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Literature summary for 2.7.7.23 extracted from

  • Wang-Gillam, A.; Pastuszak, I.; Stewart, M.; Drake, R.R.; Elbein, A.D.
    Identification and modification of the uridine-binding site of the UDP-GalNAc (GlcNAc) pyrophosphorylase (2000), J. Biol. Chem., 275, 1433-1438.
    View publication on PubMed
Search for more literature for 2.7.7.23

Cloned(Commentary)

Cloned (Comment) Organism
AGX1 expressed in Escherichia coli as GST fusion protein Homo sapiens

Protein Variants

Protein Variants Comment Organism
G111A very low activity Homo sapiens
G222A traces of activity in forward and reverse reaction with N-acetyl-D-glucosamine 1-phosphate and N-acetyl-D-galactosamine 1-phosphate Homo sapiens
G224A low activity in forward and reverse reaction with N-acetyl-D-glucosamine 1-phosphate and N-acetyl-D-galactosamine 1-phosphate Homo sapiens
P220A only slight changes in activity with N-acetyl-D-glucosamine 1-phosphate and N-acetyl-D-galactosamine 1-phosphate Homo sapiens
R115A slight changes in Km Homo sapiens
Y227A only slight changes in activity with N-acetyl-D-glucosamine 1-phosphate and N-acetyl-D-galactosamine 1-phosphate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 UDP-N-acetyl-D-glucosamine AGX1, pH 8.5, 37°C Homo sapiens
0.8
-
 UDP-N-acetyl-D-glucosamine P220A, Y227A, R115A mutants, pH 8.5, 37°C Homo sapiens
2.2
-
 UDP-N-acetyl-D-glucosamine G224A mutants, pH 8.5, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + UDP-N-acetyl-D-glucosamine Homo sapiens amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine Homo sapiens involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GST fusion protein from E. coli Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + UDP-N-acetyl-D-glucosamine
-
 Homo sapiens UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Homo sapiens UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin Homo sapiens UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Synonyms

Synonyms Comment Organism
AGX1
-
 Homo sapiens