 7.5, 37°C
0.0452
isopentenyl diphosphate
D29A mutant, pH 7.5, 37°C
0.046
isopentenyl diphosphate
mutant enzyme W75A, pH 7.5, 25°C
0.046
isopentenyl diphosphate
pH 7.5, 25°C, W75A mutant
0.0491
isopentenyl diphosphate
mutant enzyme R80A, pH 7.5, 37°C
0.0491
isopentenyl diphosphate
R80A mutant, pH 7.5, 37°C
0.0561
isopentenyl diphosphate
mutant enzyme R197S, pH 7.3, 37°C
0.06
isopentenyl diphosphate
-
pH 7.5, 25°C, mutant L85A, with geranylgeranyl diphosphate
0.06
isopentenyl diphosphate
-
L85A mutant, with geranylgeranyl diphosphate, 25°C, pH 7.5
0.0621
isopentenyl diphosphate
mutant enzyme R203S, pH 7.3, 37°C
0.063
isopentenyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H43A
0.063
isopentenyl diphosphate
-
pH 7.5, 25°C, H43A mutant
0.084
isopentenyl diphosphate
-
pH 7.5, 25°C, mutant F89A, with farnesyl diphosphate
0.084
isopentenyl diphosphate
-
F89A mutant, with (2E,6E)-farnesyl diphosphate, 25°C, pH 7.5
0.088
isopentenyl diphosphate
mutant enzyme E81A, pH 7.5, 25°C
0.088
isopentenyl diphosphate
pH 7.5, 25°C, E81A mutant
0.0909
isopentenyl diphosphate
mutant enzyme E216Q, pH 7.3, 37°C
0.103
isopentenyl diphosphate
pH 7.5, 30°C, recombinant mutant F207S
0.1031
isopentenyl diphosphate
pH 7.5, 30°C, F207S mutant
0.1268
isopentenyl diphosphate
mutant enzyme S74A, pH 7.3, 37°C
0.133
isopentenyl diphosphate
mutant enzyme S71A, pH 7.5, 25°C
0.133
isopentenyl diphosphate
pH 7.5, 25°C, S71A mutant
0.173
isopentenyl diphosphate
-
pH 7.5, 25°C, mutant L88A, with farnesyl diphosphate
0.173
isopentenyl diphosphate
-
L88A mutant, with (2E,6E)-farnesyl diphosphate, 25°C, pH 7.5
0.2525
isopentenyl diphosphate
mutant enzyme F73A, pH 7.3, 37°C
0.27
isopentenyl diphosphate
-
mutant enzyme D150A, pH 7.5, 25°C
0.279
isopentenyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H199A/E213A
0.279
isopentenyl diphosphate
-
pH 7.5, 25°C, H199A/E213A mutant
0.28
isopentenyl diphosphate
-
mutant enzyme D213A, pH 7.5, 25°C
0.28
isopentenyl diphosphate
-
pH 7.5, 25°C, recombinant mutant E213A
0.28
isopentenyl diphosphate
-
pH 7.5, 25°C, E213A mutant
0.291
isopentenyl diphosphate
pH 7.5, 25°C, wild type with 50 mM MgCl2
0.617
isopentenyl diphosphate
-
pH 7.5, 25°C, mutant L85A, with farnesyl diphosphate
0.617
isopentenyl diphosphate
-
L85A mutant, with (2E,6E)-farnesyl diphosphate, 25°C, pH 7.5
7.8
isopentenyl diphosphate
wild type, pH 7.3, 37°C
17
isopentenyl diphosphate
D226A mutant, pH 7.3, 37°C
17.7
isopentenyl diphosphate
D221A mutant, pH 7.3, 37°C
18.3
isopentenyl diphosphate
E201Q mutant, pH 7.3, 37°C
20.4
isopentenyl diphosphate
E193Q mutant, pH 7.3, 37°C
56.1
isopentenyl diphosphate
R197S mutant, pH 7.3, 37°C
62.1
isopentenyl diphosphate
R203S mutant, pH 7.3, 37°C
90.9
isopentenyl diphosphate
E216Q mutant, pH 7.3, 37°C
126.8
isopentenyl diphosphate
S74A mutant, pH 7.3, 37°C
252.5
isopentenyl diphosphate
F73A mutant, pH 7.3, 37°C
0.0267
neryl diphosphate
-
pH 8.5, 37°C
0.032
neryl diphosphate
-
pH 7.5, 35°C
additional information
additional information
-
steady-state kinetics
-
additional information
additional information
-
steady-state kinetics, single turnover measurements with substrate geranylgeranyl diphosphate
-
additional information
additional information
-
fluorescence-based UPPS kinetic measurements, kinetics of the single-turnover reaction of (2E,6E)-8-O-(N-methyl-2-aminobenzoyl)-3,7-dimethyl-2,6-octandien-1-diphosphate chain elongation catalyzed by UPPS, overview
-
additional information
additional information
-
pre-steady-state UPPS kinetics, single-turnover, kinetic analysis, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
11.2
(2E,6E)-8-(N-methyl-2-aminobenzoyloxy)-3,7-dimethyl-2,6-octandien-1-diphosphate
-
pH 7.5, 25°C
0.0000633 - 3.5
(2E,6E)-farnesyl diphosphate
0.00000031
(2E,6E)-farnesyl thiodiphosphate
-
much smaller than using (2E,6E)-farnesyl diphosphate, pH 7.5, 25°C
0.002 - 1.77
(2Z,6E,10E)-geranylgeranyl diphosphate
0.002 - 1.77
(Z,E,E)-Geranylgeranyl diphosphate
0.02
7-(2,6-dimethyl-8-diphospho-2,6-octadienyloxy)-8-methyl-4-trifluoromethyl-chromen-2-one geranyl diphosphate
-
alternative substrate, pH 7.5, 25°C
0.00006 - 2.5
farnesyl diphosphate
1.7
geranyl diphosphate
-
25°C, pH 7.5
0.18 - 2.1
geranylgeranyl diphosphate
0.0002 - 2.5
isopentenyl diphosphate
additional information
additional information
-
0.0000633
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, D26R mutant with 0.5 mM MgCl2
0.0000766
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, D26K mutant with 0.5 mM MgCl2
0.00013
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, S83(Ala)5 mutant
0.00014
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, R77A mutant
0.00022
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, DELTAV82S83 mutant
0.00028
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, DELTAS72 mutant
0.000366
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, D26E mutant with 0.5 mM MgCl2
0.00076
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, DELTAS83 mutant
0.0009
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, S83(Ala)1 mutant
0.00125
(2E,6E)-farnesyl diphosphate
mutant enzyme N77A, pH 7.5, 37°C
0.00155
(2E,6E)-farnesyl diphosphate
mutant enzyme N77Q, pH 7.5, 37°C
0.0025
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H199A/E213A
0.0026
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H43A and mutant E213A
0.00272
(2E,6E)-farnesyl diphosphate
mutant enzyme N77D, pH 7.5, 37°C
0.0033
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant D26A
0.0033
(2E,6E)-farnesyl diphosphate
-
mutant enzyme D26A, pH 7.5, 25°C
0.004
(2E,6E)-farnesyl diphosphate
R80A mutant, pH 7.5, 37°C
0.013
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, without 0.1% Triton
0.0167
(2E,6E)-farnesyl diphosphate
D29A mutant, pH 7.5, 37°C
0.022
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, N74A mutant
0.026
(2E,6E)-farnesyl diphosphate
-
mutant enzyme E213A, pH 7.5, 25°C
0.0542
(2E,6E)-farnesyl diphosphate
R33A mutant, pH 7.5, 37°C
0.095
(2E,6E)-farnesyl diphosphate
-
L85A mutant, 25°C, pH 7.5
0.11
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, S71A mutant
0.151
(2E,6E)-farnesyl diphosphate
mutant enzyme W78D, pH 7.5, 37°C
0.2
(2E,6E)-farnesyl diphosphate
mutant C234A, co-substrate: isopentenyl diphosphate
0.2
(2E,6E)-farnesyl diphosphate
mutant C234A, pH 7.5, temperature not specified in the publication
0.216
(2E,6E)-farnesyl diphosphate
R42G mutant, pH 7.5, 37°C
0.22
(2E,6E)-farnesyl diphosphate
wild-type, co-substrate: isopentenyl diphosphate
0.22
(2E,6E)-farnesyl diphosphate
-
mutant enzyme D218A, pH 7.5, 25°C
0.22
(2E,6E)-farnesyl diphosphate
wild type, pH 7.5, temperature not specified in the publication
0.23
(2E,6E)-farnesyl diphosphate
-
L88A mutant, 25°C, pH 7.5
0.3
(2E,6E)-farnesyl diphosphate
-
mutant enzymes E73A, pH 7.5, 25°C
0.3
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, E73A mutant
0.4
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, E81A mutant
0.434
(2E,6E)-farnesyl diphosphate
mutant enzyme N78R, pH 7.5, 37°C
0.456
(2E,6E)-farnesyl diphosphate
mutant enzyme W78I, pH 7.5, 37°C
0.462
(2E,6E)-farnesyl diphosphate
G32R mutant, pH 7.5, 37°C
0.505
(2E,6E)-farnesyl diphosphate
E76Q mutant, pH 7.5, 37°C
0.61
(2E,6E)-farnesyl diphosphate
-
F89A mutant, 25°C, pH 7.5
0.96
(2E,6E)-farnesyl diphosphate
G32R/R42G mutant, pH 7.5, 37°C
1.09
(2E,6E)-farnesyl diphosphate
E84Q mutant, pH 7.5, 37°C
1.1
(2E,6E)-farnesyl diphosphate
-
mutant enzyme D150A, pH 7.5, 25°C
1.1
(2E,6E)-farnesyl diphosphate
-
mutant enzyme W31F, pH 7.5, 25°C
1.1
(2E,6E)-farnesyl diphosphate
-
mutant enzyme W75F, pH 7.5, 25°C
1.1
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, W75A mutant
1.22
(2E,6E)-farnesyl diphosphate
