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Literature summary for 2.5.1.31 extracted from

  • Pan, J.J.; Chiou, S.T.; Liang, P.H.
    Product distribution and pre-steady-state kinetic analysis of Escherichia coli undecaprenyl pyrophosphate synthase reaction (2000), Biochemistry, 39, 10936-10942.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Phospholipid
-
Escherichia coli
Triton 0.01 microM of UPPs with 5 microM of (2E,6E)-farnesyl diphosphate and 50 microM of isopentenyl diphosphate, 0.5 mM MgCl2, and 50 mM KCl in Hepes buffer (pH 7.5) is 190fold less active (0.013 s-1) for the IPP consumption in the absence of Triton Escherichia coli
Triton the UPPs steady-state kcat value in the presence of 0.1% Triton is 190fold larger than in the absence of Triton Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
(2E,6E)-farnesyl diphosphate pH 7.5, 25°C Escherichia coli
0.0004
-
(2E,6E)-farnesyl diphosphate wild-type enzyme, pH 7.5, 25°C Escherichia coli
0.0004
-
farnesyl diphosphate wild-type enzyme, pH 7.5, 25°C Escherichia coli
0.0041
-
isopentenyl diphosphate pH 7.5, 25°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependend on Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate Escherichia coli catalyzes the condensation of eight molecules of isopentenyl pyrophosphate (IPP) with farnesyl diphosphate (FPP) to generate C55 undecaprenyl diphosphate. With a variety of different ratios of enzyme to substrate and FPP to IPP in the presence or absence of Triton, different product distributions are found 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli
His-tagged UPPs Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.013
-
pH 7.5, 25°C, absence of Triton Escherichia coli
2.5
-
pH 7.5, 25°C, in presence of 0.1% Triton Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
Escherichia coli 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
Escherichia coli 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate quantification of product distribution of UPPs catalyzed coupling of (2E,6E)-farnesyl diphosphate and isopentenyl diphosphate under various reaction conditions ?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate catalyzes the condensation of eight molecules of isopentenyl pyrophosphate (IPP) with farnesyl diphosphate (FPP) to generate C55 undecaprenyl diphosphate. With a variety of different ratios of enzyme to substrate and FPP to IPP in the presence or absence of Triton, different product distributions are found Escherichia coli 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
isopentenyl diphosphate + farnesyl diphosphate
-
Escherichia coli C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate product distribution under various reaction conditions. In the presence of excess farnesyl diphosphate the intermediates C25-C50 accumulate ?

Synonyms

Synonyms Comment Organism
undecaprenyl pyrophosphate synthase
-
Escherichia coli
UPPs
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
(2E,6E)-farnesyl diphosphate pH 7.5, 25°C, without 0.1% Triton Escherichia coli
2.5
-
(2E,6E)-farnesyl diphosphate pH 7.5, 25°C, in the presence of 0.1% Triton Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli