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Literature summary for 2.5.1.31 extracted from

  • Lee, Y.; Helmann, J.
    Reducing the level of undecaprenyl pyrophosphate synthase has complex effects on susceptibility to cell wall antibiotics (2013), Antimicrob. Agents Chemother., 57, 4267-4275.
No PubMed abstract available

Application

Application Comment Organism
drug development enzyme UppS is an attractive antibacterial target, but incomplete inhibition of UppS function may lead to increased resistance to some cell wall-active antibiotics Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
gene uppS1 containing a single-point mutation in the ribosome-binding site of the uppS gene, resulting in mutant strain HB13564, genotyping, DNA and amino acid sequence determination and analysis Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information gene uppS1 contains a single-point mutation in the ribosome-binding site of the uppS gene. The uppS1 allele is sufficient to confer low-level vancomycin resistance and causes reduced UppS translation. The decreased level of UppS renders Bacillus subtilis slightly more susceptible to many late-acting cell wall antibiotics, including beta-lactams, but significantly more resistant to fosfomycin and D-cycloserine, antibiotics that interfere with the very early steps of cell wall synthesis. The uppS1 allele leads to slightly elevated expression of the sigmaM regulon, possibly helping to compensate for the stress caused by a decrease in UPP levels Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
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gene uppS1 containing a single-point mutation in the ribosome-binding site of the uppS gene, mutant strain HB13564
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Bacillus subtilis W168
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gene uppS1 containing a single-point mutation in the ribosome-binding site of the uppS gene, mutant strain HB13564
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Synonyms

Synonyms Comment Organism
undecaprenyl pyrophosphate synthase
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Bacillus subtilis
UPPs
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Bacillus subtilis

General Information

General Information Comment Organism
malfunction the uppS1 mutation increases resistance to vancomycin, fosfomycin, and D-cycloserine in wild-type cells, but this effect is greatly reduced or eliminated in a sigmaM mutant background. Incomplete inhibition of UppS function may lead to increased resistance to some cell wall-active antibiotics. Phenotype, overview Bacillus subtilis