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Literature summary for 2.7.7.23 extracted from
- Purification and biochemical characterisation of GlmU from Yersinia pestis (2015), Arch. Microbiol., 197, 371-378.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene glmU, DNA and amino acid sequence determination, analysis, and comparison, functional complementation of the Escherichia coli glmU thermosensitive mutant strain UGS83, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Yersinia pseudotuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C307S | site-directed mutagenesis | Yersinia pseudotuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.061 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | pH 8.2, 37°C, recombinant His6-tagged enzyme | Yersinia pseudotuberculosis |  |
| 0.095 | - |
UTP | pH 8.2, 37°C, recombinant His6-tagged enzyme | Yersinia pseudotuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Yersinia pseudotuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52965 | - |
MALDI-TOF MS, SDS-PAGE | Yersinia pseudotuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | Yersinia pseudotuberculosis | - |
diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
| UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | Yersinia pseudotuberculosis YPIII | - |
diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yersinia pseudotuberculosis | - |
- |
- |
| Yersinia pseudotuberculosis YPIII | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Yersinia pseudotuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Yersinia pseudotuberculosis | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
| UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Yersinia pseudotuberculosis YPIII | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52965, recombinant His6-tagged enzyme, SDS-PAGE and mass spectrometry | Yersinia pseudotuberculosis |
| More | C324 does not form a disulphide bond with any other cysteine. This is consistent with the replacement of C307 by a serine in Y. pestis and the great distance between the three other cysteines (C296, C301 and C385) and C324 | Yersinia pseudotuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlcNAc-1-P uridyltransferase | - |
Yersinia pseudotuberculosis |
| GlmU | - |
Yersinia pseudotuberculosis |
| UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase | - |
Yersinia pseudotuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Yersinia pseudotuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
UTP | pH 8.2, 37°C, recombinant His6-tagged enzyme | Yersinia pseudotuberculosis | |
| 0.95 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | pH 8.2, 37°C, recombinant His6-tagged enzyme | Yersinia pseudotuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
assay at | Yersinia pseudotuberculosis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the GlmU proteins encoded by Yersinia pestis and Yersinia pseudotuberculosis are identical in amino acid sequence | Yersinia pseudotuberculosis |
| physiological function | the bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase enzyme GlmU is an essential gene in Yersinia | Yersinia pseudotuberculosis |
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