wild-type enzyme, pH 7.5, 37°C
1.23
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, wild type with 0.05 mM MgCl2
1.5
(2E,6E)-farnesyl diphosphate
-
mutant enzyme W207F, pH 7.5, 25°C
1.66
(2E,6E)-farnesyl diphosphate
wild-type, pH 7.5, 37°C
1.7
(2E,6E)-farnesyl diphosphate
-
mutant enzyme W149F, pH 7.5, 25°C
1.92
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, wild type with 3 mM MgCl2
1.98
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, wild type with 1 mM MgCl2
2.2
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H199A
2.5
(2E,6E)-farnesyl diphosphate
-
wild-type enzyme, pH 7.5, 25°C
2.5
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C
2.5
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, wild type
2.5
(2E,6E)-farnesyl diphosphate
pH 7.5, 25°C, wild type
2.5
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, recombinant wild-type enzyme
2.5
(2E,6E)-farnesyl diphosphate
-
mutant enzyme W91F, pH 7.5, 25°C
2.5
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 25°C, in the presence of 0.1% Triton
2.5
(2E,6E)-farnesyl diphosphate
-
wild type, 25°C, pH 7.5
2.5
(2E,6E)-farnesyl diphosphate
-
wild type, pH 7.5, 25°C
3.5
(2E,6E)-farnesyl diphosphate
-
mutant enzyme W221F, pH 7.5, 25°C
0.002
(2Z,6E,10E)-geranylgeranyl diphosphate
mutant enzyme N77A, pH 7.5, 37°C
0.00231
(2Z,6E,10E)-geranylgeranyl diphosphate
mutant enzyme N77Q, pH 7.5, 37°C
0.00415
(2Z,6E,10E)-geranylgeranyl diphosphate
mutant enzyme N77D, pH 7.5, 37°C
0.00579
(2Z,6E,10E)-geranylgeranyl diphosphate
R80A mutant, pH 7.5, 37°C
0.0258
(2Z,6E,10E)-geranylgeranyl diphosphate
D29A mutant, pH 7.5, 37°C
0.0813
(2Z,6E,10E)-geranylgeranyl diphosphate
R33A mutant, pH 7.5, 37°C
0.3
(2Z,6E,10E)-geranylgeranyl diphosphate
mutant enzyme W78D, pH 7.5, 37°C
0.382
(2Z,6E,10E)-geranylgeranyl diphosphate
R42G mutant, pH 7.5, 37°C
0.604
(2Z,6E,10E)-geranylgeranyl diphosphate
E76Q mutant, pH 7.5, 37°C
0.608
(2Z,6E,10E)-geranylgeranyl diphosphate
G32R mutant, pH 7.5, 37°C
0.899
(2Z,6E,10E)-geranylgeranyl diphosphate
mutant enzyme W78R, pH 7.5, 37°C
1.07
(2Z,6E,10E)-geranylgeranyl diphosphate
E84Q mutant, pH 7.5, 37°C
1.11
(2Z,6E,10E)-geranylgeranyl diphosphate
G32R/R42G mutant, pH 7.5, 37°C
1.37
(2Z,6E,10E)-geranylgeranyl diphosphate
mutant enzyme W78I, pH 7.5, 37°C
1.77
(2Z,6E,10E)-geranylgeranyl diphosphate
wild-type enzyme, pH 7.5, 37°C
1.77
(2Z,6E,10E)-geranylgeranyl diphosphate
wild-type, pH 7.5, 37°C
0.002
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme N77A, pH 7.5, 37°C
0.00231
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme N77Q, pH 7.5, 37°C
0.00415
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme N77D, pH 7.5, 37°C
0.3
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme W78D, pH 7.5, 37°C
0.899
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme W78R, pH 7.5, 37°C
1.37
(Z,E,E)-Geranylgeranyl diphosphate
mutant enzyme W78I, pH 7.5, 37°C
1.77
(Z,E,E)-Geranylgeranyl diphosphate
wild-type enzyme, pH 7.5, 37°C
0.00006
farnesyl diphosphate
pH 7.5, 25°C, mutant D26R
0.00008
farnesyl diphosphate
pH 7.5, 25°C, mutant D26K
0.0004
farnesyl diphosphate
pH 7.5, 25°C, mutant D26E
0.00125
farnesyl diphosphate
mutant enzyme N77A, pH 7.5, 37°C
0.00155
farnesyl diphosphate
mutant enzyme N77Q, pH 7.5, 37°C
0.002
farnesyl diphosphate
pH 7.5, 30°C, recombinant mutant W210A
0.0025
farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H199A/E213A
0.0026
farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H43A and mutant E213A
0.00272
farnesyl diphosphate
mutant enzyme N77D, pH 7.5, 37°C
0.003
farnesyl diphosphate
pH 7.5, 30°C, recombinant mutant F207S
0.0033
farnesyl diphosphate
pH 7.5, 25°C, mutant D26A
0.0033
farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant D26A
0.015
farnesyl diphosphate
pH 7.5, 30°C, recombinant mutant Y148F
0.0167
farnesyl diphosphate
mutant enzyme D29A, pH 7.5, 37°C
0.0542
farnesyl diphosphate
mutant enzyme R33A, pH 7.5, 37°C
0.071
farnesyl diphosphate
pH 7.5, 30°C, recombinant mutant Y148F
0.095
farnesyl diphosphate
-
pH 7.5, 25°C, mutant L85A
0.11
farnesyl diphosphate
pH 7.5, 30°C, recombinant mutant Y71S
0.151
farnesyl diphosphate
mutant enzyme W78D, pH 7.5, 37°C
0.2
farnesyl diphosphate
mutant C234A, pH 7.5
0.216
farnesyl diphosphate
mutant enzyme R42G, pH 7.5, 37°C
0.22
farnesyl diphosphate
wild-type, pH 7.5
0.23
farnesyl diphosphate
-
pH 7.5, 25°C, mutant L88A
0.434
farnesyl diphosphate
mutant enzyme N78R, pH 7.5, 37°C
0.456
farnesyl diphosphate
mutant enzyme W78I, pH 7.5, 37°C
0.462
farnesyl diphosphate
mutant enzyme G32R, pH 7.5, 37°C
0.505
farnesyl diphosphate
mutant enzyme E76Q, pH 7.5, 37°C
0.61
farnesyl diphosphate
-
pH 7.5, 25°C, mutant F89A
0.96
farnesyl diphosphate
mutant enzyme G32R/R42G, pH 7.5, 37°C
1.09
farnesyl diphosphate
mutant enzyme E84Q, pH 7.5, 37°C
1.22
farnesyl diphosphate
wild-type enzyme, pH 7.5, 37°C
1.22
farnesyl diphosphate
pH 7.5, 30°C, recombinant wild-type enzyme
1.66
farnesyl diphosphate
wild-type enzyme, pH 7.5, 37°C
2.2
farnesyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H199A
2.5
farnesyl diphosphate
-
pH 7.5, 25°C, wild-type enzyme
2.5
farnesyl diphosphate
pH 7.5, 25°C, wild-type enzyme
2.5
farnesyl diphosphate
-
pH 7.5, 25°C, recombinant wild-type enzyme
0.18
geranylgeranyl diphosphate
-
pH 7.5, 25°C, mutant L85A
0.18
geranylgeranyl diphosphate
-
L85A mutant, 25°C, pH 7.5
0.32
geranylgeranyl diphosphate
-
pH 7.5, 25°C, mutant L88A
0.32
geranylgeranyl diphosphate
-
L88A mutant, 25°C, pH 7.5
0.7
geranylgeranyl diphosphate
-
pH 7.5, 25°C, mutant F89A
0.7
geranylgeranyl diphosphate
-
F89A mutant, 25°C, pH 7.5
2.1
geranylgeranyl diphosphate
-
pH 7.5, 25°C, wild-type enzyme
2.1
geranylgeranyl diphosphate
-
wild type, 25°C, pH 7.5
0.0002
isopentenyl diphosphate
-
-
0.002
isopentenyl diphosphate
pH 7.5, 30°C, recombinant mutant W210A
0.0025
isopentenyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H199A/E213A
0.0026
isopentenyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H43A and mutant E213A
0.0027
isopentenyl diphosphate
mutant enzyme N77Q, pH 7.5, 37°C
0.0033
isopentenyl diphosphate
-
pH 7.5, 25°C, recombinant mutant D26A
0.00473
isopentenyl diphosphate
mutant enzyme N77D, pH 7.5, 37°C
0.01
isopentenyl diphosphate
pH 7.5, 30°C, recombinant mutant F207S
0.0164
isopentenyl diphosphate
mutant enzyme N77A, pH 7.5, 37°C
0.071
isopentenyl diphosphate
pH 7.5, 30°C, recombinant mutant Y148F
0.11
isopentenyl diphosphate
pH 7.5, 30°C, recombinant mutant Y71S
0.115
isopentenyl diphosphate
pH 7.5, 30°C, recombinant mutant Y148F
0.2
isopentenyl diphosphate
mutant C234A, co-substrate: farnesyl diphosphate
0.22
isopentenyl diphosphate
wild-type, co-substrate: farnesyl diphosphate
0.397
isopentenyl diphosphate
mutant enzyme W78D, pH 7.5, 37°C
0.783
isopentenyl diphosphate
mutant enzyme W78I, pH 7.5, 37°C
0.803
isopentenyl diphosphate
mutant enzyme W78R, pH 7.5, 37°C
1.55
isopentenyl diphosphate
pH 7.5, 30°C, recombinant wild-type enzyme
1.85
isopentenyl diphosphate
wild-type, pH 7.5, 37°C
2.2
isopentenyl diphosphate
-
pH 7.5, 25°C, recombinant mutant H199A
2.5
isopentenyl diphosphate
-
pH 7.5, 25°C, recombinant wild-type enzyme
additional information
additional information
-
-
-
additional information
additional information
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
additional information
additional information
-
turnover number for recombinant wild-type enzyme and mutant enzymes Y71S, Y148F, F207S, W210A, W224A
-
additional information
additional information
turnover number for recombinant wild-type enzyme and mutant enzymes Y71S, Y148F, F207S, W210A, W224A
-
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0.2
(1-hydroxy-2-phenylethane-1,1-diyl)bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0011
