Cloned (Comment) | Organism |
---|---|
gene uppS, recombinant His-tagged enzyme expression in Escherichia coli strain BL21 | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme free or in complex with substrate (2E,6E)-farnesyl diphosphate, X-ray diffraction structure determination and analysis at resolutions of 2.2 A and 2.1A, respectively | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(2-chlorophenyl)-N-(4-isopropylphenyl-5-methylisoxazole-4-carboxamide) | i.e. UK-106051, a drug-like UPPs inhibitor, UK-106051 binds to a site in the middle of this binding cleft. Substrates FPP and IPP cannot bind simultaneously to UPPs-inhibitor binary complexes | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | Streptococcus pneumoniae | - |
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | Q8DRB3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Streptococcus pneumoniae | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
undecaprenyl pyrophosphate synthase | - |
Streptococcus pneumoniae |
UPPs | - |
Streptococcus pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptococcus pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptococcus pneumoniae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00005 | - |
pH 7.5, 37°C | Streptococcus pneumoniae | 3-(2-chlorophenyl)-N-(4-isopropylphenyl-5-methylisoxazole-4-carboxamide) |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is an essential enzyme in a key bacterial cell wall synthesis pathway. It catalyzes the consecutive condensations of isopentenyl diphosphate groups on to a trans-farnesyl diphosphate to produce the C55 isoprenoid undecaprenyl diphosphate | Streptococcus pneumoniae |