Cloned (Comment) | Organism |
---|---|
overexpression in host strain | Escherichia coli |
General Stability | Organism |
---|---|
completely insensitive to millimolar concentrations of thiol reagents | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP-N-acetyl-D-glucosamine | slight product inhibition in reverse reaction | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
N-acetyl-D-glucosamine 1-phosphate | pH 8.2, 37°C | Escherichia coli | |
0.1 | - |
UTP | pH 8.2, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for maximum activity | Escherichia coli | |
Mg2+ | optimum activity at 3 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
SDS-PAGE, corresponds very well with molecular weight expected from DNA sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-N-acetyl-D-glucosamine | Escherichia coli | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | Escherichia coli | involved in synthesis of peptidoglycan and lipopolysaccharide | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | Escherichia coli JM83 | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.1 | - |
purified enzyme, pH 8.2, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Escherichia coli | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | involved in synthesis of peptidoglycan and lipopolysaccharide | Escherichia coli | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli JM83 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Escherichia coli JM83 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
More | acetyltransferase 5 times higher than uridyltransferase activity | Escherichia coli |
More | bifunctional enzyme with activity of: glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Escherichia coli |