(1-hydroxy-2-[3'-[(naphthalene-2-sulfonyl)amino][1,1'-biphenyl]-3-yl]ethane-1,1-diyl)bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0056 - 0.014
(2S,3R,4S,5S,6R)-2-(3-((4-((3-(4,5-dihydro-1H-imidazol-2-yl)-phenyl)ethynyl)phenyl)ethynyl)phenoxy)-6-(hydroxymethyl)-tetrahydro-2H-pyran-3,4,5-triol
0.0022
(2Z)-2-hydroxy-4-oxo-4-[[3-(3-phenoxyphenyl)propyl]amino]but-2-enoic acid
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0005
(2Z)-2-hydroxy-4-[3-[3-(octyloxy)phenoxy]phenyl]-4-oxobut-2-enoic acid
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00051
(2Z)-2-hydroxy-4-[4-(octyloxy)phenyl]-4-oxobut-2-enoic acid
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00056
(2Z)-4-([3-[3-(hexyloxy)phenyl]propyl]amino)-2-hydroxy-4-oxobut-2-enoic acid
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0019
(2Z)-4-[3-[3-(decyloxy)phenoxy]phenyl]-2-hydroxy-4-oxobut-2-enoic acid
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00024
(2Z)-4-[[3-([1,1'-biphenyl]-3-yl)propyl]amino]-2-hydroxy-4-oxobut-2-enoic acid
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0025
(5-bromo-2-[[3-(octyloxy)phenyl]methoxy]phenyl)phosphonic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00047 - 0.00068
1,4-bis(6-(1,4,5,6-tetrahydropyrimidin-2-yl)-1H-indol-2-yl)-benzene
0.00036 - 0.0017
1,4-bis(6-(4,5-dihydro-1H-imidazol-2-yl)-1H-indol-2-yl)benzene
0.048
1-(2-hydroxy-2,2-diphosphonoethyl)-3-phenylpyridin-1-ium
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0429
1-[3-fluoro-4-(4-[[2-(thiophen-2-yl)-1,3-thiazol-4-yl]methyl]piperazin-1-yl)phenyl]ethan-1-one
Bacillus subtilis
pH and temperature not specified in the publication, mutant enzyme Y246D
0.027
1-[[3-(hexyloxy)phenyl]methyl]-4-hydroxy-2-oxo-1,2-dihydropyridine-3-carboxylic acid
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0014 - 0.0061
2,2'-(5,5'-(1,3-phenylenebis(ethyne-2,1-diyl))bis(3-bromo-5,1-phenylene))diethanamine
0.0051 - 0.012
2,2'-(E)-ethene-1,2-diylbis[6-(4,5-dihydro-1H-imidazol-2-yl)-1H-indole]
0.00139 - 0.042
2,6-bis(5-bromo-2-hydroxyphenyl)-3,5-dimethylpiperidin-4-one
0.024
2-(2-[[5-(1-benzofuran-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl]sulfanyl]acetamido)benzoic acid
Escherichia coli
pH 7.5, 25°C
0.0022
2-hydroxy-6-(undecyloxy)benzoic acid
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.006
2-methoxy-6-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0049
2-[3-(decyloxy)benzamido]-5-nitrobenzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0024
2-[3-(hexyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00078
2-[3-(octyloxy)benzamido]-5-(trifluoromethoxy)benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.003
2-[3-(octyloxy)benzamido]-5-phosphonobenzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0016
2-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.008
2-[methyl[3-(octyloxy)benzoyl]amino]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.045
2-[[(2E)-2-cyano-3-[1-[(naphthalen-1-yl)methyl]-1H-indol-3-yl]prop-2-enoyl]amino]benzoic acid
Escherichia coli
pH 7.5, 25°C
0.025
2-[[(2E)-2-cyano-3-[3-[(4-fluorophenyl)methoxy]phenyl]prop-2-enoyl]amino]benzoic acid
Escherichia coli
pH 7.5, 25°C
0.15
2-[[3-(3,4-dimethylphenoxy)phenyl]carbamoyl]benzoic acid
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00005
3-(2-chlorophenyl)-N-(4-isopropylphenyl-5-methylisoxazole-4-carboxamide)
Streptococcus pneumoniae
pH 7.5, 37°C
0.034
3-(dibenzo[b,d]furan-4-yl)-1-(2,2-diphosphonoethyl)pyridin-1-ium
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.12
3-([1,1'-biphenyl]-2-yl)-1-(2,2-diphosphonoethyl)pyridin-1-ium
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.068
3-([1,1'-biphenyl]-3-yl)-1-(2,2-diphosphonoethyl)pyridin-1-ium
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.01
3-([1,1'-biphenyl]-3-yl)-1-(2-hydroxy-2,2-diphosphonoethyl)pyridin-1-ium
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.035
3-([1,1'-biphenyl]-4-yl)-1-(2,2-diphosphonoethyl)pyridin-1-ium
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.01166
3-ethoxy-2-methyl-6-(4-phenyl-1H-pyrazol-3-yl)phenol
Bacillus subtilis
pH and temperature not specified in the publication, mutant enzyme Y246D
0.49
3-fluoro-1-(2-hydroxy-2,2-diphosphonoethyl)pyridin-1-ium
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.089
3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-1-(2,2-diphosphonoethyl)pyridin-1-ium
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0049
3-[3-(hexyloxy)benzamido]-4-hydroxybenzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0045
3-[3-(octyloxy)benzamido]benzene-1,2-dicarboxylic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.071 - 0.362
3-[5-(5,6-dihydrobenzimidazo[1,2-c]quinazolin-6-yl)-2,5-dihydrofuran-2-yl]benzenesulfonamide
0.24 - 0.4
4,4'-[pentane-1,5-diylbis(oxy)]dibenzenecarboximidamide
0.00096
4,5-difluoro-2-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0013 - 0.038
4-(benzyloxy)-3-bromo-5-methoxy-N-[(oxolan-2-yl)methyl]benzamide
0.013
4-(dibenzo[b,d]furan-4-yl)-1-(2-hydroxy-2,2-diphosphonoethyl)pyridin-1-ium
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.021
4-([1,1'-biphenyl]-3-yl)-1-(2,2-diphosphonoethyl)pyridin-1-ium
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.028
4-([1,1'-biphenyl]-4-yl)-1-(2,2-diphosphonoethyl)pyridin-1-ium
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0085
4-[3-(octyloxy)benzamido]benzene-1,3-dicarboxylic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0032
4-[3-[([1,1'-biphenyl]-4-carbonyl)amino]phenoxy]benzene-1,2-dicarboxylic acid
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0032 - 0.0034
5-(diethoxyphosphoryl)-2-[3-(octyloxy)benzamido]benzoic acid
0.002
5-(methanesulfonyl)-2-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00004
5-benzyl-3-[1-(4-cyclohexylanilino)ethenyl]-4-hydroxy-5,6-dihydropyridin-2(1H)-one
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00063
5-benzyl-4-hydroxy-3-[1-[4-(1H-imidazol-1-yl)anilino]ethenyl]-5,6-dihydropyridin-2(1H)-one
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00063
5-benzyl-4-hydroxy-5-methyl-2-oxo-2,5-dihydro-1H-pyrrole-3-carboxylic acid (4-cyclohexylphenyl)amide
Streptococcus pneumoniae
-
pH 7.3, temperature not specified in the publication, N-(4-benzoylphenyl)-4-hydroxy-2-oxo-5-phenethyl-2,5-dihydro-1H-pyrrole-3-carboxamide as substrate
0.0005
5-benzyl-N-(4-cyclohexylphenyl)-4-hydroxy-2-oxo-2,5-dihydro-1H-pyrrole-3-carboxamide
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.019
5-benzyl-N-[4-(hexyloxy)phenyl]-4-hydroxy-2-oxo-2,5-dihydro-1H-pyrrole-3-carboxamide
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0069
5-bromo-2-[3-(3,3-dimethylbut-1-yn-1-yl)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00054
5-bromo-2-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.001
5-bromo-2-[4-(6-chloro-2,3-dihydro-1H-indole-1-sulfonyl)benzamido]benzoic acid
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0006
5-chloro-2-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0049
5-fluoro-2-[3-(hexyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00032 - 0.00033
5-fluoro-2-[3-(octyloxy)benzamido]benzoic acid
0.0013
5-hydroxy-2-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0013
5-methoxy-2-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.01
5-methoxy-4-(2-methoxyethoxy)-2-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.007
5-[3-(octyloxy)benzamido]benzene-1,3-dicarboxylic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0088
6-benzyl-4-hydroxy-3-[1-[4-(1H-imidazol-1-yl)anilino]ethenyl]-5,6-dihydropyridin-2(1H)-one
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00005 - 0.0016
7-(azepan-1-yl)-3-(4-fluorophenyl)-5-methylpyrazolo[1,5-a]pyrimidine
