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2.7.7.23: UDP-N-acetylglucosamine diphosphorylase

This is an abbreviated version!
For detailed information about UDP-N-acetylglucosamine diphosphorylase, go to the full flat file.

Word Map on EC 2.7.7.23

Reaction

UTP
+
N-acetyl-alpha-D-glucosamine 1-phosphate
=
diphosphate
 +
UDP-N-acetyl-alpha-D-glucosamine

Synonyms

acetylglucosamine 1-phosphate uridylyltransferase, AGX-1, AGX1, AGX2, amino-sugar-1-phosphate acetyltransferase, bifunctional N-acetyltransferase/uridylyltransferase, BmUAP, CL6EHI_021200, CL6EHI_039830, EcGlmU, EnhiA.01126.a, EnhiA.01126.b, GlcNAc-1-P uridyltransferase, GlcNAc-1-P UTase, GlcNAc-1-phosphate nucleotidylyltransferase, GLCNAC1PUT1, GLCNAC1PUT2, GlmU, GlmUMTB, hiGlmU, LmUAP1, LmUAP2, LOC_Os08g10600, Mmy, More, mummy, N-acetyl-D-glucosamine-1-phosphate uridylyltransferase, N-acetyl-glucosamine-1-phosphate uridyltransferase, N-acetylglucosamine 1-phosphate uridyltransferase, N-acetylglucosamine-1-phosphate uridyltransferase, N-acetylglucosamine-1-phosphate uridylyltransferase, N-acetylglucosamine-1-phosphate-uridyltransferase/glucosamine-1-phosphate-acetyltransferase, Os08g10600, Rv1018c, spl29, spotted leaf 29, ST0452, ST0452 protein, STK_04520, Tb11.02.0120, UAP, UAP enzyme, UAP1, UAP2, UDP N-acetylglucosamine pyrophosphorylases 1, UDP N-acetylglucosamine pyrophosphorylases 2, UDP-GlcNAc pyrophosphorylase, UDP-GlcNAc pyrophosphorylase (UAP), UDP-HexNAc pyrophosphorylase, UDP-N-acetylgalactosamine pyrophosphorylase, UDP-N-acetylglucosamine diphosphorylase 1, UDP-N-acetylglucosamine diphosphorylase 2, UDP-N-acetylglucosamine pyrophosphorylase, UDP-N-acetylglucosamine pyrophosphorylase (UAP), UDP-N-acetylglucosamine pyrophosphorylase 1, UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase, UDP-N-acetylglucosamine-diphosphorylase, UDP-N-acetylhexosamine pyrophosphorylase, UDPacetylglucosamine pyrophosphorylase, UNAcP, uridine diphosphate N-acetylglucosamine pyrophosphorylase, uridine diphosphate-N-acetylglucosamine pyrophosphorylase, uridine diphosphoacetylglucosamine phosphorylase, uridine diphosphoacetylglucosamine pyrophosphorylase, uridine-diphospho-N-acetylglucosamine pyrophosphorylase, UTP:2-acetamido-2-deoxy-alpha-D-glucose-1-phosphate uridylyltransferase, UTP:N-acetylglucosamine-1-P uridylyltransferase, Ydl103c protein

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.23 UDP-N-acetylglucosamine diphosphorylase

Cloned

Cloned on EC 2.7.7.23 - UDP-N-acetylglucosamine diphosphorylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AGX1 and AGX 2 expressed in Escherichia coli as GST fusion protein
-
AGX1 expressed in Escherichia coli as GST fusion protein
-
expressed as His-tag fusion protein in Escherichia coli M15
expressed in Escherichia coli
expressed in Escherichia coli as GST fusion protein, complementation of Saccharomyces cerevisiae deficiency mutant, all recombinant enzymes found to be active
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli DH5alpha and BL21 (DE3) cells
expressed in Escherichia coli JM109 cells as a glutathione S-transferase fusion protein
-
expressed in M15 cells
expression in Escherichia coli
expression in Escherichia coli BL21-Codon Plus (DE3)-RIL cells
expression in Escherichia coli, wild-type and truncated enzyme form lacking the 170-residues C-terminal domain
expression of mutant enzymes in Escherichia coli
gene CL6EHI_021200, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli BL21(DE3)
gene CL6EHI_039830, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli BL21(DE3)
gene glmU, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene glmU, DNA and amino acid sequence determination, analysis, and comparison, functional complementation of the Escherichia coli glmU thermosensitive mutant strain UGS83, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene glmU, recombinant expression of His-tagged enzyme in Escherichia coli strain BL12(DE3), overexpression of GlmU in Mycobacterium tuberculosis
gene glmU, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene glmU, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene glmU, recombinant expression of N-terminally FLAG-tagged enzyme
gene LdUAP1, DNA and amino acid sequence determination and analysis, quantitative real-time quantitative PCR expression analysis
KT282116, KT282117
gene LdUAP2, DNA and amino acid sequence determination and analysis, quantitative real-time quantitative PCR expression analysis
KT282116, KT282117
gene LmUAP1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
gene LmUAP2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
gene mmy, quantitative RT-PCR expression analysis
gene SPL29, map-based cloning, DNA and amino acid sequence determination and analysis, gene sequence and structure of SPL29, phylogenetic tree, recombinant expression of gene SPL29 and mutant spl29 in callus via Agrobacterium tumefaciens strain EHA105 transfection, recombinant expression fo GST-tagged wild-type and mutant SPL29 in Escherichia coli
gene ST0452, recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-Codon Plus(DE3)-RIL
gene ST0452, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL, subcloning in Escherichia coli strain JM109
gene UAP1 encodes two different isoforms, named AGX1 and AGX2, recombinant expression of N-terminally GST-tagged wild-type and mutant AGX1 in Escherichia coli strain (DE3) pLysS
native and mutant enzymes expressed as His-tag fusion protein in Escherichia coli M15
overexpressed in Escherichia coli, His-tag inserted for purification
-
overexpression in host strain
-
recombinant expression of His-tagged enzyme and of GST-tagged in Escherichia coli strain BL21(DE3)
-
recombinant expression of His-tagged wild-type enzyme and mutant Y97N in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
replacement of several amino acids, GST fusion protein
truncated and full length enzyme overexpressed in Escherichia coli, His-tag inserted for purification
-