Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.23 extracted from

  • Mok, M.T.; Edwards, M.R.
    Kinetic and physical characterization of the inducible UDP-N-acetylglucosamine pyrophosphorylase from Giardia intestinalis (2005), J. Biol. Chem., 280, 39363-39372.
    View publication on PubMed
Search for more literature for 2.7.7.23

Cloned(Commentary)

Cloned (Comment) Organism
native and mutant enzymes expressed as His-tag fusion protein in Escherichia coli M15 Giardia intestinalis

Protein Variants

Protein Variants Comment Organism
G108A dramatically reduced activity, significant increase in melting temperature Giardia intestinalis
G210A dramatically reduced activity, significant decrease in melting temperature Giardia intestinalis

General Stability

General Stability Organism
substrate binding increases enzyme stability against thermal inactivation Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 N-acetyl-alpha-D-glucosamine 1-phosphate purified recombinant native enzyme, pH 7.5, 37°C Giardia intestinalis
0.038
-
 UTP Mg-UTP, purified recombinant native enzyme, pH 7.5, 37°C Giardia intestinalis

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ in complex with UTP Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 gel filtration Giardia intestinalis
49624
-
 1 * 50000, SDS-PAGE, 1 * 49624, calculated from the deduced amino acid sequence, native mass by gel filtration Giardia intestinalis
50000
-
 1 * 50000, SDS-PAGE, 1 * 49624, calculated from the deduced amino acid sequence, native mass by gel filtration Giardia intestinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Giardia intestinalis involved in the synthesis of UDP-N-acetyl-galactoseamine, which is important for cyst wall synthesis diphosphate + UDP-N-acetyl-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Giardia intestinalis Q8MUP8 Portland 1 strain, ATCC30888
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins using His-tag Giardia intestinalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
72
-
 purified recombinant native enzyme, pH 7.5, 37°C Giardia intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Giardia intestinalis diphosphate + UDP-N-acetyl-D-glucosamine
-
 ?
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate involved in the synthesis of UDP-N-acetyl-galactoseamine, which is important for cyst wall synthesis Giardia intestinalis diphosphate + UDP-N-acetyl-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE, 1 * 49624, calculated from the deduced amino acid sequence, native mass by gel filtration Giardia intestinalis