0.0006
BPH-629
Escherichia coli
-
pH and temperature not specified in the publication
0.0013 - 0.0039
farnesyl thiodiphosphate
0.01166
MAC-0110792
Escherichia coli
pH and temperature not specified in the publication
0.00005 - 0.0016
MAC-0547630
0.00139 - 0.0397
MAC-0557874
0.0013 - 0.038
MAC-0588238
0.0429
MAC-0602693
Bacillus subtilis
pH and temperature not specified in the publication
0.071 - 0.35
N,N'-bis(6-chloro-1,3-benzothiazol-2-yl)methanedisulfonamide
0.06
N-(2-carbamoylphenyl)-3-(octyloxy)benzamide
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.002
N-(3,5-dichlorophenyl)-4-hydroxy-2-oxo-5-phenyl-2,5-dihydro-1H-pyrrole-3-carboxamide
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00097
N-(3-amino-3-oxopropyl)-1-benzyl-5-(4-methylphenyl)-N-([4-[(propan-2-yl)oxy]phenyl]methyl)-1H-pyrazole-4-carboxamide
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00019
N-(3-amino-3-oxopropyl)-5-(1-benzothiophen-5-yl)-1-benzyl-N-([4-[(propan-2-yl)oxy]phenyl]methyl)-1H-pyrazole-4-carboxamide
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.13
N-(4-bromo-2-carbamoylphenyl)-3,4-bis(2-methoxyethoxy)benzamide
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.024
N-(4-chlorophenyl)-4-hydroxy-2-oxo-5-phenyl-2,5-dihydro-1H-pyrrole-3-carboxamide
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.058
N-(4-cyclohexylphenyl)-4-hydroxy-2-oxo-2,5-dihydro-1H-pyrrole-3-carboxamide
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00016
N-(4-cyclohexylphenyl)-4-hydroxy-2-oxo-5-(2-phenylethyl)-2,5-dihydro-1H-pyrrole-3-carboxamide
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0035
N-(4-cyclohexylphenyl)-4-hydroxy-2-oxo-5-phenyl-2,5-dihydro-1H-pyrrole-3-carboxamide
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00012
N-([1,1'-biphenyl]-4-yl)-4-hydroxy-2-oxo-5-(2-phenylethyl)-2,5-dihydro-1H-pyrrole-3-carboxamide
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
1 - 1.4
N-[2-[(2-[1-[(3-amino-3-iminopropyl)amino]ethenyl]-1-methyl-1H-imidazol-4-yl)acetyl]-1-methyl-1H-imidazol-4-yl]-N2-(diaminomethylidene)glycinamide
0.0057 - 0.011
N-[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-N'-(3-phenoxyphenyl)biphenyl-4,4'-dicarboxamide
0.0032
N-[3-[3,4-di(prop-1-en-2-yl)phenoxy]phenyl][1,1'-biphenyl]-4-carboxamide
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0048
N1,N4-bis(4-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-2-nitroterephthalamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.0049
N1,N4-bis(4-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-2-nitroterephthalamide dihydrochloride
Staphylococcus aureus
-
pH 7.5, 37°C
0.0048
N1,N4-bis[4-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-2-nitrobenzene-1,4-dicarboxamide
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0045 - 0.0055
N4,N4'-bis(3-(1,4,5,6-tetrahydropyrimidin-2-yl)phenyl)biphenyl-4,4'-dicarboxamide
0.00011
N4,N4'-bis(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-[1,1'-biphenyl]-4,4'-bicarboxamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.0035 - 0.0092
N4,N4'-bis(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)biphenyl-4,4'-bis(carbothioamide)
0.38 - 0.53
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'- (3-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)phenyl)-[1,1-biphenyl]-4,4-dicarboxamide
0.0089 - 0.025
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-(3-(hexyloxy)-phenyl)-[1,1-biphenyl]-4,4-dicarboxamide
0.0021 - 0.0094
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-(3-methoxyphenyl)-[1,1-biphenyl]-4,4-dicarboxamide
0.0003
tetramic acid
Streptococcus pneumoniae
pH and temperature not specified in the publication
0.00036 - 0.00039
[1-hydroxy-2-(3-[3-[3-(2-hydroxy-2,2-bis-phosphono-ethyl)-biphenyl-3-ylsulfamoyl]-benzenesulfonylamino]-biphenyl-3-yl)-1-phosphono-ethyl]-phosphonic acid
0.66
[1-hydroxy-2-(pyridin-3-yl)ethane-1,1-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00069
[1-hydroxy-2-([1(1),2(1):2(3),3(1)-terphenyl]-1(3)-yl)ethane-1,1-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.002
[1-hydroxy-2-([1(1),2(1):2(3),3(1)-terphenyl]-1(4)-yl)ethane-1,1-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
-
0.00091
[1-hydroxy-2-([1(1),2(1):2(4),3(1)-terphenyl]-1(3)-yl)ethane-1,1-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0013
[1-hydroxy-2-[([1(1),2(1):2(3),3(1)-terphenyl]-1(3)-yl)oxy]ethane-1,1-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0031
[1-hydroxy-3-([1(1),2(1):2(2),3(1)-terphenyl]-1(3)-yl)propane-1,1-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0055
[1-hydroxy-3-([1(1),2(1):2(3),3(1)-terphenyl]-1(3)-yl)propane-1,1-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.01
[1-hydroxy-3-([1(1),2(1):2(4),3(1)-terphenyl]-1(3)-yl)propane-1,1-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0068
[2-([1,1'-biphenyl]-3-yl)-1-hydroxyethane-1,1-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00059
[2-[3-(dibenzo[b,d]furan-4-yl)phenyl]-1-hydroxy-1,1-ethanediyl]bis(phosphonic acid)
Escherichia coli
pH 7.5, 25°C
0.00059
[2-[3-(dibenzo[b,d]furan-4-yl)phenyl]-1-hydroxyethane-1,1-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.039
[2-[3-(octyloxy)benzamido]phenyl]phosphonic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.087
[3-(3,4-dichlorophenyl)-4,5-dihydro-1,2-oxazole-5,5-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.012
[3-[4-(dibenzo[b,d]furan-4-yl)phenyl]-1-hydroxypropane-1,1-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.069
[4-([1,1'-biphenyl]-4-yl)butane-1,1-diyl]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0018
[hydroxy([1(1),2(1):2(3),3(1)-terphenyl]-1(3)-yl)methylene]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0078
[[(9-ethyl-9H-carbazol-3-yl)amino](hydroxy)methylene]bis(phosphonic acid)
Escherichia coli
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0056
(2S,3R,4S,5S,6R)-2-(3-((4-((3-(4,5-dihydro-1H-imidazol-2-yl)-phenyl)ethynyl)phenyl)ethynyl)phenoxy)-6-(hydroxymethyl)-tetrahydro-2H-pyran-3,4,5-triol
Staphylococcus aureus
-
pH 7.5, 37°C
0.014
(2S,3R,4S,5S,6R)-2-(3-((4-((3-(4,5-dihydro-1H-imidazol-2-yl)-phenyl)ethynyl)phenyl)ethynyl)phenoxy)-6-(hydroxymethyl)-tetrahydro-2H-pyran-3,4,5-triol
Staphylococcus aureus
-
pH 7.5, 37°C
0.00047
1,4-bis(6-(1,4,5,6-tetrahydropyrimidin-2-yl)-1H-indol-2-yl)-benzene
Staphylococcus aureus
-
pH 7.5, 37°C
0.00068
1,4-bis(6-(1,4,5,6-tetrahydropyrimidin-2-yl)-1H-indol-2-yl)-benzene
Staphylococcus aureus
-
pH 7.5, 37°C
0.00036
1,4-bis(6-(4,5-dihydro-1H-imidazol-2-yl)-1H-indol-2-yl)benzene
Staphylococcus aureus
-
pH 7.5, 37°C
0.0017
1,4-bis(6-(4,5-dihydro-1H-imidazol-2-yl)-1H-indol-2-yl)benzene
Staphylococcus aureus
-
pH 7.5, 37°C
0.0014
2,2'-(5,5'-(1,3-phenylenebis(ethyne-2,1-diyl))bis(3-bromo-5,1-phenylene))diethanamine
Staphylococcus aureus
-
pH 7.5, 37°C
0.0061
2,2'-(5,5'-(1,3-phenylenebis(ethyne-2,1-diyl))bis(3-bromo-5,1-phenylene))diethanamine
Staphylococcus aureus
-
pH 7.5, 37°C
0.0051
2,2'-(E)-ethene-1,2-diylbis[6-(4,5-dihydro-1H-imidazol-2-yl)-1H-indole]
Staphylococcus aureus
-
pH 7.5, 37°C
0.012
2,2'-(E)-ethene-1,2-diylbis[6-(4,5-dihydro-1H-imidazol-2-yl)-1H-indole]
Staphylococcus aureus
-
pH 7.5, 37°C
0.00139
2,6-bis(5-bromo-2-hydroxyphenyl)-3,5-dimethylpiperidin-4-one
Bacillus subtilis
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00622
2,6-bis(5-bromo-2-hydroxyphenyl)-3,5-dimethylpiperidin-4-one
Bacillus subtilis
pH and temperature not specified in the publication, mutant enzyme Y246D
0.042
2,6-bis(5-bromo-2-hydroxyphenyl)-3,5-dimethylpiperidin-4-one
Bacillus subtilis
pH and temperature not specified in the publication, mutant enzyme Y246D
0.071
3-[5-(5,6-dihydrobenzimidazo[1,2-c]quinazolin-6-yl)-2,5-dihydrofuran-2-yl]benzenesulfonamide
