Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.31 extracted from

  • Dodbele, S.; Martinez, C.D.; Troutman, J.M.
    Species differences in alternative substrate utilization by the antibacterial target undecaprenyl pyrophosphate synthase (2014), Biochemistry, 53, 5042-5050.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene upps, recombinant expression of His-tagged enzyme in Escherichia coli strain C41 Escherichia coli
gene upps, recombinant expression of His-tagged enzyme in Escherichia coli strain C41 Bacteroides fragilis
gene upps, recombinant expression of His-tagged enzyme in Escherichia coli strain C41 Vibrio vulnificus

Inhibitors

Inhibitors Comment Organism Structure
2-propanol
-
Bacteroides fragilis
2-propanol
-
Escherichia coli
2-propanol
-
Vibrio vulnificus
diphosphate product inhibition Bacteroides fragilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli
Mg2+ required Bacteroides fragilis
Mg2+ required Vibrio vulnificus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate Escherichia coli
-
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate Bacteroides fragilis
-
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate Vibrio vulnificus
-
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate Escherichia coli DH5alpha
-
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate Vibrio vulnificus MO6-24/O
-
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate Bacteroides fragilis ATCC 25285
-
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis Q5LI17
-
-
Bacteroides fragilis ATCC 25285 Q5LI17
-
-
Escherichia coli P60472
-
-
Escherichia coli DH5alpha P60472
-
-
Vibrio vulnificus A0A087IPJ7
-
-
Vibrio vulnificus MO6-24/O A0A087IPJ7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain C41 by nickel affinity chromatography, dialysis, and gel filtration Escherichia coli
recombinant His-tagged enzyme from Escherichia coli strain C41 by nickel affinity chromatography, dialysis, and gel filtration Bacteroides fragilis
recombinant His-tagged enzyme from Escherichia coli strain C41 by nickel affinity chromatography, dialysis, and gel filtration Vibrio vulnificus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
Escherichia coli 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
Bacteroides fragilis 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
Vibrio vulnificus 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
Escherichia coli DH5alpha 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
Vibrio vulnificus MO6-24/O 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
Bacteroides fragilis ATCC 25285 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
2-amideanilinogeranyl diphosphate + isopentenyl diphosphate lower activity, the fluorescent substrate analogue undergoes a 1.2fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays Bacteroides fragilis ? ESI-MS analysis suggests that these major product peaks correspond to six to nine Z-configuration isoprenes incorporated by the UPPS protein ?
2-amideanilinogeranyl diphosphate + isopentenyl diphosphate lower activity, the fluorescent substrate analogue undergoes a 1.2fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays Bacteroides fragilis ATCC 25285 ? ESI-MS analysis suggests that these major product peaks correspond to six to nine Z-configuration isoprenes incorporated by the UPPS protein ?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays Escherichia coli 9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
-
?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays Bacteroides fragilis 9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
-
?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays Vibrio vulnificus 9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
-
?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays Escherichia coli DH5alpha 9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
-
?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays Vibrio vulnificus MO6-24/O 9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
-
?
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays Bacteroides fragilis ATCC 25285 9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate
-
?
additional information development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity Bacteroides fragilis ?
-
?
additional information development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate Escherichia coli ?
-
?
additional information development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate Vibrio vulnificus ?
-
?
additional information development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate Escherichia coli DH5alpha ?
-
?
additional information development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate Vibrio vulnificus MO6-24/O ?
-
?
additional information development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity Bacteroides fragilis ATCC 25285 ?
-
?

Subunits

Subunits Comment Organism
homodimer
-
Escherichia coli
homodimer
-
Bacteroides fragilis
homodimer
-
Vibrio vulnificus

Synonyms

Synonyms Comment Organism
undecaprenyl pyrophosphate synthase
-
Escherichia coli
undecaprenyl pyrophosphate synthase
-
Bacteroides fragilis
undecaprenyl pyrophosphate synthase
-
Vibrio vulnificus
UPPs
-
Escherichia coli
UPPs
-
Bacteroides fragilis
UPPs
-
Vibrio vulnificus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli
37
-
assay at Bacteroides fragilis
37
-
assay at Vibrio vulnificus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
assay at Escherichia coli
8.3
-
assay at Bacteroides fragilis
8.3
-
assay at Vibrio vulnificus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1
-
pH 8.3, 37°C Bacteroides fragilis diphosphate
7.4
-
pH 8.3, 37°C, in presence of 2-nitrileanilinogeranyl diphosphate Bacteroides fragilis diphosphate

General Information

General Information Comment Organism
physiological function bactoprenyl diphosphate (BPP), a two-E eight-Z configuration C55 isoprenoid, serves as a critical anchor for the biosynthesis of complex glycans central to bacterial survival and pathogenesis. BPP is formed by the polymerase undecaprenyl diphosphate synthase (UppS), which catalyzes the elongation of a single farnesyl diphosphate (FPP) with eight Z-configuration isoprene units from eight isopentenyl diphosphates Escherichia coli
physiological function bactoprenyl diphosphate (BPP), a two-E eight-Z configuration C55 isoprenoid, serves as a critical anchor for the biosynthesis of complex glycans central to bacterial survival and pathogenesis. BPP is formed by the polymerase undecaprenyl diphosphate synthase (UppS), which catalyzes the elongation of a single farnesyl diphosphate (FPP) with eight Z-configuration isoprene units from eight isopentenyl diphosphates Bacteroides fragilis
physiological function bactoprenyl diphosphate (BPP), a two-E eight-Z configuration C55 isoprenoid, serves as a critical anchor for the biosynthesis of complex glycans central to bacterial survival and pathogenesis. BPP is formed by the polymerase undecaprenyl diphosphate synthase (UppS), which catalyzes the elongation of a single farnesyl diphosphate (FPP) with eight Z-configuration isoprene units from eight isopentenyl diphosphates Vibrio vulnificus