Cloned (Comment) | Organism |
---|---|
gene upps, recombinant expression of His-tagged enzyme in Escherichia coli strain C41 | Escherichia coli |
gene upps, recombinant expression of His-tagged enzyme in Escherichia coli strain C41 | Bacteroides fragilis |
gene upps, recombinant expression of His-tagged enzyme in Escherichia coli strain C41 | Vibrio vulnificus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-propanol | - |
Bacteroides fragilis | |
2-propanol | - |
Escherichia coli | |
2-propanol | - |
Vibrio vulnificus | |
diphosphate | product inhibition | Bacteroides fragilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
Mg2+ | required | Bacteroides fragilis | |
Mg2+ | required | Vibrio vulnificus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | Escherichia coli | - |
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | Bacteroides fragilis | - |
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | Vibrio vulnificus | - |
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | Escherichia coli DH5alpha | - |
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | Vibrio vulnificus MO6-24/O | - |
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | Bacteroides fragilis ATCC 25285 | - |
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | Q5LI17 | - |
- |
Bacteroides fragilis ATCC 25285 | Q5LI17 | - |
- |
Escherichia coli | P60472 | - |
- |
Escherichia coli DH5alpha | P60472 | - |
- |
Vibrio vulnificus | A0A087IPJ7 | - |
- |
Vibrio vulnificus MO6-24/O | A0A087IPJ7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain C41 by nickel affinity chromatography, dialysis, and gel filtration | Escherichia coli |
recombinant His-tagged enzyme from Escherichia coli strain C41 by nickel affinity chromatography, dialysis, and gel filtration | Bacteroides fragilis |
recombinant His-tagged enzyme from Escherichia coli strain C41 by nickel affinity chromatography, dialysis, and gel filtration | Vibrio vulnificus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Escherichia coli | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Bacteroides fragilis | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Vibrio vulnificus | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Escherichia coli DH5alpha | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Vibrio vulnificus MO6-24/O | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Bacteroides fragilis ATCC 25285 | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
2-amideanilinogeranyl diphosphate + isopentenyl diphosphate | lower activity, the fluorescent substrate analogue undergoes a 1.2fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays | Bacteroides fragilis | ? | ESI-MS analysis suggests that these major product peaks correspond to six to nine Z-configuration isoprenes incorporated by the UPPS protein | ? | |
2-amideanilinogeranyl diphosphate + isopentenyl diphosphate | lower activity, the fluorescent substrate analogue undergoes a 1.2fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays | Bacteroides fragilis ATCC 25285 | ? | ESI-MS analysis suggests that these major product peaks correspond to six to nine Z-configuration isoprenes incorporated by the UPPS protein | ? | |
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate | the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays | Escherichia coli | 9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate | - |
? | |
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate | the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays | Bacteroides fragilis | 9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate | - |
? | |
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate | the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays | Vibrio vulnificus | 9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate | - |
? | |
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate | the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays | Escherichia coli DH5alpha | 9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate | - |
? | |
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate | the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays | Vibrio vulnificus MO6-24/O | 9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate | - |
? | |
2-nitrileanilinogeranyl diphosphate + 9 isopentenyl diphosphate | the fluorescent substrate analogue undergoes a 2.5fold increase in fluorescence upon isoprenoid chain elongation, and this increase in fluorescence can be utilized to monitor the activity and inhibition of UPPS in 96-well plate assays | Bacteroides fragilis ATCC 25285 | 9 diphosphate + 2-nitrileanilinobactoprenyl diphosphate | - |
? | |
additional information | development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity | Bacteroides fragilis | ? | - |
? | |
additional information | development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate | Escherichia coli | ? | - |
? | |
additional information | development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate | Vibrio vulnificus | ? | - |
? | |
additional information | development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate | Escherichia coli DH5alpha | ? | - |
? | |
additional information | development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity. No activity with 2-amideanilinogeranyl diphosphate due to competition with diphosphate | Vibrio vulnificus MO6-24/O | ? | - |
? | |
additional information | development of assay methods for UPPS, the difference in substrate utilization between forms of UPPS from different species has major implications for UPPS inhibitor development, assay construction, and the development of polysaccharide biosynthesis probes. 2-Nitrileanilinogeranyl diphosphate (2CNA-GPP) is a general and effective continuous probe for UPPS activity | Bacteroides fragilis ATCC 25285 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Escherichia coli |
homodimer | - |
Bacteroides fragilis |
homodimer | - |
Vibrio vulnificus |
Synonyms | Comment | Organism |
---|---|---|
undecaprenyl pyrophosphate synthase | - |
Escherichia coli |
undecaprenyl pyrophosphate synthase | - |
Bacteroides fragilis |
undecaprenyl pyrophosphate synthase | - |
Vibrio vulnificus |
UPPs | - |
Escherichia coli |
UPPs | - |
Bacteroides fragilis |
UPPs | - |
Vibrio vulnificus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Bacteroides fragilis |
37 | - |
assay at | Vibrio vulnificus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
assay at | Escherichia coli |
8.3 | - |
assay at | Bacteroides fragilis |
8.3 | - |
assay at | Vibrio vulnificus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1 | - |
pH 8.3, 37°C | Bacteroides fragilis | diphosphate | |
7.4 | - |
pH 8.3, 37°C, in presence of 2-nitrileanilinogeranyl diphosphate | Bacteroides fragilis | diphosphate |
General Information | Comment | Organism |
---|---|---|
physiological function | bactoprenyl diphosphate (BPP), a two-E eight-Z configuration C55 isoprenoid, serves as a critical anchor for the biosynthesis of complex glycans central to bacterial survival and pathogenesis. BPP is formed by the polymerase undecaprenyl diphosphate synthase (UppS), which catalyzes the elongation of a single farnesyl diphosphate (FPP) with eight Z-configuration isoprene units from eight isopentenyl diphosphates | Escherichia coli |
physiological function | bactoprenyl diphosphate (BPP), a two-E eight-Z configuration C55 isoprenoid, serves as a critical anchor for the biosynthesis of complex glycans central to bacterial survival and pathogenesis. BPP is formed by the polymerase undecaprenyl diphosphate synthase (UppS), which catalyzes the elongation of a single farnesyl diphosphate (FPP) with eight Z-configuration isoprene units from eight isopentenyl diphosphates | Bacteroides fragilis |
physiological function | bactoprenyl diphosphate (BPP), a two-E eight-Z configuration C55 isoprenoid, serves as a critical anchor for the biosynthesis of complex glycans central to bacterial survival and pathogenesis. BPP is formed by the polymerase undecaprenyl diphosphate synthase (UppS), which catalyzes the elongation of a single farnesyl diphosphate (FPP) with eight Z-configuration isoprene units from eight isopentenyl diphosphates | Vibrio vulnificus |