Escherichia coli
-
0.362
3-[5-(5,6-dihydrobenzimidazo[1,2-c]quinazolin-6-yl)-2,5-dihydrofuran-2-yl]benzenesulfonamide
Helicobacter pylori
pH 7.5
0.362
3-[5-(5,6-dihydrobenzimidazo[1,2-c]quinazolin-6-yl)-2,5-dihydrofuran-2-yl]benzenesulfonamide
Helicobacter pylori
IC50 value of this compound is equal against the C234A mutant and wild-type
0.362
3-[5-(5,6-dihydrobenzimidazo[1,2-c]quinazolin-6-yl)-2,5-dihydrofuran-2-yl]benzenesulfonamide
Helicobacter pylori
pH 7.5, temperature not specified in the publication
0.24
4,4'-[pentane-1,5-diylbis(oxy)]dibenzenecarboximidamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.4
4,4'-[pentane-1,5-diylbis(oxy)]dibenzenecarboximidamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.0013
4-(benzyloxy)-3-bromo-5-methoxy-N-[(oxolan-2-yl)methyl]benzamide
Bacillus subtilis
pH and temperature not specified in the publication, mutant enzyme Y246D
0.038
4-(benzyloxy)-3-bromo-5-methoxy-N-[(oxolan-2-yl)methyl]benzamide
Bacillus subtilis
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0032
5-(diethoxyphosphoryl)-2-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0034
5-(diethoxyphosphoryl)-2-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00032
5-fluoro-2-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00033
5-fluoro-2-[3-(octyloxy)benzamido]benzoic acid
Staphylococcus aureus
-
pH and temperature not specified in the publication, mutant enzyme Y246D
0.00005
7-(azepan-1-yl)-3-(4-fluorophenyl)-5-methylpyrazolo[1,5-a]pyrimidine
Bacillus subtilis
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0016
7-(azepan-1-yl)-3-(4-fluorophenyl)-5-methylpyrazolo[1,5-a]pyrimidine
Bacillus subtilis
pH and temperature not specified in the publication, mutant enzyme Y246D
0.0075
clomiphene
Staphylococcus aureus
-
pH 7.3, 37°C
0.0075
clomiphene
Staphylococcus aureus
pH 7.3, 37°C
1
diphosphate
Bacteroides fragilis
pH 8.3, 37°C
7.4
diphosphate
Bacteroides fragilis
pH 8.3, 37°C, in presence of 2-nitrileanilinogeranyl diphosphate
0.0013
farnesyl thiodiphosphate
Escherichia coli
-
pH 7.5, 25°C, versus (2E,6E)-farnesyl diphosphate
0.0024 - 0.0039
farnesyl thiodiphosphate
Escherichia coli
-
pH 7.5, 25°C, versus (2E,6E)-8-O-(N-methyl-2-aminobenzoyl)-3,7-dimethyl-2,6-octandien-1-diphosphate
0.00005
MAC-0547630
Bacillus subtilis
pH and temperature not specified in the publication
0.0016
MAC-0547630
Staphylococcus aureus
pH and temperature not specified in the publication
0.00139
MAC-0557874
Escherichia coli
pH and temperature not specified in the publication
0.00622
MAC-0557874
Bacillus subtilis
pH and temperature not specified in the publication
0.0397
MAC-0557874
Staphylococcus aureus
pH and temperature not specified in the publication
0.0013
MAC-0588238
Bacillus subtilis
pH and temperature not specified in the publication
0.038
MAC-0588238
Staphylococcus aureus
pH and temperature not specified in the publication
0.071
N,N'-bis(6-chloro-1,3-benzothiazol-2-yl)methanedisulfonamide
Escherichia coli
-
0.071
N,N'-bis(6-chloro-1,3-benzothiazol-2-yl)methanedisulfonamide
Escherichia coli
pH 7.5
0.071
N,N'-bis(6-chloro-1,3-benzothiazol-2-yl)methanedisulfonamide
Escherichia coli
pH 7.5, temperature not specified in the publication
0.35
N,N'-bis(6-chloro-1,3-benzothiazol-2-yl)methanedisulfonamide
Helicobacter pylori
pH 7.5
0.35
N,N'-bis(6-chloro-1,3-benzothiazol-2-yl)methanedisulfonamide
Helicobacter pylori
IC50 value of this compound is equal against the C234A mutant and wild-type
0.35
N,N'-bis(6-chloro-1,3-benzothiazol-2-yl)methanedisulfonamide
Helicobacter pylori
pH 7.5, temperature not specified in the publication
1
N-[2-[(2-[1-[(3-amino-3-iminopropyl)amino]ethenyl]-1-methyl-1H-imidazol-4-yl)acetyl]-1-methyl-1H-imidazol-4-yl]-N2-(diaminomethylidene)glycinamide
Staphylococcus aureus
-
pH 7.5, 37°C
1.4
N-[2-[(2-[1-[(3-amino-3-iminopropyl)amino]ethenyl]-1-methyl-1H-imidazol-4-yl)acetyl]-1-methyl-1H-imidazol-4-yl]-N2-(diaminomethylidene)glycinamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.0057
N-[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-N'-(3-phenoxyphenyl)biphenyl-4,4'-dicarboxamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.011
N-[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-N'-(3-phenoxyphenyl)biphenyl-4,4'-dicarboxamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.0045
N4,N4'-bis(3-(1,4,5,6-tetrahydropyrimidin-2-yl)phenyl)biphenyl-4,4'-dicarboxamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.0055
N4,N4'-bis(3-(1,4,5,6-tetrahydropyrimidin-2-yl)phenyl)biphenyl-4,4'-dicarboxamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.0035
N4,N4'-bis(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)biphenyl-4,4'-bis(carbothioamide)
Staphylococcus aureus
-
pH 7.5, 37°C
0.0092
N4,N4'-bis(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)biphenyl-4,4'-bis(carbothioamide)
Staphylococcus aureus
-
pH 7.5, 37°C
0.38
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'- (3-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)phenyl)-[1,1-biphenyl]-4,4-dicarboxamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.53
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'- (3-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)phenyl)-[1,1-biphenyl]-4,4-dicarboxamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.0089
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-(3-(hexyloxy)-phenyl)-[1,1-biphenyl]-4,4-dicarboxamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.025
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-(3-(hexyloxy)-phenyl)-[1,1-biphenyl]-4,4-dicarboxamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.0021
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-(3-methoxyphenyl)-[1,1-biphenyl]-4,4-dicarboxamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.0094
N4-(3-(4,5-dihydro-1H-imidazol-2-yl)phenyl)-N4'-(3-methoxyphenyl)-[1,1-biphenyl]-4,4-dicarboxamide
Staphylococcus aureus
-
pH 7.5, 37°C
0.009
spirohexaline
Staphylococcus aureus
pH 7.5, 37°C
0.009
spirohexaline
Penicillium brasilianum
-
pH and temperature not specified in the publication
0.004
viridicatumtoxin
Staphylococcus aureus
pH 7.5, 37°C
0.004
viridicatumtoxin
Penicillium brasilianum
-
pH and temperature not specified in the publication
0.00036
[1-hydroxy-2-(3-[3-[3-(2-hydroxy-2,2-bis-phosphono-ethyl)-biphenyl-3-ylsulfamoyl]-benzenesulfonylamino]-biphenyl-3-yl)-1-phosphono-ethyl]-phosphonic acid
Staphylococcus aureus
-
pH 7.5, 37°C
0.00039
[1-hydroxy-2-(3-[3-[3-(2-hydroxy-2,2-bis-phosphono-ethyl)-biphenyl-3-ylsulfamoyl]-benzenesulfonylamino]-biphenyl-3-yl)-1-phosphono-ethyl]-phosphonic acid
Staphylococcus aureus
-
pH 7.5, 37°C
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A143V
the C30 intermediate is formed to a greater extent and is longer lived in the process catalyzed by the A69L mutant
A69L
the small side chain of Ala-69 is required for rapid elongation to the C55 product
C234A
kinetik data similar to wild-type. Crystallization data
D190A
-
no significant change
D218A
-
turnover-number is about 9% of the activity of the wild-type enzyme, Km value for farnesyl diphosphate is equal to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is comparable to that of the wild-type enzyme
D223A
-
no significant change
D26E
site-directed mutagenesis, altered Mg2+ binding and over 10000fold reduced activity compared to the wild-type enzyme
D26K
site-directed mutagenesis, altered Mg2+ binding and about 10000fold reduced activity compared to the wild-type enzyme
D26R
site-directed mutagenesis, altered Mg2+ binding and over 10000fold reduced activity compared to the wild-type enzyme
DELTAS72
-
loop-shortening mutant. Change of the length of the loop 72-83 impairs the ability of conformational change and causes remarkably lower activity of UPPs
