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Literature summary for 2.7.7.23 extracted from
- The eukaryotic UDP-N-acetylglucosamine pyrophosphorylases. Gene cloning, protein expression, and catalytic mechanism (1998), J. Biol. Chem., 273, 14392-14397.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as GST fusion protein, complementation of Saccharomyces cerevisiae deficiency mutant, all recombinant enzymes found to be active | Homo sapiens |
| expressed in Escherichia coli as GST fusion protein, complementation of Saccharomyces cerevisiae deficiency mutant, all recombinant enzymes found to be active | Candida albicans |
| replacement of several amino acids, GST fusion protein | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G111A | very low activity | Homo sapiens |
| G111A | very low activity | Saccharomyces cerevisiae |
| G112A | traces of activity | Saccharomyces cerevisiae |
| G114A | 25% of activity | Saccharomyces cerevisiae |
| K123A | traces of activity | Saccharomyces cerevisiae |
| L117A | very low activity | Saccharomyces cerevisiae |
| P122 A | 35% of activity | Saccharomyces cerevisiae |
| R116A | traces of activity | Saccharomyces cerevisiae |
| T115A | 50% of activity | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
N-acetyl-D-glucosamine 1-phosphate | R116A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae |  |
| 0.012 | - |
N-acetyl-D-glucosamine 1-phosphate | P122A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 0.014 | - |
N-acetyl-D-glucosamine 1-phosphate | wild type enzyme, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 0.015 | - |
N-acetyl-D-glucosamine 1-phosphate | T115A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 0.018 | - |
N-acetyl-D-glucosamine 1-phosphate | K123A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 0.019 | - |
N-acetyl-D-glucosamine 1-phosphate | G114A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 0.029 | - |
N-acetyl-D-glucosamine 1-phosphate | L117A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 0.034 | - |
N-acetyl-D-glucosamine 1-phosphate | G111A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 0.124 | - |
N-acetyl-D-glucosamine 1-phosphate | G112A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-N-acetyl-D-glucosamine | Homo sapiens | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | Saccharomyces cerevisiae | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | Candida albicans | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | Homo sapiens | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | Saccharomyces cerevisiae | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | Candida albicans | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | Saccharomyces cerevisiae | deficiency mutant fully swollen and some are lysed | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | O74933 | - |
- |
| Homo sapiens | - |
- |
- |
| Saccharomyces cerevisiae | P43123 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GST fusion protein from E. coli | Homo sapiens |
| GST fusion protein from E. coli | Saccharomyces cerevisiae |
| GST fusion protein from E. coli | Candida albicans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| testis | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-glucose | - |
Homo sapiens | UTP + D-glucose 1-phosphate | - |
r | |
| diphosphate + UDP-glucose | - |
Candida albicans | UTP + D-glucose 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | - |
Homo sapiens | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | - |
Saccharomyces cerevisiae | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | - |
Candida albicans | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Homo sapiens | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Saccharomyces cerevisiae | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Candida albicans | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | Homo sapiens | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | Saccharomyces cerevisiae | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | Candida albicans | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | deficiency mutant fully swollen and some are lysed | Saccharomyces cerevisiae | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
N-acetyl-D-glucosamine 1-phosphate | R116A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 0.0177 | - |
N-acetyl-D-glucosamine 1-phosphate | G112A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 0.0848 | - |
N-acetyl-D-glucosamine 1-phosphate | K123A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 1.33 | - |
N-acetyl-D-glucosamine 1-phosphate | G111A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 2.27 | - |
N-acetyl-D-glucosamine 1-phosphate | L117A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 4.4 | - |
N-acetyl-D-glucosamine 1-phosphate | P122A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 5.31 | - |
N-acetyl-D-glucosamine 1-phosphate | G114A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 8.78 | - |
N-acetyl-D-glucosamine 1-phosphate | T115A mutant, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
| 15.4 | - |
N-acetyl-D-glucosamine 1-phosphate | wild type enzyme, 30°C, pH 8.3 | Saccharomyces cerevisiae | |
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