DELTAS83
-
loop-shortening mutant. Change of the length of the loop 72-83 impairs the ability of conformational change and causes remarkably lower activity of UPPs
DELTAV82S83
-
loop-shortening mutant. Change of the length of the loop 72-83 impairs the ability of conformational change and causes remarkably lower activity of UPPs
E198A
-
no significant change
H103A
the product distributions formed by the use of the I62A, V105A, and H103A mutants are similar to those observed for wild-type UPPs
H199A
-
site-directed mutagenesis, activity is similar to the wild-type enzyme
H199A/E213A
-
site-directed mutagenesis, reduced activity compared to the wild-type enzyme
H43A
-
site-directed mutagenesis, reduced activity compared to the wild-type enzyme
I62A
the product distributions formed by the use of the I62A, V105A, and H103A mutants are similar to those observed for wild-type UPPs
S83(Ala)1
-
mutant with amino acids inserted into the loop
S83(Ala)5
-
mutant with amino acids inserted into the loop
S83A
-
site-directed mutagenesis
V105A
the product distributions formed by the use of the I62A, V105A, and H103A mutants are similar to those observed for wild-type UPPs
W149F
-
turnover-number is 68% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 1.75fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 4.9fold higher than that of the wild-type enzyme
W207F
-
turnover-number is 60% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 8.5fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 5.6fold higher than that of the wild-type enzyme
W221F
-
turnover-number is 140% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 1.3fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 3.4fold higher than that of the wild-type enzyme
W31F
-
turnover-number is 44% of that of the wild-type enzyme,Km-value for farnesyl diphosphate is 5fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 1.4fold higher than that of the wild-type enzyme
W75F
-
turnover-number is 44% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 4.5fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 6.3fold higher than that of the wild-type enzyme
W91F
-
turnover-number is equal to that of the wild-type enzyme, Km-value for farnesyl diphosphate is equal to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 1.8fold higher than that of the wild-type enzyme
A72L
mutation results in shorter ultimate products with C20-35
A72L/F73L
mutation results in shorter ultimate products with C20-35
A72L/F73L/W78L
mutation results in shorter ultimate products with C20-35
D221A
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate within moderate folds to that of the wild-type and slightly decreased enzymatic activity
D226A
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate within moderate folds to that of the wild-type and slightly decreased enzymatic activity
E193Q
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate within moderate folds to that of the wild-type and slightly decreased enzymatic activity
E201Q
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate within moderate folds to that of the wild-type and slightly decreased enzymatic activity
F207S
site-directed mutagenesis, reduced activity, and 13fold increased Km for isopentenyl diphosphate compared to the wild-type enzyme
F223H
dramatically decreased catalytic activity when farnesyl diphosphate is used as allylic substrate
F227S
site-directed mutagenesis
F73L
mutation results in shorter ultimate products with C20-35
N77A
dramatic decrease in catalytic activity, but Km-values for both allylic and homoallylic substrates are comparable to those of the wild-type
N77D
dramatic decrease in catalytic activity, but Km-values for both allylic and homoallylic substrates are comparable to those of the wild-type
N77Q
dramatic decrease in catalytic activity, but Km-values for both allylic and homoallylic substrates are comparable to those of the wild-type
W210A
site-directed mutagenesis, reduced activity, unaltered Km for both substrates compared to the wild-type enzyme
W224A
site-directed mutagenesis, reduced activity, 6fold increased Km for farnesyl diphosphate, and 14fold increased Km for isopentenyl diphosphate compared to the wild-type enzyme
W78D
moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 18.7fold increase in Km-value for farnesyl diphosphate
W78L
mutation results in shorter ultimate products with C20-35
Y148F
site-directed mutagenesis, reduced activity, unaltered Km for both substrates compared to the wild-type enzyme
Y148S
site-directed mutagenesis, inactive mutant
Y71S
site-directed mutagenesis, reduced activity, unaltered Km for both substrates compared to the wild-type enzyme
A72L
-
mutation results in shorter ultimate products with C20-35
-
A72L/F73L/W78L
-
mutation results in shorter ultimate products with C20-35
-
D221A
-
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate within moderate folds to that of the wild-type and slightly decreased enzymatic activity
-
D226A
-
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate within moderate folds to that of the wild-type and slightly decreased enzymatic activity
-
E201Q
-
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate within moderate folds to that of the wild-type and slightly decreased enzymatic activity
-
F207S
-
site-directed mutagenesis, reduced activity, and 13fold increased Km for isopentenyl diphosphate compared to the wild-type enzyme
-
F73A
-
mutation results in shorter ultimate products with C20-35
-
F73L
-
mutation results in shorter ultimate products with C20-35
-
N77A
-
dramatic decrease in catalytic activity, but Km-values for both allylic and homoallylic substrates are comparable to those of the wild-type
-
N77D
-
dramatic decrease in catalytic activity, but Km-values for both allylic and homoallylic substrates are comparable to those of the wild-type
-
N77Q
-
dramatic decrease in catalytic activity, but Km-values for both allylic and homoallylic substrates are comparable to those of the wild-type
-
W210A
-
site-directed mutagenesis, reduced activity, unaltered Km for both substrates compared to the wild-type enzyme
-
W78D
-
moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 18.7fold increase in Km-value for farnesyl diphosphate
-
W78I
-
moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 6-fold increase in Km-value for farnesyl diphosphate, 2.3fold increase in Km-value for (Z,E,E)-geranylgeranyl diphosphate
-
W78L
-
mutation results in shorter ultimate products with C20-35
-
Y148F
-
site-directed mutagenesis, reduced activity, unaltered Km for both substrates compared to the wild-type enzyme
-
Y148S
-
site-directed mutagenesis, inactive mutant
-
Y71S
-
site-directed mutagenesis, reduced activity, unaltered Km for both substrates compared to the wild-type enzyme
-
T86I
-
construction of strain TI392, with resistance to bacitracin, from strain YO-005, which is also modified with the mutation from strain SC26, conferring resistance to scandium, in Bacillus subtilis mutants
T86I
-
construction of strain TI392, with resistance to bacitracin, from strain YO-005, which is also modified with the mutation from strain SC26, conferring resistance to scandium, in Bacillus subtilis mutants
-
D150A
-
turnover-number is 44% of the activity of the wild-type enzyme, Km value for farnesyl diphosphate is equal to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is about 50fold higher than that of the wild-type enzyme
D150A
-
turnover-number is 44% of the activity of the wild-type enzyme, Km value for (2E,6E)-farnesyl diphosphate is equal to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is about 50fold higher than that of the wild-type enzyme
D26A
-
site-directed mutagenesis
D26A
-
turnover-number is less than 1% of that of the wild-type enzyme, Km value for farnesyl diphosphate is comparable to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 3.5fold higher than that of the wild-type enzyme
D26A
-
site-directed mutagenesis, reduced activity compared to the wild-type enzyme
D26A
site-directed mutagenesis, altered Mg2+ binding and about 1000fold reduced activity compared to the wild-type enzyme
D26A
-
the mutant shows altered Mg2+ binding compared to the wild-type enzyme
E213A
-
turnover-number is less than 1% of the activity of the wild-type enzyme, Km value for farnesyl diphosphate is 1.75fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is about 68.3fold higher than that of the wild-type enzyme
E213A
-
site-directed mutagenesis, reduced activity compared to the wild-type enzyme
E73A
-
turnover-number is 12% of that of the wild-type enzyme, Km value for farnesyl diphosphate is equal to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is about 4fold higher than that of the wild-type enzyme
E73A
turnover-number is 12% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is equal to that of the wild-type enzyme, the Km-value for isopentenyl diphosphate is 3.9fold higher than that of the wild-type enzyme
E81A
turnover-number is 16% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is equal to that of the wild-type enzyme, the Km-value for isopentenyl diphosphate is 21.5fold higher than that of the wild-type enzyme
E81A
increases in the IPP Km values
F89A
-
site-directed mutagenesis, mutant shows highly increased Km for isopentenyl diphosphate with farnesyl diphosphate and slightly with geranylgeranyl diphosphate compared to the wild-type enzyme
F89A
-
mutation increases (2E,6E)-farnesyl diphosphate and geranylgeranyl diphosphate Km values, indicating that these amino acids are important for substrate binding, but do not determine substrate specificity
L137A
mutant enzyme produces C55-polyprenyl diphosphate, C60-polyprenyl diphosphate and C65-polyprenyl diphosphate in the ratio 55:41:4 in presence of Triton, compared to the wild-type enzyme which produces C55-polyprenyl diphosphate as the major product. In absence of Triton the mutant enzyme produces C70 and C75-polyprenyl diphosphate is the major products
L137A
catalysis results in predominantly the formation of the C70 polymer rather than the C55 polymer
L85A
-
site-directed mutagenesis, mutant shows highly increased Km for isopentenyl diphosphate with farnesyl diphosphate and slightly with geranylgeranyl diphosphate compared to the wild-type enzyme
L85A
-
mutation increases (2E,6E)-farnesyl diphosphate and geranylgeranyl diphosphate Km values, indicating that these amino acids are important for substrate binding, but do not determine substrate specificity
L88A
-
site-directed mutagenesis, mutant shows highly increased Km for isopentenyl diphosphate with farnesyl diphosphate and slightly with geranylgeranyl diphosphate compared to the wild-type enzyme
L88A
-
mutation increases (2E,6E)-farnesyl diphosphate and geranylgeranyl diphosphate Km values, indicating that these amino acids are important for substrate binding, but do not determine substrate specificity
N74A
turnover-number is less than 1% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is equal to that of the wild-type enzyme, the Km-value for isopentenyl diphosphate is 2fold higher than that of the wild-type enzyme
N74A
decrease in kcat values
R77A
turnover-number is less than 1% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 4fold higher than that of the wild-type enzyme, the Km-value for isopentenyl diphosphate is 3.8fold higher than that of the wild-type enzyme
R77A
decrease in kcat values
S71A
turnover-number is 4.4% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 2.5fold higher than that of the wild-type enzyme, the Km-value for isopentenyl diphosphate is 32.4fold higher than that of the wild-type enzyme
S71A
decrease in kcat values
S71A
increases in the IPP Km values
W75A
turnover-number is 4.4% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 8fold higher than that of the wild-type enzyme, the Km-value for isopentenyl diphosphate is 11.2fold higher than that of the wild-type enzyme
W75A
shows 8fold increase of the FPP Km value
C234A
kinetic data similar to wild-type. Crystallization data
C234A
C234A UPPS mutant is crystallized using the hanging drop method, C234A has unchanged kinetic properties compared to wild-type
C234A
site-directed mutagenesis, mutation to prevent intramolecular disulfide bond formed during the long period of crystallization process
D29A
Km-value for farnesyl diphosphate is 9.2fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 5.8fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 1% of that of the wild-type enzyme
D29A
Km-value for farnesyl diphosphate is 9.2fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 5.8fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 1% of that of the wild-type enzyme
E216Q
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate is about 11.6fold higher than that for the wild-type enzyme, turnover number is about 18fold lower. The major products contain C35 and C50 prenyl chain length
E216Q
site-directed mutagenesis, Km value increased
E76Q
Km-value for farnesyl diphosphate is 1.6fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 3.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.5fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 30% of that of the wild-type enzyme
E76Q
Km-value for farnesyl diphosphate is 1.6fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 3.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.5fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 30% of that of the wild-type enzyme
E84Q
Km-value for farnesyl diphosphate is 3.2fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.98fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 65% of that of the wild-type enzyme
E84Q
Km-value for farnesyl diphosphate is 3.2fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.98fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 65% of that of the wild-type enzyme
F73A
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. 32fold increase in Km-value for isopentenyl diphosphate and about 17fold decrease in turnover-number. A mixture of C30 and C50 products is formed
F73A
mutation results in shorter ultimate products with C20-35
F73A
yielded shorter chain prenyl diphosphates as their main products
G32R
Km-value for farnesyl diphosphate is comparable to that of the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.9fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is comparable to that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 27% of that of the wild-type enzyme
G32R
Km-value for farnesyl diphosphate is comparable to that of the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.9fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is comparable to that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 27% of that of the wild-type enzyme
G32R/R42G
Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.4fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 58% of that of the wild-type enzyme
G32R/R42G
Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.4fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 58% of that of the wild-type enzyme
R197S
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate is about 7.2fold higher than that for the wild-type enzyme, turnover-number is 1200fold lower. Compared with the wild-type enzyme lower reaction rate in catalysis and fewer product produced after 6 h reaction
R197S
site-directed mutagenesis, Km value increased
R203S
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate is about 8fold higher than that for the wild-type enzyme, turnover-number is 1200fold lower. Compared with the wild-type enzyme lower reaction rate in catalysis and fewer product produced after 6 h reaction
R203S
site-directed mutagenesis, Km value increased
R33A
Km-value for farnesyl diphosphate is 40fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.2fold lower than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 3% of that of the wild-type enzyme. Different product distribution pattern compared to the wildf-type enzyme
R33A
Km-value for farnesyl diphosphate is 40fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.2fold lower than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 3% of that of the wild-type enzyme. Different product distribution pattern compared to the wildf-type enzyme
R42G
Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.8fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.1fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 13% of that of the wild-type enzyme
R42G
Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.8fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.1fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 13% of that of the wild-type enzyme
R80A
Km-value for farnesyl diphosphate is 6.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 6.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is less than 1% of that of the wild-type enzyme
R80A
Km-value for farnesyl diphosphate is 6.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 6.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is less than 1% of that of the wild-type enzyme
S74A
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. 16fold increase in Km-value for isopentenyl diphosphate and about 12fold decrease in turnover-number. The major products contain C35 and C50 prenyl chain length
S74A
yielded shorter chain prenyl diphosphates as their main products
W78I
moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 6-fold increase in Km-value for farnesyl diphosphate, 2.3fold increase in Km-value for (Z,E,E)-geranylgeranyl diphosphate
W78I
moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 6-fold increase in Km-value for farnesyl diphosphate, 2.3fold increase in Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate
W78R
moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 14.5-fold increase in Km-value for farnesyl diphosphate, 1.8fold increase in Km-value for (Z,E,E)-geranylgeranyl diphosphate
W78R
moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 14.5-fold increase in Km-value for farnesyl diphosphate, 1.8fold increase in Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate
D29A
-
Km-value for farnesyl diphosphate is 9.2fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 5.8fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 1% of that of the wild-type enzyme
-
D29A
-
Km-value for farnesyl diphosphate is 9.2fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 5.8fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 1% of that of the wild-type enzyme
-
E76Q
-
Km-value for farnesyl diphosphate is 1.6fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 3.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.5fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 30% of that of the wild-type enzyme
-
E76Q
-
Km-value for farnesyl diphosphate is 1.6fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 3.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.5fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 30% of that of the wild-type enzyme
-
E84Q
-
Km-value for farnesyl diphosphate is 3.2fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.98fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 65% of that of the wild-type enzyme
-
E84Q
-
Km-value for farnesyl diphosphate is 3.2fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.98fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 65% of that of the wild-type enzyme
-
G32R
-
Km-value for farnesyl diphosphate is comparable to that of the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.9fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is comparable to that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 27% of that of the wild-type enzyme
-
G32R
-
Km-value for farnesyl diphosphate is comparable to that of the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.9fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is comparable to that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 27% of that of the wild-type enzyme
-
G32R/R42G
-
Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.4fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 58% of that of the wild-type enzyme
-
G32R/R42G
-
Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.4fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 58% of that of the wild-type enzyme
-
S74A
-
comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. 16fold increase in Km-value for isopentenyl diphosphate and about 12fold decrease in turnover-number. The major products contain C35 and C50 prenyl chain length
-
S74A
-
yielded shorter chain prenyl diphosphates as their main products
-
additional information
-
gene uppS1 contains a single-point mutation in the ribosome-binding site of the uppS gene. The uppS1 allele is sufficient to confer low-level vancomycin resistance and causes reduced UppS translation. The decreased level of UppS renders Bacillus subtilis slightly more susceptible to many late-acting cell wall antibiotics, including beta-lactams, but significantly more resistant to fosfomycin and D-cycloserine, antibiotics that interfere with the very early steps of cell wall synthesis. The uppS1 allele leads to slightly elevated expression of the sigmaM regulon, possibly helping to compensate for the stress caused by a decrease in UPP levels
additional information
-
gene uppS1 contains a single-point mutation in the ribosome-binding site of the uppS gene. The uppS1 allele is sufficient to confer low-level vancomycin resistance and causes reduced UppS translation. The decreased level of UppS renders Bacillus subtilis slightly more susceptible to many late-acting cell wall antibiotics, including beta-lactams, but significantly more resistant to fosfomycin and D-cycloserine, antibiotics that interfere with the very early steps of cell wall synthesis. The uppS1 allele leads to slightly elevated expression of the sigmaM regulon, possibly helping to compensate for the stress caused by a decrease in UPP levels
-
additional information
-
loop-shortening mutants with deletion of V82 and S83 or S72 also adopt an open conformation with the loop stretched, although they show decreased intrinsic fluorescence with (2E,6E)-farnesyl diphosphate bound, similar to that seen in the wild-type enzyme
additional information
-
four uppS deletion mutants of Streptococcus pneumoniae R6 pKO1-4
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Muth, J.D.; Allen, C.M.
Undecaprenyl pyrophosphate synthetase from Lactobacillus plantarum: a dimeric protein
Arch. Biochem. Biophys.
230
49-60
1984
Lactiplantibacillus plantarum, Lactiplantibacillus plantarum (Q88VJ8)
brenda
Takahashi, I.; Ogura, K.
Prenyltransferases of Bacillus subtilis: undecaprenyl pyrophosphate synthetase and geranylgeranyl pyrophosphate synthetase
J. Biochem.
92
1527-1537
1982
Bacillus subtilis
brenda
Allen, C.M.
Purification and characterization of undecaprenylpyrophosphate synthetase
Methods Enzymol.
110
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